Calcium in PDB 1uv4: Native Bacillus Subtilis Arabinanase ARB43A

All present enzymatic activity of Native Bacillus Subtilis Arabinanase ARB43A:
3.2.1.99;

The structure of Native Bacillus Subtilis Arabinanase ARB43A, PDB code: 1uv4 was solved by D.Nurizzo, E.J.Taylor, H.J.Gilbert, G.J.Davies, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.50
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	48.628, 46.153, 57.322, 90.00, 98.96, 90.00
R / Rfree (%)	16.7 / 19.9

The binding sites of Calcium atom in the Native Bacillus Subtilis Arabinanase ARB43A (pdb code 1uv4). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Native Bacillus Subtilis Arabinanase ARB43A, PDB code: 1uv4:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Native Bacillus Subtilis Arabinanase ARB43A


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Native Bacillus Subtilis Arabinanase ARB43A within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca502 b:12.5 occ:1.00 O A:HOH2160 2.3 17.6 1.0 O A:HOH2014 2.3 14.7 1.0 O A:HOH2008 2.4 14.3 1.0 O A:HOH2110 2.4 15.5 1.0 O A:HOH2275 2.5 11.3 1.0 O A:HOH2204 2.5 17.3 1.0 O A:HOH2108 2.6 17.2 1.0 O A:HOH2203 2.6 17.9 1.0 O A:PRO134 4.2 13.6 1.0 O A:PRO76 4.4 13.9 1.0 O A:PRO187 4.4 11.9 1.0 O A:GLN256 4.5 13.3 1.0 O A:PRO15 4.6 15.0 1.0 O A:GLY255 4.8 13.9 1.0 O A:ALA75 4.8 14.6 1.0 CB A:ALA186 4.8 14.9 1.0 CB A:ALA75 4.8 13.1 1.0 O A:HOH2013 4.9 24.0 1.0 CA A:ALA186 4.9 13.3 1.0 OD1 A:ASP77 4.9 15.3 1.0 O A:ASP133 5.0 13.7 1.0 O A:ASP14 5.0 12.9 1.0

Calcium binding site 2 out of 2 in the Native Bacillus Subtilis Arabinanase ARB43A


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Native Bacillus Subtilis Arabinanase ARB43A within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1294 b:13.8 occ:1.00 OD1 A:ASP77 2.3 15.3 1.0 OD1 A:ASP257 2.3 20.4 1.0 O A:HOH2300 2.3 15.0 0.5 O A:HOH2161 2.3 12.9 1.0 OE1 A:GLU135 2.4 16.8 1.0 O A:HOH2111 2.4 14.6 1.0 OD2 A:ASP257 2.6 16.0 1.0 O A:HOH2299 2.7 13.5 0.5 CG A:ASP257 2.9 16.1 1.0 CG A:ASP77 3.3 14.3 1.0 CD A:GLU135 3.4 16.6 1.0 OD2 A:ASP77 3.6 14.3 1.0 OE2 A:GLU135 3.7 16.9 1.0 O A:HOH2203 4.1 17.9 1.0 O A:HOH2110 4.1 15.5 1.0 OG1 A:THR16 4.2 18.3 1.0 OE1 A:GLN79 4.2 19.8 1.0 O A:ILE258 4.3 15.6 1.0 CB A:ASP257 4.3 12.8 1.0 O1 A:EDO1297 4.5 28.5 0.5 O A:LEU136 4.5 13.9 1.0 OG1 A:THR188 4.6 14.6 1.0 CB A:ASP77 4.6 14.4 1.0 O A:ILE78 4.8 14.5 1.0 CG A:GLU135 4.8 16.5 1.0 O A:HOH2205 4.8 29.6 1.0 N A:ILE258 5.0 15.3 1.0

M.Proctor, E.J.Taylor, D.Nurizzo, J.Turkenburg, R.Lloyd, M.Vardakou, G.J.Davies, H.J.Gilbert. Tailored Catalysts For Plant Cell-Wall Degradation: Redesigning the Exo/Endo Preference of Cellvibrio Japonicus Arabinanase 43A Proc.Natl.Acad.Sci.Usa V. 102 2697 2005.
ISSN: ISSN 0027-8424
PubMed: 15708971
DOI: 10.1073/PNAS.0500051102