Calcium in PDB 1sav: Human Annexin V with Proline Substitution By Thioproline

The structure of Human Annexin V with Proline Substitution By Thioproline, PDB code: 1sav was solved by F.J.Medrano, C.Minks, N.Budisa, R.Huber, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 2.50
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	99.600, 99.600, 97.040, 90.00, 90.00, 120.00
R / Rfree (%)	18.6 / 25.9

The binding sites of Calcium atom in the Human Annexin V with Proline Substitution By Thioproline (pdb code 1sav). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 5 binding sites of Calcium where determined in the Human Annexin V with Proline Substitution By Thioproline, PDB code: 1sav:
Jump to Calcium binding site number: 1; 2; 3; 4; 5;

Calcium binding site 1 out of 5 in the Human Annexin V with Proline Substitution By Thioproline


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Human Annexin V with Proline Substitution By Thioproline within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca321 b:20.9 occ:1.00 O A:GLY102 2.0 30.4 1.0 OD2 A:ASP144 2.3 44.5 1.0 O A:GLY104 2.4 31.6 1.0 O A:LEU100 2.9 28.4 1.0 CG A:ASP144 3.1 41.4 1.0 C A:GLY102 3.2 30.7 1.0 OD1 A:ASP144 3.2 42.5 1.0 C A:GLY104 3.6 29.9 1.0 N A:GLY104 3.7 29.0 1.0 OG1 A:THR105 3.9 27.0 1.0 C A:ALA103 3.9 29.2 1.0 CA A:ALA103 4.0 29.3 1.0 N A:ALA103 4.0 29.8 1.0 C A:LEU100 4.1 26.1 1.0 N A:GLY102 4.2 30.9 1.0 CA A:GLY102 4.3 29.8 1.0 CA A:GLY104 4.3 29.4 1.0 CB A:ASP144 4.4 35.3 1.0 C A:LYS101 4.5 30.7 1.0 N A:THR105 4.6 29.9 1.0 O A:ALA103 4.7 28.6 1.0 CA A:THR105 4.8 28.2 1.0 O A:LYS101 4.8 31.3 1.0 CB A:THR105 4.9 26.2 1.0 CA A:LEU100 5.0 24.5 1.0

Calcium binding site 2 out of 5 in the Human Annexin V with Proline Substitution By Thioproline


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Human Annexin V with Proline Substitution By Thioproline within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca322 b:23.2 occ:1.00 O A:GLY263 2.5 27.0 1.0 O A:MET259 2.6 32.5 1.0 OD1 A:ASP303 2.8 39.7 1.0 OD2 A:ASP303 3.3 38.6 1.0 CG A:ASP303 3.4 37.5 1.0 O A:GLY261 3.5 38.4 1.0 C A:MET259 3.5 32.4 1.0 C A:GLY263 3.5 27.1 1.0 OG1 A:THR264 3.6 23.4 1.0 C A:GLY261 4.1 36.2 1.0 CA A:MET259 4.2 30.3 1.0 N A:GLY261 4.2 36.9 1.0 N A:GLY263 4.3 33.7 1.0 CA A:GLY263 4.3 28.4 1.0 C A:ALA262 4.4 34.7 1.0 N A:THR264 4.4 26.6 1.0 O A:ALA262 4.5 38.4 1.0 N A:LYS260 4.5 35.4 1.0 CA A:THR264 4.6 23.7 1.0 C A:LYS260 4.6 37.9 1.0 CB A:THR264 4.6 21.9 1.0 CA A:GLY261 4.7 37.0 1.0 N A:ALA262 4.8 35.8 1.0 CA A:LYS260 4.8 38.0 1.0 CB A:MET259 4.8 28.5 1.0 CB A:ASP303 4.9 35.4 1.0

Calcium binding site 3 out of 5 in the Human Annexin V with Proline Substitution By Thioproline


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Human Annexin V with Proline Substitution By Thioproline within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca323 b:24.7 occ:1.00 OE1 A:GLU72 2.1 28.9 1.0 O A:MET28 2.6 25.5 1.0 O A:GLY30 2.7 45.5 1.0 O A:HOH402 2.9 43.5 1.0 O A:GLY32 3.0 44.8 1.0 CD A:GLU72 3.0 28.8 1.0 OE2 A:GLU72 3.2 30.3 1.0 OG1 A:THR33 3.2 29.2 1.0 C A:MET28 3.7 24.3 1.0 C A:GLY32 3.8 42.4 1.0 C A:GLY30 3.9 44.0 1.0 N A:GLY32 4.2 46.5 1.0 CB A:THR33 4.3 33.5 1.0 CG A:GLU72 4.4 30.7 1.0 CA A:THR33 4.4 34.4 1.0 N A:THR33 4.4 38.5 1.0 CA A:MET28 4.4 21.9 1.0 C A:LEU31 4.5 46.9 1.0 C A:LYS29 4.5 35.7 1.0 N A:GLY30 4.6 38.3 1.0 CA A:GLY32 4.6 44.4 1.0 O A:LYS29 4.7 34.7 1.0 N A:LYS29 4.7 28.6 1.0 CA A:GLY30 4.8 41.7 1.0 N A:LEU31 4.8 46.0 1.0 CA A:LEU31 4.8 46.4 1.0 CG2 A:THR33 4.8 29.5 1.0

Calcium binding site 4 out of 5 in the Human Annexin V with Proline Substitution By Thioproline


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Human Annexin V with Proline Substitution By Thioproline within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca324 b:26.0 occ:1.00 O A:HOH457 2.9 33.6 1.0 O A:THR33 3.5 38.0 1.0 OE1 A:GLU35 3.5 27.5 1.0 OE2 A:GLU35 3.7 26.2 1.0 CD A:GLU35 4.1 25.9 1.0 N A:THR33 4.3 38.5 1.0 O A:HOH458 4.6 61.7 1.0 C A:THR33 4.6 34.8 1.0 CA A:THR33 5.0 34.4 1.0

Calcium binding site 5 out of 5 in the Human Annexin V with Proline Substitution By Thioproline


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Human Annexin V with Proline Substitution By Thioproline within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca325 b:24.6 occ:1.00 O A:LEU73 3.0 29.9 1.0 O A:LYS70 3.2 25.7 1.0 O A:SER71 3.3 30.7 1.0 O A:HOH474 3.3 56.3 1.0 C A:SER71 3.8 25.3 1.0 C A:LEU73 4.1 27.1 1.0 CA A:SER71 4.1 25.0 1.0 O A:HOH476 4.2 58.0 1.0 C A:LYS70 4.3 22.8 1.0 CB A:THR74 4.4 30.0 1.0 N A:GLU72 4.5 25.8 1.0 C A:GLU72 4.6 27.6 1.0 O A:GLU72 4.7 31.0 1.0 N A:LEU73 4.7 27.7 1.0 N A:SER71 4.8 23.3 1.0 CA A:THR74 4.8 27.5 1.0 N A:THR74 4.9 27.1 1.0 CA A:GLU72 5.0 26.6 1.0 OG1 A:THR74 5.0 34.0 1.0 OE1 A:GLU78 5.0 33.8 1.0

R.Huber, R.Berendes, A.Burger, M.Schneider, A.Karshikov, H.Luecke, J.Romisch, E.Paques. Crystal and Molecular Structure of Human Annexin V After Refinement. Implications For Structure, Membrane Binding and Ion Channel Formation of the Annexin Family of Proteins. J.Mol.Biol. V. 223 683 1992.
ISSN: ISSN 0022-2836
PubMed: 1311770
DOI: 10.1016/0022-2836(92)90984-R