Calcium in PDB 1siu: Kumamolisin-As E78H Mutant

The structure of Kumamolisin-As E78H Mutant, PDB code: 1siu was solved by M.Li, A.Wlodawer, A.Gustchina, N.Tsuruoka, M.Ashida, H.Minakata, H.Oyama, K.Oda, T.Nishino, T.Nakayama, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.31
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	42.160, 45.040, 49.430, 114.82, 106.36, 102.03
R / Rfree (%)	16.2 / 28.7

The binding sites of Calcium atom in the Kumamolisin-As E78H Mutant (pdb code 1siu). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Kumamolisin-As E78H Mutant, PDB code: 1siu:

Calcium binding site 1 out of 1 in the Kumamolisin-As E78H Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Kumamolisin-As E78H Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:27.5 occ:1.00 O A:GLY334 2.3 21.6 1.0 OD1 A:ASP316 2.3 15.5 1.0 O A:ILE317 2.3 25.9 1.0 O A:GLY336 2.3 26.0 1.0 O A:HOH501 2.3 29.4 1.0 OD2 A:ASP338 2.3 28.2 1.0 C A:ILE317 3.3 13.6 1.0 CG A:ASP316 3.3 19.4 1.0 C A:GLY334 3.5 26.2 1.0 CG A:ASP338 3.5 22.6 1.0 C A:GLY336 3.5 18.2 1.0 N A:ILE317 3.6 19.5 1.0 OD2 A:ASP316 3.8 43.8 1.0 N A:GLY336 3.8 17.6 1.0 N A:GLY334 4.0 22.4 1.0 C A:PRO335 4.0 22.5 1.0 CA A:ILE317 4.1 11.3 1.0 CA A:GLY336 4.2 11.9 1.0 N A:THR318 4.2 14.0 1.0 O A:GLY342 4.2 29.1 1.0 OG1 A:THR341 4.2 16.5 1.0 CB A:ASP338 4.2 8.4 1.0 C A:ASP316 4.4 26.5 1.0 CA A:PRO335 4.4 25.2 1.0 CA A:GLY334 4.4 21.4 1.0 N A:PRO335 4.4 20.0 1.0 OD1 A:ASP338 4.4 27.5 1.0 O A:PRO335 4.4 16.8 1.0 CA A:THR318 4.5 21.6 1.0 N A:ASP338 4.5 28.5 1.0 N A:TRP337 4.6 16.3 1.0 CB A:ASP316 4.6 14.1 1.0 CG2 A:THR318 4.7 12.5 1.0 C A:TRP337 4.7 21.0 1.0 CA A:ASP316 4.7 24.0 1.0 CB A:ILE317 4.8 19.9 1.0 CA A:TRP337 4.9 14.6 1.0

A.Wlodawer, M.Li, A.Gustchina, N.Tsuruoka, M.Ashida, H.Minakata, H.Oyama, K.Oda, T.Nishino, T.Nakayama. Crystallographic and Biochemical Investigations of Kumamolisin-As, A Serine-Carboxyl Peptidase with Collagenase Activity J.Biol.Chem. V. 279 21500 2004.
ISSN: ISSN 0021-9258
PubMed: 15014068
DOI: 10.1074/JBC.M401141200