Calcium in PDB 1ven: Crystal Structure Analysis of Y164E/Maltose of Bacilus Cereus Beta-Amylase at pH 4.6

All present enzymatic activity of Crystal Structure Analysis of Y164E/Maltose of Bacilus Cereus Beta-Amylase at pH 4.6:
3.2.1.2;

The structure of Crystal Structure Analysis of Y164E/Maltose of Bacilus Cereus Beta-Amylase at pH 4.6, PDB code: 1ven was solved by A.Hirata, M.Adachi, S.Utsumi, B.Mikami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.93 / 2.02
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	57.489, 92.899, 65.962, 90.00, 102.36, 90.00
R / Rfree (%)	19.3 / 22.9

The binding sites of Calcium atom in the Crystal Structure Analysis of Y164E/Maltose of Bacilus Cereus Beta-Amylase at pH 4.6 (pdb code 1ven). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure Analysis of Y164E/Maltose of Bacilus Cereus Beta-Amylase at pH 4.6, PDB code: 1ven:

Calcium binding site 1 out of 1 in the Crystal Structure Analysis of Y164E/Maltose of Bacilus Cereus Beta-Amylase at pH 4.6


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure Analysis of Y164E/Maltose of Bacilus Cereus Beta-Amylase at pH 4.6 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca930 b:21.0 occ:1.00 OD1 A:ASP60 2.2 30.8 1.0 OE1 A:GLU144 2.4 28.6 1.0 OE1 A:GLN61 2.4 26.1 1.0 OE1 A:GLU141 2.4 25.3 1.0 OE2 A:GLU56 2.5 24.8 1.0 O A:HOH558 2.5 22.8 1.0 CG A:ASP60 3.4 30.6 1.0 CD A:GLU144 3.5 25.4 1.0 CD A:GLU56 3.5 26.8 1.0 CD A:GLN61 3.5 25.6 1.0 CD A:GLU141 3.5 26.9 1.0 OE1 A:GLU56 3.8 25.9 1.0 N A:GLN61 3.8 28.3 1.0 CB A:GLU144 3.9 20.7 1.0 NZ A:LYS140 3.9 22.2 1.0 OE2 A:GLU141 4.1 29.6 1.0 CH2 A:TRP106 4.1 28.7 1.0 OD2 A:ASP60 4.2 33.3 1.0 CG A:GLN61 4.3 26.6 1.0 OE2 A:GLU144 4.3 28.4 1.0 CG A:GLU144 4.3 23.9 1.0 CA A:ASP60 4.4 31.1 1.0 CA A:GLU141 4.4 18.7 1.0 CB A:ASP60 4.4 30.8 1.0 C A:ASP60 4.5 30.3 1.0 NE2 A:GLN61 4.5 23.4 1.0 CA A:GLN61 4.6 29.6 1.0 CZ A:PHE63 4.7 29.1 1.0 O A:GLU141 4.7 17.9 1.0 CG A:GLU141 4.7 22.6 1.0 CB A:GLU141 4.8 19.4 1.0 CG A:GLU56 4.8 26.9 1.0 CZ2 A:TRP106 4.9 28.0 1.0 CZ3 A:TRP106 5.0 28.1 1.0

A.Hirata, M.Adachi, S.Utsumi, B.Mikami. Engineering of the pH Optimum of Bacillus Cereus Beta-Amylase: Conversion of the pH Optimum From A Bacterial Type to A Higher-Plant Type Biochemistry V. 43 12523 2004.
ISSN: ISSN 0006-2960
PubMed: 15449941
DOI: 10.1021/BI049173H