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Calcium in PDB 1rls: Crystal Structure of Rnase T1 Complexed with the Product Nucleotide 3'-Gmp. Structural Evidence For Direct Interaction of Histidine 40 and Glutamic Acid 58 with the 2'-Hydroxyl Group of Ribose

Enzymatic activity of Crystal Structure of Rnase T1 Complexed with the Product Nucleotide 3'-Gmp. Structural Evidence For Direct Interaction of Histidine 40 and Glutamic Acid 58 with the 2'-Hydroxyl Group of Ribose

All present enzymatic activity of Crystal Structure of Rnase T1 Complexed with the Product Nucleotide 3'-Gmp. Structural Evidence For Direct Interaction of Histidine 40 and Glutamic Acid 58 with the 2'-Hydroxyl Group of Ribose:
3.1.27.3;

Protein crystallography data

The structure of Crystal Structure of Rnase T1 Complexed with the Product Nucleotide 3'-Gmp. Structural Evidence For Direct Interaction of Histidine 40 and Glutamic Acid 58 with the 2'-Hydroxyl Group of Ribose, PDB code: 1rls was solved by K.Gohda, K.-I.Oka, K.-I.Tomita, T.Hakoshima, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.00 / 1.90
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 48.140, 51.060, 40.380, 90.00, 90.00, 90.00
R / Rfree (%) n/a / n/a

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of Rnase T1 Complexed with the Product Nucleotide 3'-Gmp. Structural Evidence For Direct Interaction of Histidine 40 and Glutamic Acid 58 with the 2'-Hydroxyl Group of Ribose (pdb code 1rls). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Rnase T1 Complexed with the Product Nucleotide 3'-Gmp. Structural Evidence For Direct Interaction of Histidine 40 and Glutamic Acid 58 with the 2'-Hydroxyl Group of Ribose, PDB code: 1rls:

Calcium binding site 1 out of 1 in 1rls

Go back to Calcium Binding Sites List in 1rls
Calcium binding site 1 out of 1 in the Crystal Structure of Rnase T1 Complexed with the Product Nucleotide 3'-Gmp. Structural Evidence For Direct Interaction of Histidine 40 and Glutamic Acid 58 with the 2'-Hydroxyl Group of Ribose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Rnase T1 Complexed with the Product Nucleotide 3'-Gmp. Structural Evidence For Direct Interaction of Histidine 40 and Glutamic Acid 58 with the 2'-Hydroxyl Group of Ribose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca106

b:23.2
occ:1.00
 O A:HOH239 2.3 33.5 1.0
OD2 A:ASP15 2.4 6.9 1.0
O A:HOH214 2.6 13.1 1.0
OD1 A:ASP15 2.6 16.2 1.0
O A:HOH216 2.7 23.6 1.0
CG A:ASP15 2.8 8.2 1.0
O A:HOH204 2.9 22.1 1.0
O A:HOH252 3.2 15.2 1.0
O A:HOH290 4.2 23.6 1.0
CB A:ASP15 4.3 5.8 1.0
O A:HOH241 4.4 34.2 1.0
O A:CYS10 4.7 8.0 1.0
OG A:SER12 4.7 12.4 1.0
O A:HOH249 4.9 10.8 1.0
N A:SER12 5.0 11.1 1.0

Reference:

K.Gohda, K.Oka, K.Tomita, T.Hakoshima. Crystal Structure of Rnase T1 Complexed with the Product Nucleotide 3'-Gmp. Structural Evidence For Direct Interaction of Histidine 40 and Glutamic Acid 58 with the 2'-Hydroxyl Group of the Ribose. J.Biol.Chem. V. 269 17531 1994.
ISSN: ISSN 0021-9258
PubMed: 7912696
Page generated: Sat Dec 12 03:17:53 2020

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