Atomistry » Calcium » PDB 1px7-1qcp » 1pzy
Atomistry »
  Calcium »
    PDB 1px7-1qcp »
      1pzy »

Calcium in PDB 1pzy: W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese

Enzymatic activity of W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese

All present enzymatic activity of W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese:
2.4.1.22; 2.4.1.38; 2.4.1.90;

Protein crystallography data

The structure of W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese, PDB code: 1pzy was solved by V.Ramasamy, B.Ramakrishnan, E.Boeggeman, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.95 / 2.30
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 55.292, 98.747, 102.788, 90.00, 103.92, 90.00
R / Rfree (%) 20.7 / 25.3

Other elements in 1pzy:

The structure of W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese also contains other interesting chemical elements:

Manganese (Mn) 2 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese (pdb code 1pzy). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese, PDB code: 1pzy:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 1pzy

Go back to Calcium Binding Sites List in 1pzy
Calcium binding site 1 out of 2 in the W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca403

b:36.8
occ:1.00
 O A:LYS79 2.2 39.1 1.0
O A:HOH590 2.3 38.3 1.0
O A:GLU84 2.3 40.8 1.0
OD1 A:ASP87 2.4 36.1 1.0
OD2 A:ASP88 2.5 28.9 1.0
OD1 A:ASP82 2.5 39.5 1.0
O A:HOH473 2.6 37.2 1.0
CG A:ASP82 3.2 40.9 1.0
CG A:ASP87 3.3 37.6 1.0
OD2 A:ASP82 3.3 41.7 1.0
C A:GLU84 3.4 42.4 1.0
C A:LYS79 3.4 37.7 1.0
OD2 A:ASP87 3.5 36.8 1.0
CG A:ASP88 3.5 31.1 1.0
OD1 A:ASP88 3.9 32.0 1.0
CA A:LEU80 4.1 35.5 1.0
CB A:GLU84 4.1 47.1 1.0
CA A:GLU84 4.2 44.9 1.0
N A:GLU84 4.2 44.7 1.0
N A:LEU80 4.2 36.7 1.0
N A:LEU85 4.4 40.6 1.0
CA A:LEU85 4.5 38.3 1.0
N A:ASP88 4.5 30.2 1.0
CA A:LYS79 4.5 37.2 1.0
N A:ASP82 4.6 38.8 1.0
C A:LEU80 4.6 34.4 1.0
CB A:ASP82 4.6 39.9 1.0
CB A:ASP87 4.7 35.7 1.0
C A:ASP82 4.8 41.4 1.0
O A:ASP82 4.8 42.2 1.0
CB A:LYS79 4.8 41.2 1.0
CB A:ASP88 4.8 31.0 1.0
N A:ASP87 4.8 37.9 1.0
N A:LEU81 4.9 34.4 1.0
CA A:ASP82 4.9 40.5 1.0
CG A:GLU84 5.0 53.4 1.0
C A:LEU85 5.0 38.2 1.0

Calcium binding site 2 out of 2 in 1pzy

Go back to Calcium Binding Sites List in 1pzy
Calcium binding site 2 out of 2 in the W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca404

b:49.2
occ:1.00
 O C:GLU84 2.2 43.3 1.0
O C:LYS79 2.3 53.5 1.0
OD1 C:ASP87 2.5 43.9 1.0
OD1 C:ASP82 2.6 51.3 1.0
OD2 C:ASP88 2.6 46.3 1.0
O C:HOH582 2.6 32.8 1.0
O C:HOH581 2.7 35.0 1.0
CG C:ASP82 3.3 51.8 1.0
CG C:ASP87 3.3 44.1 1.0
C C:GLU84 3.3 45.8 1.0
OD2 C:ASP87 3.4 42.9 1.0
OD2 C:ASP82 3.4 51.1 1.0
C C:LYS79 3.5 54.7 1.0
CG C:ASP88 3.7 43.3 1.0
OD1 C:ASP88 4.0 41.3 1.0
CA C:LEU80 4.1 52.0 1.0
CA C:GLU84 4.1 46.5 1.0
N C:GLU84 4.2 46.6 1.0
CB C:GLU84 4.2 46.7 1.0
N C:LEU80 4.3 53.5 1.0
N C:LEU85 4.3 46.7 1.0
CA C:LEU85 4.4 48.1 1.0
N C:ASP82 4.5 49.1 1.0
CA C:LYS79 4.6 54.4 1.0
C C:LEU80 4.6 51.3 1.0
CB C:ASP82 4.6 50.6 1.0
O C:ASP82 4.7 51.3 1.0
N C:ASP88 4.7 44.9 1.0
N C:LEU81 4.7 49.2 1.0
CB C:ASP87 4.7 44.6 1.0
C C:ASP82 4.8 50.1 1.0
CB C:LYS79 4.8 56.6 1.0
N C:ASP87 4.9 47.0 1.0
CA C:ASP82 4.9 50.0 1.0
CB C:ASP88 4.9 43.5 1.0
CD2 C:LEU80 5.0 46.5 1.0
C C:LEU85 5.0 48.4 1.0

Reference:

V.Ramasamy, B.Ramakrishnan, E.Boeggeman, P.K.Qasba. The Role of Tryptophan 314 in the Conformational Changes of Beta 1,4-Galactosyltransferase-I J.Mol.Biol. V. 331 1065 2003.
ISSN: ISSN 0022-2836
PubMed: 12927542
DOI: 10.1016/S0022-2836(03)00790-3
Page generated: Thu Jul 11 13:57:51 2024

Last articles

Zn in 9J0N
Zn in 9J0O
Zn in 9J0P
Zn in 9FJX
Zn in 9EKB
Zn in 9C0F
Zn in 9CAH
Zn in 9CH0
Zn in 9CH3
Zn in 9CH1
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy