Calcium in PDB 1s02: Effects of Engineered Salt Bridges on the Stability of Subtilisin Bpn'

All present enzymatic activity of Effects of Engineered Salt Bridges on the Stability of Subtilisin Bpn':
3.4.21.14;

The structure of Effects of Engineered Salt Bridges on the Stability of Subtilisin Bpn', PDB code: 1s02 was solved by C.R.Erwin, B.L.Barnett, J.D.Oliver, J.F.Sullivan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	N/A / 1.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	39.300, 72.900, 75.400, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Calcium atom in the Effects of Engineered Salt Bridges on the Stability of Subtilisin Bpn' (pdb code 1s02). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Effects of Engineered Salt Bridges on the Stability of Subtilisin Bpn', PDB code: 1s02:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Effects of Engineered Salt Bridges on the Stability of Subtilisin Bpn'


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Effects of Engineered Salt Bridges on the Stability of Subtilisin Bpn' within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca276 b:3.8 occ:1.00 O A:ILE79 2.2 7.2 1.0 OE1 A:GLN2 2.2 8.7 1.0 O A:LEU75 2.3 8.0 1.0 O A:VAL81 2.3 7.5 1.0 OD1 A:ASP41 2.4 6.0 1.0 OD1 A:ASN77 2.4 7.5 1.0 OD2 A:ASP41 2.6 6.5 1.0 CG A:ASP41 2.9 6.2 1.0 CG A:ASN77 3.3 8.6 1.0 CD A:GLN2 3.4 9.4 1.0 C A:ILE79 3.4 8.7 1.0 C A:LEU75 3.5 8.0 1.0 C A:VAL81 3.5 7.1 1.0 N A:VAL81 3.8 7.6 1.0 ND2 A:ASN77 3.8 8.1 1.0 N A:ASN77 3.9 9.8 1.0 CG A:GLN2 3.9 9.5 1.0 C A:GLY80 4.1 7.6 1.0 CA A:VAL81 4.2 7.1 1.0 N A:GLY80 4.3 8.2 1.0 CB A:GLN2 4.3 10.4 1.0 CA A:LEU75 4.3 7.7 1.0 N A:ILE79 4.4 10.6 1.0 N A:LEU75 4.4 7.0 1.0 CB A:ASP41 4.4 6.5 1.0 CA A:GLY80 4.4 8.0 1.0 N A:ASN76 4.4 9.0 1.0 CA A:ILE79 4.4 9.2 1.0 CG1 A:ILE79 4.4 10.6 1.0 NE2 A:GLN2 4.5 9.3 1.0 N A:LEU82 4.6 6.6 1.0 CA A:ASN77 4.6 9.8 1.0 CB A:ASN77 4.6 9.1 1.0 CA A:ASN76 4.6 9.5 1.0 C A:ASN77 4.7 10.0 1.0 C A:ALA74 4.7 6.1 1.0 C A:ASN76 4.7 10.0 1.0 CB A:LEU75 4.7 7.7 1.0 O A:GLY80 4.8 7.6 1.0 O A:ASN77 4.8 10.4 1.0 CA A:LEU82 4.9 6.6 1.0 O A:ALA74 4.9 6.6 1.0

Calcium binding site 2 out of 2 in the Effects of Engineered Salt Bridges on the Stability of Subtilisin Bpn'


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Effects of Engineered Salt Bridges on the Stability of Subtilisin Bpn' within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca277 b:20.8 occ:0.60 O A:VAL174 2.5 8.2 1.0 O A:GLY169 2.9 12.1 1.0 O A:TYR171 2.9 6.7 1.0 OD2 A:ASP197 2.9 6.9 1.0 O A:HOH335 3.1 17.1 1.0 O A:GLU195 3.4 8.5 1.0 C A:VAL174 3.6 8.5 1.0 C A:TYR171 3.8 7.5 1.0 CG A:ASP197 3.9 7.2 1.0 CB A:ALA176 3.9 5.8 1.0 N A:ALA176 3.9 6.2 1.0 OD1 A:ASP197 4.1 8.1 1.0 C A:GLY169 4.1 11.5 1.0 NH2 A:ARG247 4.2 8.3 1.0 CA A:PRO172 4.3 7.3 1.0 O A:LYS170 4.4 10.0 1.0 O A:PRO172 4.4 7.6 1.0 N A:PRO172 4.4 7.2 1.0 C A:LYS170 4.4 10.3 1.0 CA A:VAL174 4.4 8.9 1.0 N A:VAL174 4.4 8.7 1.0 C A:PRO172 4.5 7.5 1.0 C A:GLU195 4.5 8.9 1.0 N A:ILE175 4.6 8.0 1.0 CA A:ALA176 4.6 5.9 1.0 CB A:GLU195 4.6 9.3 1.0 N A:TYR171 4.6 9.3 1.0 CB A:VAL174 4.6 8.9 1.0 CA A:ILE175 4.6 7.4 1.0 C A:ILE175 4.7 7.0 1.0 CA A:GLU195 4.8 8.9 1.0 CA A:LYS170 4.9 11.0 1.0 CA A:TYR171 4.9 8.2 1.0 N A:LYS170 4.9 11.2 1.0

C.R.Erwin, B.L.Barnett, J.D.Oliver, J.F.Sullivan. Effects of Engineered Salt Bridges on the Stability of Subtilisin Bpn'. Protein Eng. V. 4 87 1990.
ISSN: ISSN 0269-2139
PubMed: 2127106
DOI: 10.1093/PROTEIN/4.1.87