Calcium in PDB 1poo: Thermostable Phytase From Bacillus Sp

All present enzymatic activity of Thermostable Phytase From Bacillus Sp:
3.1.3.8;

The structure of Thermostable Phytase From Bacillus Sp, PDB code: 1poo was solved by B.H.Oh, N.C.Ha, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 2.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.100, 64.800, 105.600, 90.00, 90.00, 90.00
R / Rfree (%)	21.4 / 29.1

The binding sites of Calcium atom in the Thermostable Phytase From Bacillus Sp (pdb code 1poo). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Thermostable Phytase From Bacillus Sp, PDB code: 1poo:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Thermostable Phytase From Bacillus Sp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Thermostable Phytase From Bacillus Sp within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca801 b:24.7 occ:1.00 OD1 A:ASP308 2.4 18.5 1.0 OD1 A:ASP341 2.5 31.8 1.0 OE1 A:GLU43 2.6 22.7 1.0 OD1 A:ASN339 2.7 21.4 1.0 OE2 A:GLU43 2.7 24.8 1.0 O A:ILE340 2.8 34.6 1.0 CD A:GLU43 3.0 18.2 1.0 O A:HOH529 3.1 23.7 1.0 CG A:ASP308 3.4 20.2 1.0 C A:ILE340 3.5 29.4 1.0 CG A:ASP341 3.7 33.0 1.0 CG A:ASN339 3.8 24.1 1.0 NZ A:LYS351 4.0 13.2 1.0 CA A:ASP341 4.1 36.4 1.0 N A:ASP341 4.1 35.9 1.0 OD2 A:ASP308 4.1 21.6 1.0 N A:ILE340 4.1 26.6 1.0 CB A:ASP308 4.3 23.1 1.0 ND2 A:ASN339 4.3 25.3 1.0 N A:ASP308 4.3 21.9 1.0 CA A:ILE340 4.5 26.6 1.0 CB A:ASP341 4.5 37.2 1.0 CG A:GLU43 4.5 19.4 1.0 OD2 A:ASP341 4.6 34.7 1.0 CE A:LYS351 4.6 11.0 1.0 OD1 A:ASN349 4.7 21.7 1.0 C A:ASN339 4.9 22.5 1.0 CA A:ASP308 4.9 20.9 1.0 CB A:ASN339 5.0 21.8 1.0

Calcium binding site 2 out of 2 in the Thermostable Phytase From Bacillus Sp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Thermostable Phytase From Bacillus Sp within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca802 b:31.5 occ:1.00 OD2 A:ASP56 2.5 20.1 1.0 O A:HOH533 2.6 65.5 1.0 O A:VAL101 2.6 23.4 1.0 O A:HOH515 2.7 17.4 1.0 O A:HOH526 2.7 20.3 1.0 O A:PRO57 2.8 16.7 1.0 OD1 A:ASP56 3.1 21.0 1.0 CG A:ASP56 3.1 17.0 1.0 C A:VAL101 3.8 14.3 1.0 C A:PRO57 3.9 10.8 1.0 O A:HOH534 4.4 13.7 1.0 CA A:ASP102 4.4 9.3 1.0 N A:ASP102 4.5 12.8 1.0 O A:HOH504 4.6 27.3 1.0 CB A:ASP56 4.6 13.9 1.0 CB A:ALA58 4.6 18.1 1.0 N A:VAL101 4.6 14.2 1.0 O A:ILE316 4.6 17.8 1.0 CA A:ALA58 4.6 12.3 1.0 N A:PRO57 4.6 12.3 1.0 CB A:ASP102 4.7 8.9 1.0 CG A:ASP317 4.7 9.5 1.0 N A:ALA58 4.7 11.9 1.0 OD2 A:ASP317 4.7 18.6 1.0 CA A:VAL101 4.8 9.0 1.0 CG1 A:VAL101 4.9 11.3 1.0 CG A:ASP102 4.9 8.1 1.0 CB A:ASP317 4.9 11.2 1.0 OD1 A:ASP102 4.9 20.1 1.0 CA A:PRO57 4.9 11.1 1.0 CD A:PRO57 5.0 13.9 1.0 OD1 A:ASP317 5.0 12.6 1.0

N.C.Ha, B.C.Oh, S.Shin, H.J.Kim, T.K.Oh, Y.O.Kim, K.Y.Choi, B.H.Oh. Crystal Structures of A Novel, Thermostable Phytase in Partially and Fully Calcium-Loaded States. Nat.Struct.Biol. V. 7 147 2000.
ISSN: ISSN 1072-8368
PubMed: 10655618
DOI: 10.1038/72421