Calcium in PDB 1oss: T190P Streptomyces Griseus Trypsin in Complex with Benzamidine

All present enzymatic activity of T190P Streptomyces Griseus Trypsin in Complex with Benzamidine:
3.4.21.4;

The structure of T190P Streptomyces Griseus Trypsin in Complex with Benzamidine, PDB code: 1oss was solved by M.J.Page, S.L.Wong, J.Hewitt, N.C.Strynadka, R.T.Macgillivray, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.86 / 1.93
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	50.079, 69.601, 119.829, 90.00, 90.00, 90.00
R / Rfree (%)	16.7 / 21.2

The binding sites of Calcium atom in the T190P Streptomyces Griseus Trypsin in Complex with Benzamidine (pdb code 1oss). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the T190P Streptomyces Griseus Trypsin in Complex with Benzamidine, PDB code: 1oss:

Calcium binding site 1 out of 1 in the T190P Streptomyces Griseus Trypsin in Complex with Benzamidine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of T190P Streptomyces Griseus Trypsin in Complex with Benzamidine within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca249 b:17.5 occ:0.43 OD1 A:ASP165 2.6 17.4 0.5 O A:GLU180 2.8 10.9 1.0 O A:HOH294 2.9 41.4 1.0 OD2 A:ASP165 3.0 17.3 0.5 OE2 A:GLU230 3.0 16.9 1.0 CG A:ASP165 3.2 14.6 0.5 C A:GLU180 3.8 9.3 1.0 CD A:GLU230 3.9 12.0 1.0 N A:ASN178 4.0 16.3 1.0 CA A:ASN178 4.3 16.0 1.0 OE1 A:GLU230 4.3 13.9 1.0 O A:HOH257 4.3 6.0 1.0 CA A:ILE181 4.4 9.9 1.0 OD2 A:ASP165 4.5 8.9 0.5 N A:ILE181 4.5 8.4 1.0 CB A:ASP165 4.6 11.7 0.5 N A:GLU180 4.7 8.3 1.0 CB A:ASP165 4.7 8.2 0.5 CB A:ILE181 4.7 11.1 1.0 C A:ASN178 4.7 13.6 1.0 CA A:GLU180 4.9 8.9 1.0 N A:GLU179 4.9 11.5 1.0 CG A:GLU230 5.0 9.7 1.0

M.J.Page, S.L.Wong, J.Hewitt, N.C.Strynadka, R.T.Macgillivray. Engineering the Primary Substrate Specificity of Streptomyces Griseus Trypsin. Biochemistry V. 42 9060 2003.
ISSN: ISSN 0006-2960
PubMed: 12885239
DOI: 10.1021/BI0344230