Calcium in PDB 1rgk: Rnase T1 Mutant GLU46GLN Binds the Inhibitors 2'Gmp and 2'Amp at the 3' Subsite

All present enzymatic activity of Rnase T1 Mutant GLU46GLN Binds the Inhibitors 2'Gmp and 2'Amp at the 3' Subsite:
3.1.27.3;

The structure of Rnase T1 Mutant GLU46GLN Binds the Inhibitors 2'Gmp and 2'Amp at the 3' Subsite, PDB code: 1rgk was solved by J.Granzin, R.Puras-Lutzke, O.Landt, H.-P.Grunert, U.Heinemann, W.Saenger, U.Hahn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	N/A / 1.87
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	49.100, 46.690, 41.110, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Calcium atom in the Rnase T1 Mutant GLU46GLN Binds the Inhibitors 2'Gmp and 2'Amp at the 3' Subsite (pdb code 1rgk). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Rnase T1 Mutant GLU46GLN Binds the Inhibitors 2'Gmp and 2'Amp at the 3' Subsite, PDB code: 1rgk:

Calcium binding site 1 out of 1 in the Rnase T1 Mutant GLU46GLN Binds the Inhibitors 2'Gmp and 2'Amp at the 3' Subsite


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Rnase T1 Mutant GLU46GLN Binds the Inhibitors 2'Gmp and 2'Amp at the 3' Subsite within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca106 b:16.7 occ:1.00 O A:HOH145 2.4 24.5 1.0 O A:HOH115 2.4 18.3 1.0 O A:HOH110 2.4 14.2 1.0 OD2 A:ASP15 2.5 12.0 1.0 O A:HOH116 2.5 17.4 1.0 O A:HOH114 2.5 13.4 1.0 O A:HOH191 2.6 46.0 1.0 OD1 A:ASP15 2.6 14.1 1.0 CG A:ASP15 2.9 12.2 1.0 CB A:ASP15 4.3 11.6 1.0 O A:HOH137 4.4 23.5 1.0 O A:HOH184 4.5 49.0 1.0 OG A:SER12 4.6 13.8 1.0 O A:CYS10 4.7 15.6 1.0 O A:HOH118 4.7 15.4 1.0 CA A:GLY65 4.8 16.6 1.0 O A:HOH139 4.9 21.8 1.0 N A:SER12 4.9 12.2 1.0

J.Granzin, R.Puras-Lutzke, O.Landt, H.P.Grunert, U.Heinemann, W.Saenger, U.Hahn. Rnase T1 Mutant GLU46GLN Binds the Inhibitors 2'Gmp and 2'Amp at the 3' Subsite. J.Mol.Biol. V. 225 533 1992.
ISSN: ISSN 0022-2836
PubMed: 1350642
DOI: 10.1016/0022-2836(92)90937-F