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Calcium in PDB 1pzy: W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese

Enzymatic activity of W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese

All present enzymatic activity of W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese:
2.4.1.22; 2.4.1.38; 2.4.1.90;

Protein crystallography data

The structure of W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese, PDB code: 1pzy was solved by V.Ramasamy, B.Ramakrishnan, E.Boeggeman, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.95 / 2.30
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 55.292, 98.747, 102.788, 90.00, 103.92, 90.00
R / Rfree (%) 20.7 / 25.3

Other elements in 1pzy:

The structure of W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese also contains other interesting chemical elements:

Manganese (Mn) 2 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese (pdb code 1pzy). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese, PDB code: 1pzy:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 1pzy

Go back to Calcium Binding Sites List in 1pzy
Calcium binding site 1 out of 2 in the W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca403

b:36.8
occ:1.00
 O A:LYS79 2.2 39.1 1.0
O A:HOH590 2.3 38.3 1.0
O A:GLU84 2.3 40.8 1.0
OD1 A:ASP87 2.4 36.1 1.0
OD2 A:ASP88 2.5 28.9 1.0
OD1 A:ASP82 2.5 39.5 1.0
O A:HOH473 2.6 37.2 1.0
CG A:ASP82 3.2 40.9 1.0
CG A:ASP87 3.3 37.6 1.0
OD2 A:ASP82 3.3 41.7 1.0
C A:GLU84 3.4 42.4 1.0
C A:LYS79 3.4 37.7 1.0
OD2 A:ASP87 3.5 36.8 1.0
CG A:ASP88 3.5 31.1 1.0
OD1 A:ASP88 3.9 32.0 1.0
CA A:LEU80 4.1 35.5 1.0
CB A:GLU84 4.1 47.1 1.0
CA A:GLU84 4.2 44.9 1.0
N A:GLU84 4.2 44.7 1.0
N A:LEU80 4.2 36.7 1.0
N A:LEU85 4.4 40.6 1.0
CA A:LEU85 4.5 38.3 1.0
N A:ASP88 4.5 30.2 1.0
CA A:LYS79 4.5 37.2 1.0
N A:ASP82 4.6 38.8 1.0
C A:LEU80 4.6 34.4 1.0
CB A:ASP82 4.6 39.9 1.0
CB A:ASP87 4.7 35.7 1.0
C A:ASP82 4.8 41.4 1.0
O A:ASP82 4.8 42.2 1.0
CB A:LYS79 4.8 41.2 1.0
CB A:ASP88 4.8 31.0 1.0
N A:ASP87 4.8 37.9 1.0
N A:LEU81 4.9 34.4 1.0
CA A:ASP82 4.9 40.5 1.0
CG A:GLU84 5.0 53.4 1.0
C A:LEU85 5.0 38.2 1.0

Calcium binding site 2 out of 2 in 1pzy

Go back to Calcium Binding Sites List in 1pzy
Calcium binding site 2 out of 2 in the W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of W314A-BETA1,4-Galactosyltransferase-I Complexed with Alpha-Lactalbumin in the Presence of N-Acetylglucosamine, Udp and Manganese within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca404

b:49.2
occ:1.00
 O C:GLU84 2.2 43.3 1.0
O C:LYS79 2.3 53.5 1.0
OD1 C:ASP87 2.5 43.9 1.0
OD1 C:ASP82 2.6 51.3 1.0
OD2 C:ASP88 2.6 46.3 1.0
O C:HOH582 2.6 32.8 1.0
O C:HOH581 2.7 35.0 1.0
CG C:ASP82 3.3 51.8 1.0
CG C:ASP87 3.3 44.1 1.0
C C:GLU84 3.3 45.8 1.0
OD2 C:ASP87 3.4 42.9 1.0
OD2 C:ASP82 3.4 51.1 1.0
C C:LYS79 3.5 54.7 1.0
CG C:ASP88 3.7 43.3 1.0
OD1 C:ASP88 4.0 41.3 1.0
CA C:LEU80 4.1 52.0 1.0
CA C:GLU84 4.1 46.5 1.0
N C:GLU84 4.2 46.6 1.0
CB C:GLU84 4.2 46.7 1.0
N C:LEU80 4.3 53.5 1.0
N C:LEU85 4.3 46.7 1.0
CA C:LEU85 4.4 48.1 1.0
N C:ASP82 4.5 49.1 1.0
CA C:LYS79 4.6 54.4 1.0
C C:LEU80 4.6 51.3 1.0
CB C:ASP82 4.6 50.6 1.0
O C:ASP82 4.7 51.3 1.0
N C:ASP88 4.7 44.9 1.0
N C:LEU81 4.7 49.2 1.0
CB C:ASP87 4.7 44.6 1.0
C C:ASP82 4.8 50.1 1.0
CB C:LYS79 4.8 56.6 1.0
N C:ASP87 4.9 47.0 1.0
CA C:ASP82 4.9 50.0 1.0
CB C:ASP88 4.9 43.5 1.0
CD2 C:LEU80 5.0 46.5 1.0
C C:LEU85 5.0 48.4 1.0

Reference:

V.Ramasamy, B.Ramakrishnan, E.Boeggeman, P.K.Qasba. The Role of Tryptophan 314 in the Conformational Changes of Beta 1,4-Galactosyltransferase-I J.Mol.Biol. V. 331 1065 2003.
ISSN: ISSN 0022-2836
PubMed: 12927542
DOI: 10.1016/S0022-2836(03)00790-3
Page generated: Thu Jul 11 13:57:51 2024

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