Calcium in PDB 1svn: Savinase

All present enzymatic activity of Savinase:
3.4.21.62;

The structure of Savinase, PDB code: 1svn was solved by C.Betzel, S.Klupsch, G.Papendorf, S.Hastrup, S.Branner, K.S.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.40
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	40.470, 64.240, 42.890, 90.00, 118.80, 90.00
R / Rfree (%)	17.4 / n/a

The binding sites of Calcium atom in the Savinase (pdb code 1svn). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Savinase, PDB code: 1svn:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Savinase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Savinase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca276 b:8.3 occ:1.00 O A:LEU75 2.3 5.2 1.0 O A:ILE79 2.3 7.7 1.0 ND2 A:ASN77 2.4 5.7 1.0 O A:VAL81 2.4 8.2 1.0 OD1 A:ASP41 2.5 9.6 1.0 OE1 A:GLN2 2.5 8.1 1.0 OD2 A:ASP41 2.5 8.8 1.0 CG A:ASP41 2.9 9.2 1.0 CG A:ASN77 3.4 9.9 1.0 C A:LEU75 3.4 4.3 1.0 CD A:GLN2 3.5 9.1 1.0 C A:ILE79 3.5 8.7 1.0 C A:VAL81 3.6 6.4 1.0 N A:VAL81 3.6 6.0 1.0 OD1 A:ASN77 3.9 15.7 1.0 N A:ASN77 4.0 8.6 1.0 CG A:GLN2 4.0 8.0 1.0 C A:GLY80 4.1 8.1 1.0 CA A:VAL81 4.2 7.1 1.0 N A:LEU75 4.3 6.5 1.0 CA A:LEU75 4.3 6.4 1.0 CG1 A:ILE79 4.3 10.5 1.0 CA A:GLY80 4.3 7.5 1.0 CB A:ASP41 4.3 8.2 1.0 N A:ILE79 4.4 11.7 1.0 N A:GLY80 4.4 10.1 1.0 N A:ASN76 4.4 8.5 1.0 N A:LEU82 4.6 5.3 1.0 CA A:ASN76 4.6 10.2 1.0 CA A:ILE79 4.6 8.9 1.0 CB A:LEU75 4.6 7.2 1.0 CB A:GLN2 4.6 8.1 1.0 NE2 A:GLN2 4.6 9.6 1.0 CB A:ASN77 4.6 8.9 1.0 C A:ASN76 4.7 10.0 1.0 C A:ALA74 4.7 6.5 1.0 CA A:ASN77 4.7 10.6 1.0 CA A:LEU82 4.8 5.7 1.0 C A:ASN77 4.8 10.1 1.0 O A:ASN77 4.8 13.4 1.0 O A:GLY80 4.8 9.2 1.0 CB A:ALA74 5.0 5.8 1.0

Calcium binding site 2 out of 2 in the Savinase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Savinase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca277 b:14.2 occ:0.60 O A:HOH287 2.2 9.0 1.0 O A:ALA169 2.3 7.2 1.0 O A:ALA174 2.4 7.0 1.0 O A:TYR171 2.5 8.2 1.0 O A:HOH315 2.5 16.2 1.0 C A:TYR171 3.4 6.8 1.0 C A:ALA169 3.5 8.1 1.0 C A:ALA174 3.5 7.3 1.0 O A:ALA172 3.8 10.7 1.0 C A:ARG170 3.8 7.2 1.0 O A:ARG170 3.9 10.6 1.0 N A:ALA174 4.0 6.4 1.0 N A:TYR171 4.0 5.8 1.0 C A:ALA172 4.0 9.4 1.0 CA A:ALA172 4.0 6.7 1.0 N A:ALA172 4.0 7.3 1.0 O A:GLY195 4.1 11.4 1.0 CA A:ALA174 4.2 6.2 1.0 CB A:ALA176 4.2 5.4 1.0 CA A:ARG170 4.3 10.0 1.0 CB A:ALA174 4.3 6.9 1.0 CA A:TYR171 4.3 7.5 1.0 N A:ARG170 4.3 7.5 1.0 N A:ALA176 4.3 6.0 1.0 NH2 A:ARG247 4.3 14.8 1.0 OD2 A:ASP197 4.4 11.1 1.0 CA A:ALA169 4.4 8.4 1.0 N A:MET175 4.5 7.7 1.0 CA A:MET175 4.7 5.9 1.0 C A:MET175 4.8 4.7 1.0 O A:PRO168 4.8 7.0 1.0 N A:ASN173 4.8 7.8 1.0 CA A:ALA176 4.9 5.2 1.0 C A:GLY195 5.0 11.2 1.0

C.Betzel, S.Klupsch, G.Papendorf, S.Hastrup, S.Branner, K.S.Wilson. Crystal Structure of the Alkaline Proteinase Savinase From Bacillus Lentus at 1.4 A Resolution. J.Mol.Biol. V. 223 427 1992.
ISSN: ISSN 0022-2836
PubMed: 1738156
DOI: 10.1016/0022-2836(92)90662-4