Calcium in PDB 1uqx: Ralstonia Solanacearum Lectin (Rs-Iil) in Complex with Alpha- Methylmannoside

The structure of Ralstonia Solanacearum Lectin (Rs-Iil) in Complex with Alpha- Methylmannoside, PDB code: 1uqx was solved by E.P.Mitchell, M.Wimmerova, A.Imberty, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.17 / 1.70
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	59.132, 61.707, 74.788, 90.00, 90.00, 90.00
R / Rfree (%)	11.6 / 13.9

The binding sites of Calcium atom in the Ralstonia Solanacearum Lectin (Rs-Iil) in Complex with Alpha- Methylmannoside (pdb code 1uqx). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Ralstonia Solanacearum Lectin (Rs-Iil) in Complex with Alpha- Methylmannoside, PDB code: 1uqx:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Ralstonia Solanacearum Lectin (Rs-Iil) in Complex with Alpha- Methylmannoside


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ralstonia Solanacearum Lectin (Rs-Iil) in Complex with Alpha- Methylmannoside within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1114 b:8.0 occ:1.00 O A:ASN21 2.3 7.6 1.0 OD1 A:ASP103 2.4 7.6 1.0 OD1 A:ASN102 2.4 6.2 1.0 OD2 A:ASP100 2.4 8.6 1.0 O3 A:MMA1116 2.5 8.0 1.0 O2 A:MMA1116 2.5 7.8 1.0 OD1 A:ASP100 3.1 7.1 1.0 CG A:ASP100 3.1 8.3 1.0 C2 A:MMA1116 3.4 6.0 1.0 C3 A:MMA1116 3.4 7.0 1.0 CG A:ASN102 3.4 8.4 1.0 C A:ASN21 3.5 7.9 1.0 CG A:ASP103 3.6 7.3 1.0 CA A:CA1115 3.8 6.9 1.0 ND2 A:ASN102 3.8 7.4 1.0 C4 A:MMA1116 3.9 6.4 1.0 CB A:ASN21 4.2 7.1 1.0 CA A:ASP103 4.2 6.7 1.0 N A:ASP103 4.3 6.8 1.0 N A:ALA22 4.4 8.6 1.0 CB A:ASP103 4.4 6.5 1.0 OD2 A:ASP103 4.4 8.7 1.0 CA A:ALA22 4.4 8.5 1.0 CA A:ASN21 4.5 7.2 1.0 C A:ASN102 4.5 7.1 1.0 OD2 A:ASP98 4.5 9.2 1.0 O4 A:MMA1116 4.5 7.4 1.0 CB A:ASP100 4.5 10.1 1.0 O A:HOH2172 4.5 15.7 1.0 CB A:ASN102 4.7 8.0 1.0 C1 A:MMA1116 4.8 8.0 1.0 O A:ASN102 4.8 7.6 1.0 N A:ASN102 4.9 8.0 1.0 CA A:ASN102 4.9 8.3 1.0 CB A:ALA22 5.0 8.5 1.0 OD1 A:ASP98 5.0 9.4 1.0

Calcium binding site 2 out of 2 in the Ralstonia Solanacearum Lectin (Rs-Iil) in Complex with Alpha- Methylmannoside


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Ralstonia Solanacearum Lectin (Rs-Iil) in Complex with Alpha- Methylmannoside within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1115 b:6.9 occ:1.00 OD1 A:ASP98 2.4 9.4 1.0 OD1 A:ASP100 2.4 7.1 1.0 OE2 A:GLU94 2.4 9.0 1.0 OD2 A:ASP103 2.4 8.7 1.0 OE1 A:GLU94 2.5 7.9 1.0 O3 A:MMA1116 2.5 8.0 1.0 O4 A:MMA1116 2.5 7.4 1.0 OD1 A:ASP103 2.6 7.6 1.0 CD A:GLU94 2.8 8.9 1.0 CG A:ASP103 2.9 7.3 1.0 C4 A:MMA1116 3.4 6.4 1.0 C3 A:MMA1116 3.4 7.0 1.0 CG A:ASP98 3.4 9.9 1.0 CG A:ASP100 3.4 8.3 1.0 OD2 A:ASP98 3.7 9.2 1.0 CA A:CA1114 3.8 8.0 1.0 OD2 A:ASP100 4.0 8.6 1.0 O A:HOH2177 4.1 9.3 1.0 OD1 A:ASP95 4.1 8.8 1.0 N A:ASP100 4.2 9.9 1.0 CG A:GLU94 4.3 7.9 1.0 CB A:ASP103 4.4 6.5 1.0 N A:ASN99 4.5 10.8 1.0 N A:TYR101 4.5 9.2 1.0 N A:ASP95 4.5 8.5 1.0 CB A:ASP100 4.6 10.1 1.0 CA A:GLU94 4.7 7.9 1.0 C2 A:MMA1116 4.7 6.0 1.0 N A:GLY96 4.7 9.2 1.0 CA A:ASP100 4.7 9.6 1.0 CB A:ASP98 4.8 11.3 1.0 OD1 A:ASN102 4.8 6.2 1.0 N A:ASP98 4.8 10.9 1.0 C5 A:MMA1116 4.9 2.0 1.0 O2 A:MMA1116 4.9 7.8 1.0 CB A:GLU94 4.9 8.0 1.0 CG A:ASP95 5.0 8.4 1.0 C A:ASP98 5.0 10.9 1.0

D.Sudakevitz, N.Kostlanova, G.Blatman-Jan, E.P.Mitchell, B.Lerrer, M.Wimmerova, D.J.Katcoff, A.Imberty, N.Gilboa-Garber. A New Ralstonia Solanacearum High-Affinity Mannose-Binding Lectin Rs-Iil Structurally Resembling the Pseudomonas Aeruginosa Fucose-Specific Lectin Pa-Iil Mol.Microbiol. V. 52 691 2004.
ISSN: ISSN 0950-382X
PubMed: 15101976
DOI: 10.1111/J.1365-2958.2004.04020.X