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Calcium in PDB 1os8: Recombinant Streptomyces Griseus Trypsin

Enzymatic activity of Recombinant Streptomyces Griseus Trypsin

All present enzymatic activity of Recombinant Streptomyces Griseus Trypsin:
3.4.21.4;

Protein crystallography data

The structure of Recombinant Streptomyces Griseus Trypsin, PDB code: 1os8 was solved by M.J.Page, S.L.Wong, J.Hewitt, N.C.Strynadka, R.T.Macgillivray, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 13.92 / 1.55
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 50.041, 69.824, 119.654, 90.00, 90.00, 90.00
R / Rfree (%) 19.6 / 22.2

Calcium Binding Sites:

The binding sites of Calcium atom in the Recombinant Streptomyces Griseus Trypsin (pdb code 1os8). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Recombinant Streptomyces Griseus Trypsin, PDB code: 1os8:

Calcium binding site 1 out of 1 in 1os8

Go back to Calcium Binding Sites List in 1os8
Calcium binding site 1 out of 1 in the Recombinant Streptomyces Griseus Trypsin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Recombinant Streptomyces Griseus Trypsin within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca249

b:15.0
occ:0.53
O A:GLU180 2.7 10.7 1.0
OE2 A:GLU230 2.9 14.9 1.0
O A:HOH459 3.0 18.7 1.0
O A:HOH380 3.0 20.0 1.0
O A:HOH458 3.4 15.4 1.0
CD A:GLU230 3.7 10.9 1.0
C A:GLU180 3.8 9.8 1.0
N A:ASN178 4.0 12.3 1.0
OE1 A:GLU230 4.1 16.6 1.0
CA A:ASN178 4.1 10.8 1.0
CA A:ILE181 4.3 10.1 1.0
O A:HOH258 4.4 9.9 1.0
N A:ILE181 4.4 9.6 1.0
N A:GLU180 4.5 8.6 1.0
OD2 A:ASP165 4.6 13.3 1.0
C A:ASN178 4.6 12.2 1.0
CB A:ILE181 4.7 14.0 1.0
CG A:GLU230 4.8 9.8 1.0
CA A:GLU180 4.8 9.0 1.0
N A:GLU179 4.8 9.6 1.0
CB A:ASP165 4.9 10.4 1.0
CB A:GLU230 5.0 7.5 1.0
O A:HOH396 5.0 23.6 1.0

Reference:

M.J.Page, S.L.Wong, J.Hewitt, N.C.Strynadka, R.T.Macgillivray. Engineering the Primary Substrate Specificity of Streptomyces Griseus Trypsin. Biochemistry V. 42 9060 2003.
ISSN: ISSN 0006-2960
PubMed: 12885239
DOI: 10.1021/BI0344230
Page generated: Sat Dec 12 03:12:55 2020

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