Calcium in PDB 1wc0: Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp

All present enzymatic activity of Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp:
4.6.1.1;

The structure of Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp, PDB code: 1wc0 was solved by C.Steegborn, T.N.Litvin, L.R.Levin, J.Buck, H.Wu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.88 / 2.4
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	53.672, 71.539, 99.572, 90.00, 90.00, 90.00
R / Rfree (%)	21.9 / 27.1

The binding sites of Calcium atom in the Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp (pdb code 1wc0). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp, PDB code: 1wc0:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca2100 b:22.5 occ:1.00 O1B A:APC1500 2.1 37.2 1.0 O A:HOH2070 2.3 22.9 1.0 OD1 A:ASP1017 2.4 34.8 1.0 OD1 A:ASP1061 2.5 28.4 1.0 O A:ILE1018 2.5 31.7 1.0 OD2 A:ASP1017 2.9 33.4 1.0 O3G A:APC1500 2.9 44.1 1.0 CG A:ASP1017 2.9 33.6 1.0 CG A:ASP1061 3.3 25.0 1.0 OD2 A:ASP1061 3.3 30.1 1.0 PB A:APC1500 3.4 36.8 1.0 C A:ILE1018 3.7 34.0 1.0 PG A:APC1500 3.8 41.8 1.0 O1A A:APC1500 3.9 29.4 1.0 O3B A:APC1500 4.0 39.5 1.0 N A:ILE1018 4.1 32.8 1.0 C3A A:APC1500 4.2 34.1 1.0 O1G A:APC1500 4.3 41.9 1.0 CB A:ASP1017 4.4 31.4 1.0 CA A:ILE1018 4.6 32.5 1.0 O2B A:APC1500 4.6 38.5 1.0 CG2 A:VAL1019 4.6 38.1 1.0 CB A:ASP1061 4.6 22.6 1.0 N A:VAL1019 4.7 37.3 1.0 PA A:APC1500 4.7 35.0 1.0 O A:HOH2068 4.7 30.1 1.0 CA A:VAL1019 4.7 39.5 1.0 C A:ASP1017 4.8 32.1 1.0 CG1 A:ILE1018 5.0 28.5 1.0 CA A:ASP1017 5.0 30.9 1.0

Calcium binding site 2 out of 2 in the Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca2100 b:21.7 occ:1.00 O1B B:APC1500 2.0 45.7 1.0 O B:HOH2070 2.3 10.3 1.0 OD1 B:ASP1017 2.3 29.6 1.0 O B:ILE1018 2.4 30.1 1.0 OD2 B:ASP1061 2.6 24.8 1.0 O3G B:APC1500 2.6 49.9 1.0 OD2 B:ASP1017 2.8 29.0 1.0 CG B:ASP1017 2.9 28.6 1.0 CG B:ASP1061 3.4 25.1 1.0 PB B:APC1500 3.4 43.1 1.0 OD1 B:ASP1061 3.5 31.2 1.0 C B:ILE1018 3.5 31.7 1.0 PG B:APC1500 3.7 47.6 1.0 O3B B:APC1500 3.9 45.4 1.0 N B:ILE1018 3.9 25.9 1.0 O1A B:APC1500 4.1 37.6 1.0 O1G B:APC1500 4.2 49.2 1.0 O B:HOH2018 4.3 17.0 1.0 CA B:ILE1018 4.3 29.9 1.0 C3A B:APC1500 4.4 40.0 1.0 CB B:ASP1017 4.4 24.8 1.0 O2B B:APC1500 4.4 44.5 1.0 N B:VAL1019 4.4 35.2 1.0 CA B:VAL1019 4.6 39.0 1.0 CG2 B:VAL1019 4.6 40.1 1.0 CG1 B:ILE1018 4.7 30.2 1.0 C B:ASP1017 4.7 23.6 1.0 CB B:ASP1061 4.7 22.7 1.0 O B:HOH2015 4.8 18.2 1.0 O B:ASP1061 4.8 19.2 1.0 O B:HOH2068 4.9 42.7 1.0 CA B:ASP1017 4.9 22.0 1.0 PA B:APC1500 4.9 40.8 1.0 C B:ASP1061 4.9 16.4 1.0 O2G B:APC1500 5.0 47.8 1.0

C.Steegborn, T.N.Litvin, L.R.Levin, J.Buck, H.Wu. Bicarbonate Activation of Adenylyl Cyclase Via Promotion of Catalytic Active Site Closure and Metal Recruitment Nat.Struct.Mol.Biol. V. 12 32 2005.
ISSN: ISSN 1545-9993
PubMed: 15619637
DOI: 10.1038/NSMB880