Calcium in PDB 1trq: X-Ray Crystallographic and Calorimeric Studies of the Effects of the Mutation Trp 59 Tyr in Ribonuclease T1

All present enzymatic activity of X-Ray Crystallographic and Calorimeric Studies of the Effects of the Mutation Trp 59 Tyr in Ribonuclease T1:
3.1.27.3;

The structure of X-Ray Crystallographic and Calorimeric Studies of the Effects of the Mutation Trp 59 Tyr in Ribonuclease T1, PDB code: 1trq was solved by W.-D.Schubert, W.Saenger, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.30
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	49.340, 48.200, 40.150, 90.00, 90.29, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Calcium atom in the X-Ray Crystallographic and Calorimeric Studies of the Effects of the Mutation Trp 59 Tyr in Ribonuclease T1 (pdb code 1trq). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the X-Ray Crystallographic and Calorimeric Studies of the Effects of the Mutation Trp 59 Tyr in Ribonuclease T1, PDB code: 1trq:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the X-Ray Crystallographic and Calorimeric Studies of the Effects of the Mutation Trp 59 Tyr in Ribonuclease T1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of X-Ray Crystallographic and Calorimeric Studies of the Effects of the Mutation Trp 59 Tyr in Ribonuclease T1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca106 b:36.9 occ:1.00 O A:HOH468 2.3 40.0 1.0 OD2 A:ASP15 2.4 27.5 1.0 O A:HOH499 2.5 51.6 1.0 OD1 A:ASP15 2.8 34.6 1.0 CG A:ASP15 2.9 25.0 1.0 O A:HOH506 3.2 53.5 1.0 CB A:ASP15 4.4 20.6 1.0 O A:HOH471 4.5 40.6 1.0 O A:CYS10 4.6 29.8 1.0 N A:SER12 4.8 30.9 1.0 O A:HOH448 4.9 36.0 1.0 OG A:SER12 5.0 31.1 1.0

Calcium binding site 2 out of 2 in the X-Ray Crystallographic and Calorimeric Studies of the Effects of the Mutation Trp 59 Tyr in Ribonuclease T1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of X-Ray Crystallographic and Calorimeric Studies of the Effects of the Mutation Trp 59 Tyr in Ribonuclease T1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca306 b:45.1 occ:1.00 OD2 B:ASP215 2.1 22.3 1.0 O B:HOH444 2.4 34.6 1.0 O B:HOH414 2.7 27.2 1.0 O B:HOH479 2.7 44.0 1.0 CG B:ASP215 2.8 5.0 1.0 O B:HOH456 2.8 38.2 1.0 OD1 B:ASP215 2.8 46.3 1.0 O B:HOH487 3.2 47.5 1.0 CB B:ASP215 4.2 10.9 1.0 O B:CYS210 4.6 26.3 1.0 OG B:SER212 4.6 11.3 1.0 O B:HOH406 4.6 24.5 1.0 N B:SER212 4.8 43.0 1.0 O B:HOH413 4.8 26.8 1.0 CB B:SER212 4.9 17.5 1.0

W.D.Schubert, G.Schluckebier, J.Backmann, J.Granzin, C.Kisker, H.W.Choe, U.Hahn, W.Pfeil, W.Saenger. X-Ray Crystallographic and Calorimetric Studies of the Effects of the Mutation TRP59-->Tyr in Ribonuclease T1. Eur.J.Biochem. V. 220 527 1994.
ISSN: ISSN 0014-2956
PubMed: 8125111
DOI: 10.1111/J.1432-1033.1994.TB18652.X