Atomistry » Calcium » PDB 1tu5-1ujc » 1ua7
Atomistry »
  Calcium »
    PDB 1tu5-1ujc »
      1ua7 »

Calcium in PDB 1ua7: Crystal Structure Analysis of Alpha-Amylase From Bacillus Subtilis Complexed with Acarbose

Enzymatic activity of Crystal Structure Analysis of Alpha-Amylase From Bacillus Subtilis Complexed with Acarbose

All present enzymatic activity of Crystal Structure Analysis of Alpha-Amylase From Bacillus Subtilis Complexed with Acarbose:
3.2.1.1;

Protein crystallography data

The structure of Crystal Structure Analysis of Alpha-Amylase From Bacillus Subtilis Complexed with Acarbose, PDB code: 1ua7 was solved by M.Kagawa, Z.Fujimoto, M.Momma, K.Takase, H.Mizuno, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.97 / 2.21
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 70.320, 74.204, 115.724, 90.00, 90.00, 90.00
R / Rfree (%) 20.8 / 26.5

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure Analysis of Alpha-Amylase From Bacillus Subtilis Complexed with Acarbose (pdb code 1ua7). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Crystal Structure Analysis of Alpha-Amylase From Bacillus Subtilis Complexed with Acarbose, PDB code: 1ua7:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in 1ua7

Go back to Calcium Binding Sites List in 1ua7
Calcium binding site 1 out of 3 in the Crystal Structure Analysis of Alpha-Amylase From Bacillus Subtilis Complexed with Acarbose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure Analysis of Alpha-Amylase From Bacillus Subtilis Complexed with Acarbose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca601

b:13.9
occ:1.00
O A:HIS180 2.2 15.2 1.0
O A:HOH667 2.3 27.6 1.0
O A:HOH608 2.4 7.0 1.0
O A:THR137 2.4 19.9 1.0
O A:HOH798 2.5 10.3 1.0
OD1 A:ASN101 2.5 11.8 1.0
OD1 A:ASP146 2.6 23.3 1.0
OD2 A:ASP146 2.6 20.2 1.0
CG A:ASP146 2.9 18.0 1.0
C A:HIS180 3.4 16.5 1.0
C A:THR137 3.5 20.3 1.0
CG A:ASN101 3.7 14.3 1.0
CA A:THR137 4.1 18.9 1.0
CB A:HIS180 4.2 14.3 1.0
ND2 A:ASN101 4.2 6.9 1.0
CA A:ILE181 4.3 16.1 1.0
N A:ILE181 4.3 16.3 1.0
CA A:HIS180 4.4 16.4 1.0
O A:ASN101 4.4 14.8 1.0
O A:ASN139 4.4 21.8 1.0
CG1 A:ILE181 4.4 12.5 1.0
CB A:ASP146 4.4 18.8 1.0
ND1 A:HIS122 4.5 18.3 1.0
O A:TRP147 4.6 20.3 1.0
N A:GLN138 4.6 21.4 1.0
CE1 A:HIS122 4.8 20.8 1.0
O A:VAL136 4.8 18.0 1.0
O A:HOH648 4.8 16.9 1.0
OE1 A:GLU182 4.8 12.6 1.0
CA A:GLN138 4.9 21.1 1.0
CB A:THR137 4.9 17.6 1.0
CB A:ASN101 4.9 11.9 1.0
CB A:ILE181 5.0 14.8 1.0

Calcium binding site 2 out of 3 in 1ua7

Go back to Calcium Binding Sites List in 1ua7
Calcium binding site 2 out of 3 in the Crystal Structure Analysis of Alpha-Amylase From Bacillus Subtilis Complexed with Acarbose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure Analysis of Alpha-Amylase From Bacillus Subtilis Complexed with Acarbose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca602

b:19.1
occ:1.00
O A:GLY169 2.3 18.8 1.0
OD2 A:ASP171 2.3 12.9 1.0
OD1 A:ASP171 2.3 16.0 1.0
O A:HOH800 2.4 17.1 1.0
O A:HOH796 2.4 15.8 1.0
O A:HOH801 2.4 9.3 1.0
CG A:ASP171 2.6 16.9 1.0
O A:HOH716 2.6 14.9 1.0
C A:GLY169 3.4 16.9 1.0
CA A:GLY169 4.0 18.3 1.0
CB A:ASP171 4.1 15.5 1.0
C A:ALA170 4.2 15.1 1.0
O A:ALA170 4.2 15.8 1.0
O A:HOH809 4.4 15.7 1.0
N A:ASP171 4.4 16.0 1.0
N A:ALA170 4.4 13.1 1.0
OE1 A:GLU88 4.5 25.0 1.0
O A:HOH821 4.5 24.9 1.0
O A:HOH678 4.6 15.6 1.0
CA A:ALA170 4.7 14.6 1.0
CA A:ASP171 4.7 13.6 1.0
O A:CYS84 4.7 11.5 1.0
N A:VAL94 4.8 19.0 1.0
CB A:CYS84 4.9 16.9 1.0
O A:HOH772 4.9 27.7 1.0

Calcium binding site 3 out of 3 in 1ua7

Go back to Calcium Binding Sites List in 1ua7
Calcium binding site 3 out of 3 in the Crystal Structure Analysis of Alpha-Amylase From Bacillus Subtilis Complexed with Acarbose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure Analysis of Alpha-Amylase From Bacillus Subtilis Complexed with Acarbose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca603

b:11.8
occ:1.00
O A:HOH905 2.3 22.9 1.0
O A:GLY313 2.3 13.7 1.0
O A:HOH795 2.4 10.0 1.0
OE2 A:GLU276 2.4 11.4 1.0
O A:HOH811 2.5 16.8 1.0
OE1 A:GLU276 2.5 10.6 1.0
CD A:GLU276 2.8 11.6 1.0
C A:GLY313 3.5 13.9 1.0
CA A:VAL314 4.1 15.2 1.0
O A:HOH1012 4.2 34.3 1.0
O A:HOH783 4.2 23.4 1.0
N A:ARG315 4.2 13.8 1.0
N A:VAL314 4.2 13.4 1.0
O A:HOH640 4.3 6.4 1.0
CG A:GLU276 4.3 12.3 1.0
CA A:GLY313 4.6 11.3 1.0
O A:HOH951 4.7 29.9 1.0
C A:VAL314 4.7 15.5 1.0
O A:HOH868 4.9 28.4 1.0

Reference:

M.Kagawa, Z.Fujimoto, M.Momma, K.Takase, H.Mizuno. Crystal Structure of Bacillus Subtilis Alpha-Amylase in Complex with Acarbose J.Bacteriol. V. 185 6981 2003.
ISSN: ISSN 0021-9193
PubMed: 14617662
DOI: 10.1128/JB.185.23.6981-6984.2003
Page generated: Sat Dec 12 03:22:41 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy