Calcium in PDB 1v3j: Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase

All present enzymatic activity of Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase:
2.4.1.19;

The structure of Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase, PDB code: 1v3j was solved by R.Kanai, K.Haga, T.Akiba, K.Yamane, K.Harata, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.00
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	63.930, 73.660, 78.200, 85.11, 104.96, 100.97
R / Rfree (%)	17 / 24.2

The binding sites of Calcium atom in the Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase (pdb code 1v3j). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase, PDB code: 1v3j:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca687 b:13.6 occ:1.00 OD1 A:ASN33 2.1 16.0 1.0 O A:ASN29 2.2 22.9 1.0 O A:HOH728 2.3 12.0 1.0 O A:GLY51 2.3 17.1 1.0 ND2 A:ASN32 2.4 16.7 1.0 OD1 A:ASP53 2.4 15.4 1.0 OD2 A:ASP27 2.5 16.1 1.0 CG A:ASN33 3.3 14.6 1.0 C A:ASN29 3.4 20.4 1.0 CG A:ASP27 3.4 14.7 1.0 C A:GLY51 3.4 12.5 1.0 CG A:ASP53 3.5 15.4 1.0 CG A:ASN32 3.5 16.1 1.0 CB A:ASP53 3.9 13.8 1.0 CA A:GLY51 4.0 10.0 1.0 O A:ALA111 4.0 20.2 1.0 OD1 A:ASP27 4.0 13.5 1.0 OD1 A:ASN32 4.0 15.0 1.0 N A:ASN33 4.1 14.1 1.0 ND2 A:ASN33 4.1 15.4 1.0 N A:PRO30 4.2 19.1 1.0 CA A:ASN33 4.3 16.6 1.0 N A:ASN29 4.3 19.7 1.0 C A:ASN32 4.3 15.3 1.0 CA A:PRO30 4.3 18.9 1.0 CA A:ASN29 4.4 19.7 1.0 CB A:ASN33 4.4 15.3 1.0 CB A:ASP27 4.4 11.7 1.0 OD2 A:ASP53 4.5 13.1 1.0 CA A:ASP27 4.5 12.8 1.0 N A:GLY52 4.5 14.2 1.0 C A:GLY52 4.6 11.4 1.0 O A:ASN32 4.6 14.4 1.0 CB A:ASN32 4.7 16.5 1.0 N A:ASN32 4.7 17.4 1.0 O A:PRO30 4.7 19.9 1.0 O A:GLY52 4.7 12.7 1.0 N A:ASP53 4.7 13.2 1.0 C A:PRO30 4.7 17.8 1.0 O A:HOH1176 4.8 15.4 1.0 CA A:ASN32 4.8 15.0 1.0 CB A:ASN29 4.8 21.8 1.0 CA A:GLY52 4.8 13.2 1.0 C A:ASP27 4.9 13.3 1.0 CA A:ASP53 5.0 11.5 1.0

Calcium binding site 2 out of 4 in the Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca688 b:20.0 occ:1.00 O A:HOH1278 2.1 16.1 1.0 O A:HOH1024 2.3 16.7 1.0 O A:HOH1279 2.3 21.2 1.0 O A:ILE190 2.3 12.7 1.0 O A:HIS233 2.4 16.9 1.0 ND2 A:ASN139 2.4 10.7 1.0 OD1 A:ASP199 2.7 18.1 1.0 OD2 A:ASP199 2.7 20.6 1.0 CG A:ASP199 3.1 18.1 1.0 C A:ILE190 3.5 13.7 1.0 CG A:ASN139 3.5 12.9 1.0 C A:HIS233 3.6 13.2 1.0 OD1 A:ASN139 3.9 17.5 1.0 CA A:ILE190 4.1 12.7 1.0 O A:ASN139 4.3 12.3 1.0 CB A:HIS233 4.5 14.6 1.0 O A:GLY189 4.5 14.9 1.0 CA A:MET234 4.5 12.1 1.0 N A:MET234 4.5 13.5 1.0 O A:LYS192 4.5 18.7 1.0 CA A:HIS233 4.5 13.9 1.0 N A:TYR191 4.6 14.6 1.0 ND1 A:HIS176 4.6 16.9 1.0 CB A:ASP199 4.6 12.2 1.0 CG A:MET234 4.6 10.1 1.0 O A:HOH1284 4.6 23.0 1.0 O A:LEU200 4.7 15.8 1.0 CG2 A:ILE190 4.7 12.6 1.0 CE1 A:HIS176 4.7 19.7 1.0 CB A:ASN139 4.8 10.8 1.0 CA A:TYR191 4.9 12.6 1.0 O A:HOH1186 5.0 13.7 1.0

Calcium binding site 3 out of 4 in the Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca689 b:18.2 occ:1.00 O B:ASN29 2.2 25.7 1.0 O B:HOH1299 2.2 21.2 1.0 O B:GLY51 2.2 22.7 1.0 ND2 B:ASN33 2.3 18.4 1.0 OD2 B:ASP27 2.3 18.6 1.0 ND2 B:ASN32 2.4 21.9 1.0 OD2 B:ASP53 2.5 17.1 1.0 C B:ASN29 3.3 22.6 1.0 CG B:ASP27 3.4 18.8 1.0 CG B:ASP53 3.4 17.7 1.0 C B:GLY51 3.4 19.2 1.0 CG B:ASN33 3.5 15.8 1.0 CG B:ASN32 3.5 20.7 1.0 OD1 B:ASN32 3.8 24.7 1.0 CB B:ASP53 3.9 15.6 1.0 O B:ALA111 4.0 15.0 1.0 OD1 B:ASN33 4.0 15.5 1.0 CA B:GLY51 4.0 18.6 1.0 OD1 B:ASP27 4.1 19.1 1.0 N B:PRO30 4.1 23.3 1.0 N B:ASN33 4.1 14.5 1.0 CA B:PRO30 4.2 25.5 1.0 N B:ASN29 4.2 22.9 1.0 CB B:ASP27 4.3 18.7 1.0 CA B:ASN29 4.3 21.7 1.0 CA B:ASN33 4.3 17.1 1.0 C B:ASN32 4.4 13.7 1.0 CA B:ASP27 4.4 18.2 1.0 OD1 B:ASP53 4.4 18.4 1.0 N B:GLY52 4.5 17.5 1.0 CB B:ASN33 4.6 14.0 1.0 O B:ASN32 4.6 15.0 1.0 C B:GLY52 4.7 16.6 1.0 CB B:ASN32 4.7 20.3 1.0 C B:PRO30 4.7 24.4 1.0 CB B:ASN29 4.7 20.8 1.0 N B:ASN32 4.8 19.3 1.0 O B:PRO30 4.8 28.1 1.0 O B:HOH710 4.8 14.4 1.0 C B:ASP27 4.8 16.5 1.0 N B:ASP53 4.8 15.2 1.0 CA B:ASN32 4.9 16.9 1.0 N B:GLY28 4.9 17.6 1.0 O B:GLY52 4.9 14.9 1.0 CA B:GLY52 4.9 17.1 1.0 CA B:ASP53 5.0 15.0 1.0

Calcium binding site 4 out of 4 in the Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of F283L Mutant Cyclodextrin Glycosyltransferase within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca690 b:21.8 occ:1.00 O B:HOH1251 2.1 18.1 1.0 O B:HOH742 2.2 17.5 1.0 O B:ILE190 2.3 13.9 1.0 O B:HIS233 2.3 16.5 1.0 ND2 B:ASN139 2.4 16.2 1.0 OD2 B:ASP199 2.6 18.9 1.0 OD1 B:ASP199 2.7 20.6 1.0 CG B:ASP199 3.0 16.9 1.0 C B:ILE190 3.5 16.6 1.0 CG B:ASN139 3.6 18.5 1.0 C B:HIS233 3.6 15.4 1.0 OD1 B:ASN139 3.9 20.1 1.0 CA B:ILE190 4.1 14.1 1.0 O B:GLY189 4.3 15.4 1.0 CB B:HIS233 4.4 15.2 1.0 O B:LYS192 4.4 19.7 1.0 N B:MET234 4.4 15.6 1.0 CB B:ASP199 4.4 15.2 1.0 CA B:MET234 4.5 14.2 1.0 CA B:HIS233 4.5 15.8 1.0 O B:ASN139 4.5 15.2 1.0 N B:TYR191 4.5 17.5 1.0 ND1 B:HIS176 4.7 20.9 1.0 O B:LEU200 4.8 18.8 1.0 CG B:MET234 4.8 12.6 1.0 CB B:ASN139 4.8 16.7 1.0 CA B:TYR191 4.9 19.7 1.0 CG2 B:ILE190 4.9 11.6 1.0 CE1 B:HIS176 5.0 19.1 1.0

R.Kanai, K.Haga, T.Akiba, K.Yamane, K.Harata. Role of PHE283 in Enzymatic Reaction of Cyclodextrin Glycosyltransferase From Alkalophilic Bacillus Sp.1011: Substrate Binding and Arrangement of the Catalytic Site Protein Sci. V. 13 457 2004.
ISSN: ISSN 0961-8368
PubMed: 14739329
DOI: 10.1110/PS.03408504