Calcium in PDB 1v6j: Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH
Protein crystallography data
The structure of Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH, PDB code: 1v6j
was solved by
S.Kundhavai Natchiar,
A.Arockia Jeyaprakash,
T.N.C.Ramya,
C.J.Thomas,
K.Suguna,
A.Surolia,
M.Vijayan,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
20.00 /
2.90
|
Space group
|
P 21 21 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
128.733,
125.531,
76.050,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
16.1 /
22
|
Other elements in 1v6j:
The structure of Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH also contains other interesting chemical elements:
Calcium Binding Sites:
The binding sites of Calcium atom in the Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH
(pdb code 1v6j). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the
Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH, PDB code: 1v6j:
Jump to Calcium binding site number:
1;
2;
3;
4;
Calcium binding site 1 out
of 4 in 1v6j
Go back to
Calcium Binding Sites List in 1v6j
Calcium binding site 1 out
of 4 in the Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca237
b:26.6
occ:1.00
|
OD1
|
A:ASP123
|
2.7
|
21.8
|
1.0
|
OD2
|
A:ASP132
|
2.7
|
36.2
|
1.0
|
O
|
A:TYR125
|
2.7
|
22.3
|
1.0
|
OD1
|
A:ASN127
|
2.8
|
30.9
|
1.0
|
OD2
|
A:ASP123
|
2.9
|
24.5
|
1.0
|
CG
|
A:ASP123
|
3.1
|
23.1
|
1.0
|
CG
|
A:ASN127
|
3.5
|
29.8
|
1.0
|
CB
|
A:ASN127
|
3.8
|
25.5
|
1.0
|
CG
|
A:ASP132
|
3.8
|
41.0
|
1.0
|
C
|
A:TYR125
|
3.9
|
19.8
|
1.0
|
CA
|
A:GLY104
|
4.0
|
24.1
|
1.0
|
N
|
A:ASN127
|
4.0
|
24.2
|
1.0
|
O
|
A:GLY104
|
4.3
|
26.0
|
1.0
|
OD1
|
A:ASP132
|
4.3
|
41.5
|
1.0
|
O
|
A:ASP83
|
4.5
|
49.4
|
1.0
|
OD1
|
A:ASP83
|
4.5
|
24.8
|
1.0
|
CA
|
A:ASN127
|
4.6
|
19.2
|
1.0
|
CB
|
A:ASP123
|
4.6
|
21.0
|
1.0
|
ND2
|
A:ASN127
|
4.6
|
29.6
|
1.0
|
C
|
A:GLY104
|
4.6
|
25.4
|
1.0
|
CA
|
A:TYR125
|
4.7
|
20.1
|
1.0
|
N
|
A:TYR125
|
4.7
|
23.2
|
1.0
|
CB
|
A:TYR125
|
4.7
|
29.2
|
1.0
|
MN
|
A:MN238
|
4.8
|
46.2
|
1.0
|
CD1
|
A:TYR125
|
4.8
|
30.3
|
1.0
|
N
|
A:SER126
|
4.8
|
21.9
|
1.0
|
C
|
A:SER126
|
4.9
|
29.0
|
1.0
|
CA
|
A:SER126
|
4.9
|
26.6
|
1.0
|
|
Calcium binding site 2 out
of 4 in 1v6j
Go back to
Calcium Binding Sites List in 1v6j
Calcium binding site 2 out
of 4 in the Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca237
b:27.3
occ:1.00
|
OD1
|
B:ASN127
|
2.6
|
26.2
|
1.0
|
OD1
|
B:ASP123
|
2.6
|
26.5
|
1.0
|
O
|
B:TYR125
|
2.7
|
14.6
|
1.0
|
OD2
|
B:ASP132
|
2.7
|
35.0
|
1.0
|
OD2
|
B:ASP123
|
2.8
|
26.7
|
1.0
|
CG
|
B:ASP123
|
3.1
|
27.3
|
1.0
|
CG
|
B:ASN127
|
3.5
|
25.6
|
1.0
|
C
|
B:TYR125
|
3.8
|
14.4
|
1.0
|
CG
|
B:ASP132
|
3.9
|
37.6
|
1.0
|
CA
|
B:GLY104
|
4.0
|
19.2
|
1.0
|
CB
|
B:ASN127
|
4.1
|
24.3
|
1.0
|
N
|
B:ASN127
|
4.3
|
30.1
|
1.0
|
O
|
B:GLY104
|
4.3
|
19.9
|
1.0
|
OD1
|
B:ASP132
|
4.3
|
38.0
|
1.0
|
O
|
B:ASP83
|
4.4
|
35.2
|
1.0
|
CB
|
B:ASP83
|
4.4
|
38.5
|
1.0
|
ND2
|
B:ASN127
|
4.4
|
24.6
|
1.0
|
CB
|
B:ASP123
|
4.6
|
27.5
|
1.0
|
C
|
B:GLY104
|
4.6
|
22.5
|
1.0
|
CA
|
B:TYR125
|
4.7
|
15.1
|
1.0
|
CB
|
B:TYR125
|
4.7
|
23.8
|
1.0
|
MN
|
B:MN238
|
4.7
|
45.3
|
1.0
|
N
|
B:TYR125
|
4.7
|
17.8
|
1.0
|
CD1
|
B:TYR125
|
4.8
|
26.6
|
1.0
|
N
|
B:SER126
|
4.8
|
28.6
|
1.0
|
CA
|
B:ASN127
|
4.9
|
27.6
|
1.0
|
C
|
B:SER126
|
4.9
|
31.4
|
1.0
|
CA
|
B:SER126
|
4.9
|
31.6
|
1.0
|
|
Calcium binding site 3 out
of 4 in 1v6j
Go back to
Calcium Binding Sites List in 1v6j
Calcium binding site 3 out
of 4 in the Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Ca237
b:29.3
occ:1.00
|
O
|
C:TYR125
|
2.7
|
21.4
|
1.0
|
OD1
|
C:ASP123
|
2.7
|
38.5
|
1.0
|
OD2
|
C:ASP132
|
2.7
|
39.1
|
1.0
|
OD1
|
C:ASN127
|
2.8
|
39.0
|
1.0
|
OD2
|
C:ASP123
|
2.8
|
40.2
|
1.0
|
CG
|
C:ASP123
|
3.1
|
36.0
|
1.0
|
CG
|
C:ASN127
|
3.5
|
38.3
|
1.0
|
C
|
C:TYR125
|
3.8
|
22.1
|
1.0
|
CB
|
C:ASN127
|
3.8
|
38.4
|
1.0
|
CG
|
C:ASP132
|
3.8
|
43.2
|
1.0
|
N
|
C:ASN127
|
3.9
|
41.2
|
1.0
|
CA
|
C:GLY104
|
4.3
|
43.9
|
1.0
|
OD1
|
C:ASP132
|
4.4
|
39.6
|
1.0
|
O
|
C:GLY104
|
4.5
|
47.0
|
1.0
|
CA
|
C:ASN127
|
4.5
|
37.5
|
1.0
|
CB
|
C:TYR125
|
4.5
|
48.9
|
1.0
|
ND2
|
C:ASN127
|
4.6
|
41.9
|
1.0
|
CA
|
C:TYR125
|
4.6
|
19.2
|
1.0
|
CB
|
C:ASP123
|
4.6
|
33.0
|
1.0
|
CD1
|
C:TYR125
|
4.6
|
54.4
|
1.0
|
O
|
C:ASP83
|
4.6
|
62.2
|
1.0
|
N
|
C:TYR125
|
4.7
|
18.3
|
1.0
|
CB
|
C:ASP83
|
4.7
|
44.4
|
1.0
|
N
|
C:SER126
|
4.7
|
38.9
|
1.0
|
OD1
|
C:ASP83
|
4.7
|
48.4
|
1.0
|
C
|
C:SER126
|
4.8
|
45.3
|
1.0
|
MN
|
C:MN238
|
4.8
|
48.3
|
1.0
|
CA
|
C:SER126
|
4.8
|
41.3
|
1.0
|
C
|
C:GLY104
|
4.9
|
45.2
|
1.0
|
CB
|
C:ASP132
|
5.0
|
40.6
|
1.0
|
|
Calcium binding site 4 out
of 4 in 1v6j
Go back to
Calcium Binding Sites List in 1v6j
Calcium binding site 4 out
of 4 in the Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of Peanut Lectin-Lactose Complex Crystallized in Orthorhombic Form at Acidic pH within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Ca237
b:28.2
occ:1.00
|
OD2
|
D:ASP123
|
2.8
|
24.4
|
1.0
|
O
|
D:TYR125
|
2.8
|
18.3
|
1.0
|
OD2
|
D:ASP132
|
2.8
|
37.6
|
1.0
|
OD1
|
D:ASP123
|
2.8
|
20.5
|
1.0
|
OD1
|
D:ASN127
|
3.0
|
30.5
|
1.0
|
CG
|
D:ASP123
|
3.1
|
22.8
|
1.0
|
CG
|
D:ASN127
|
3.3
|
28.5
|
1.0
|
CB
|
D:ASN127
|
3.7
|
30.2
|
1.0
|
CG
|
D:ASP132
|
3.9
|
36.2
|
1.0
|
C
|
D:TYR125
|
3.9
|
20.5
|
1.0
|
ND2
|
D:ASN127
|
4.0
|
28.3
|
1.0
|
CA
|
D:GLY104
|
4.0
|
26.4
|
1.0
|
N
|
D:ASN127
|
4.1
|
32.1
|
1.0
|
O
|
D:GLY104
|
4.3
|
26.7
|
1.0
|
OD1
|
D:ASP132
|
4.3
|
33.0
|
1.0
|
CB
|
D:ASP83
|
4.4
|
41.2
|
1.0
|
CA
|
D:ASN127
|
4.6
|
31.7
|
1.0
|
OD1
|
D:ASP83
|
4.6
|
45.4
|
1.0
|
O
|
D:ASP83
|
4.6
|
71.8
|
1.0
|
CB
|
D:ASP123
|
4.6
|
22.5
|
1.0
|
C
|
D:GLY104
|
4.7
|
25.9
|
1.0
|
CA
|
D:TYR125
|
4.7
|
18.6
|
1.0
|
CB
|
D:TYR125
|
4.7
|
34.5
|
1.0
|
C
|
D:SER126
|
4.8
|
41.6
|
1.0
|
N
|
D:TYR125
|
4.8
|
17.1
|
1.0
|
N
|
D:SER126
|
4.8
|
35.1
|
1.0
|
CD1
|
D:TYR125
|
4.8
|
36.5
|
1.0
|
CA
|
D:SER126
|
4.9
|
38.4
|
1.0
|
MN
|
D:MN238
|
5.0
|
45.8
|
1.0
|
|
Reference:
S.Kundhavai Natchiar,
A.Arockia Jeyaprakash,
T.N.Ramya,
C.J.Thomas,
K.Suguna,
A.Surolia,
M.Vijayan.
Structural Plasticity of Peanut Lectin: An X-Ray Analysis Involving Variation in pH, Ligand Binding and Crystal Structure. Acta Crystallogr.,Sect.D V. 60 211 2004.
ISSN: ISSN 0907-4449
PubMed: 14747696
DOI: 10.1107/S090744490302849X
Page generated: Thu Jul 11 23:55:51 2024
|