Calcium in PDB 1rgi: Crystal Structure of Gelsolin Domains G1-G3 Bound to Actin

The structure of Crystal Structure of Gelsolin Domains G1-G3 Bound to Actin, PDB code: 1rgi was solved by L.D.Burtnick, D.Urosev, E.Irobi, K.Narayan, R.C.Robinson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.96 / 3.00
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	145.245, 145.245, 129.948, 90.00, 90.00, 120.00
R / Rfree (%)	22.4 / 25.8

The binding sites of Calcium atom in the Crystal Structure of Gelsolin Domains G1-G3 Bound to Actin (pdb code 1rgi). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Crystal Structure of Gelsolin Domains G1-G3 Bound to Actin, PDB code: 1rgi:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Crystal Structure of Gelsolin Domains G1-G3 Bound to Actin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Gelsolin Domains G1-G3 Bound to Actin within 5.0Å range: probe atom residue distance (Å) B Occ G:Ca403 b:25.9 occ:1.00 O G:GLY65 2.2 23.6 1.0 O G:VAL145 2.3 27.6 1.0 OE1 G:GLU97 2.4 29.7 1.0 OD2 G:ASP66 2.4 24.2 1.0 OE2 G:GLU97 2.9 32.2 1.0 CD G:GLU97 3.0 29.3 1.0 C G:GLY65 3.3 23.5 1.0 C G:VAL145 3.4 27.3 1.0 CG G:ASP66 3.6 25.0 1.0 N G:VAL145 3.9 26.5 1.0 C G:GLY144 4.1 25.6 1.0 CA G:GLY65 4.2 22.9 1.0 N G:ASP66 4.2 23.6 1.0 CA G:ASP66 4.3 23.5 1.0 N G:ALA146 4.3 27.8 1.0 CA G:GLY144 4.3 24.9 1.0 CA G:VAL145 4.3 27.2 1.0 CA G:ALA146 4.4 28.1 1.0 CG G:GLU97 4.4 26.8 1.0 OD1 G:ASP66 4.4 23.6 1.0 C G:ALA146 4.5 28.5 1.0 N G:SER147 4.5 28.7 1.0 CB G:ASP66 4.6 23.9 1.0 O G:GLY144 4.6 25.9 1.0 CG1 G:VAL145 4.7 28.0 1.0 N G:SER94 4.8 28.7 1.0 OG G:SER94 4.9 27.5 1.0 CA G:CYS93 4.9 30.1 1.0 O G:ALA146 5.0 29.1 1.0

Calcium binding site 2 out of 4 in the Crystal Structure of Gelsolin Domains G1-G3 Bound to Actin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Gelsolin Domains G1-G3 Bound to Actin within 5.0Å range: probe atom residue distance (Å) B Occ G:Ca404 b:36.3 occ:1.00 OE1 A:GLU167 2.3 31.3 1.0 O G:GLY114 2.4 32.8 1.0 OD2 G:ASP109 2.6 33.0 1.0 O A:HOH402 2.6 27.3 1.0 O G:ALA116 3.0 29.9 1.0 C G:GLY114 3.3 33.0 1.0 CG G:ASP109 3.4 32.0 1.0 OD1 G:ASP109 3.4 33.6 1.0 CD A:GLU167 3.5 30.0 1.0 CA G:GLY114 3.8 33.2 1.0 C G:ALA116 4.0 29.7 1.0 OE2 A:GLU167 4.1 31.6 1.0 N G:ALA116 4.2 31.0 1.0 N G:ARG115 4.5 32.9 1.0 CG A:GLU167 4.6 28.3 1.0 CB A:GLU167 4.6 27.4 1.0 CA G:ALA116 4.7 30.3 1.0 NE2 G:GLN118 4.8 21.3 1.0 CB G:ASP109 4.8 30.8 1.0 C G:ARG115 5.0 32.2 1.0 CA G:ARG115 5.0 33.1 1.0 N G:VAL117 5.0 28.8 1.0

Calcium binding site 3 out of 4 in the Crystal Structure of Gelsolin Domains G1-G3 Bound to Actin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Gelsolin Domains G1-G3 Bound to Actin within 5.0Å range: probe atom residue distance (Å) B Occ G:Ca406 b:47.9 occ:1.00 O G:GLU302 2.6 31.9 1.0 OE2 G:GLU327 2.7 33.0 1.0 OE1 G:GLU327 2.8 31.7 1.0 OD1 G:ASP303 3.0 31.1 1.0 CD G:GLU327 3.1 31.4 1.0 C G:GLU302 3.7 31.7 1.0 OD1 G:ASN278 3.9 22.3 1.0 ND2 G:ASN324 4.1 29.7 1.0 CG G:ASP303 4.2 32.4 1.0 OG G:SER277 4.2 27.3 1.0 CB G:GLU302 4.3 32.8 1.0 CA G:GLU302 4.4 32.4 1.0 CA G:GLY279 4.5 23.5 1.0 CG G:GLU327 4.5 30.8 1.0 N G:ASP303 4.6 31.1 1.0 CA G:ASP303 4.6 31.1 1.0 N G:GLY279 4.6 23.6 1.0 CB G:ASP303 4.9 31.2 1.0 CA G:ALA323 5.0 27.3 1.0 N G:ASN324 5.0 27.6 1.0 N G:ASN278 5.0 24.3 1.0

Calcium binding site 4 out of 4 in the Crystal Structure of Gelsolin Domains G1-G3 Bound to Actin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of Gelsolin Domains G1-G3 Bound to Actin within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:31.1 occ:1.00 O3G A:ATP380 2.5 26.8 1.0 O2B A:ATP380 2.5 21.9 1.0 OE1 A:GLN137 3.7 27.9 1.0 PG A:ATP380 3.8 26.2 1.0 PB A:ATP380 4.0 19.9 1.0 O1G A:ATP380 4.0 23.9 1.0 OD2 A:ASP154 4.3 40.3 1.0 OD1 A:ASP154 4.3 40.2 1.0 O3B A:ATP380 4.4 23.2 1.0 CD A:GLN137 4.4 28.0 1.0 O1A A:ATP380 4.4 22.0 1.0 CG A:ASP154 4.6 37.4 1.0 OD2 A:ASP11 4.6 38.0 1.0 CA A:GLY13 4.6 28.2 1.0 O3A A:ATP380 4.7 21.8 1.0 O1B A:ATP380 4.9 17.4 1.0 O2G A:ATP380 4.9 22.4 1.0 NE2 A:GLN137 4.9 26.5 1.0 OD1 A:ASP11 5.0 36.1 1.0

L.D.Burtnick, D.Urosev, E.Irobi, K.Narayan, R.C.Robinson. Structure of the N-Terminal Half of Gelsolin Bound to Actin: Roles in Severing, Apoptosis and Faf Embo J. V. 23 2713 2004.
ISSN: ISSN 0261-4189
PubMed: 15215896
DOI: 10.1038/SJ.EMBOJ.7600280