Calcium in PDB 1sub: Calcium-Independent Subtilisin By Design

All present enzymatic activity of Calcium-Independent Subtilisin By Design:
3.4.21.62;

The structure of Calcium-Independent Subtilisin By Design, PDB code: 1sub was solved by T.Gallagher, P.Bryan, G.L.Gilliland, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.75
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	41.770, 79.520, 37.220, 90.00, 114.97, 90.00
R / Rfree (%)	n/a / n/a

The structure of Calcium-Independent Subtilisin By Design also contains other interesting chemical elements:

Potassium

(K)

1 atom

The binding sites of Calcium atom in the Calcium-Independent Subtilisin By Design (pdb code 1sub). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Calcium-Independent Subtilisin By Design, PDB code: 1sub:

Calcium binding site 1 out of 1 in the Calcium-Independent Subtilisin By Design


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Calcium-Independent Subtilisin By Design within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca295 b:6.1 occ:1.00 O A:ILE79 2.3 8.7 1.0 O A:LEU75 2.4 6.9 1.0 OD1 A:ASP41 2.4 9.0 1.0 O A:VAL81 2.4 7.8 1.0 OE1 A:GLN2 2.4 9.5 1.0 OD1 A:ASN77 2.4 8.4 1.0 OD2 A:ASP41 2.5 4.7 1.0 CG A:ASP41 2.8 7.6 1.0 CG A:ASN77 3.3 10.0 1.0 CD A:GLN2 3.4 8.5 1.0 C A:ILE79 3.5 8.6 1.0 C A:LEU75 3.5 7.3 1.0 C A:VAL81 3.6 8.0 1.0 N A:VAL81 3.7 7.4 1.0 ND2 A:ASN77 3.8 10.3 1.0 CG A:GLN2 3.9 9.0 1.0 N A:ASN77 4.1 11.0 1.0 C A:GLY80 4.1 8.0 1.0 CA A:GLY80 4.3 7.4 1.0 CB A:ASP41 4.3 6.5 1.0 CA A:VAL81 4.3 7.2 1.0 N A:LEU75 4.3 5.9 1.0 N A:GLY80 4.3 7.9 1.0 N A:ILE79 4.4 10.1 1.0 CA A:LEU75 4.4 6.6 1.0 CG1 A:ILE79 4.4 9.9 1.0 CA A:ILE79 4.5 9.8 1.0 N A:ASN76 4.5 7.5 1.0 CB A:ASN77 4.6 10.8 1.0 NE2 A:GLN2 4.6 8.5 1.0 N A:LEU82 4.6 6.7 1.0 O A:ASN77 4.6 11.0 1.0 C A:ASN77 4.7 10.4 1.0 CA A:ASN77 4.7 10.6 1.0 CA A:ASN76 4.7 10.0 1.0 CB A:GLN2 4.7 11.8 1.0 CB A:LEU75 4.7 7.9 1.0 C A:ASN76 4.8 10.6 1.0 CA A:LEU82 4.8 6.0 1.0 C A:ALA74 4.9 6.4 1.0 O A:GLY80 4.9 8.7 1.0

T.Gallagher, P.Bryan, G.L.Gilliland. Calcium-Independent Subtilisin By Design. Proteins V. 16 205 1993.
ISSN: ISSN 0887-3585
PubMed: 8332608
DOI: 10.1002/PROT.340160207