Calcium in PDB 1wza: Crystal Structure of Alpha-Amylase From H.Orenii

All present enzymatic activity of Crystal Structure of Alpha-Amylase From H.Orenii:
3.2.1.1;

The structure of Crystal Structure of Alpha-Amylase From H.Orenii, PDB code: 1wza was solved by N.Sivakumar, K.Swaminathan, N.Li, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 1.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	55.126, 61.658, 147.625, 90.00, 90.00, 90.00
R / Rfree (%)	18.6 / 20.9

The binding sites of Calcium atom in the Crystal Structure of Alpha-Amylase From H.Orenii (pdb code 1wza). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Alpha-Amylase From H.Orenii, PDB code: 1wza:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Alpha-Amylase From H.Orenii


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Alpha-Amylase From H.Orenii within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca601 b:8.1 occ:1.00 O A:ILE50 2.5 8.7 1.0 OD1 A:ASP44 2.5 9.6 1.0 OD2 A:ASP52 2.5 10.4 1.0 OD1 A:ASP46 2.5 11.7 1.0 OD1 A:ASP48 2.5 10.5 1.0 O A:HOH629 2.6 13.2 1.0 CG A:ASP48 3.3 10.6 1.0 CG A:ASP44 3.4 10.8 1.0 CG A:ASP46 3.5 16.9 1.0 OD2 A:ASP48 3.5 12.2 1.0 C A:ILE50 3.6 8.8 1.0 CG A:ASP52 3.6 8.7 1.0 OD2 A:ASP46 4.0 19.5 1.0 O A:HOH929 4.0 36.3 1.0 CB A:ASP52 4.0 8.3 1.0 N A:ILE50 4.1 9.7 1.0 CB A:ASP44 4.1 8.7 1.0 N A:ASP46 4.1 10.9 1.0 CB A:ILE50 4.1 11.6 1.0 CA A:ILE50 4.2 9.3 1.0 CA A:ASP44 4.2 8.1 1.0 OD2 A:ASP44 4.3 10.9 1.0 N A:SER45 4.3 9.8 1.0 N A:ASP48 4.4 12.3 1.0 CB A:ASP48 4.5 12.0 1.0 C A:ASP44 4.5 9.9 1.0 C A:GLY51 4.6 6.5 1.0 N A:ASP52 4.6 7.1 1.0 OD1 A:ASP52 4.6 10.3 1.0 N A:GLY51 4.7 7.4 1.0 CB A:ASP46 4.7 12.4 1.0 CA A:ASP46 4.7 11.9 1.0 O A:ASP104 4.7 11.5 1.0 N A:GLY47 4.8 11.2 1.0 O A:GLY51 4.8 7.3 1.0 N A:GLY49 4.8 11.8 1.0 CA A:ASP48 4.9 12.6 1.0 CA A:GLY51 4.9 6.4 1.0 C A:ASP46 4.9 11.3 1.0 O A:HOH917 5.0 32.8 1.0 CG2 A:ILE50 5.0 9.9 1.0 CA A:ASP52 5.0 7.3 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Alpha-Amylase From H.Orenii


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Alpha-Amylase From H.Orenii within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca602 b:17.9 occ:1.00 O A:ASP67 2.5 14.8 1.0 OG1 A:THR70 2.6 13.9 1.0 OD1 A:ASP73 2.6 10.9 1.0 OD1 A:ASP65 2.6 9.9 1.0 O A:THR70 2.6 12.6 1.0 O A:HOH671 2.7 16.1 1.0 CG A:ASP73 3.2 11.0 1.0 OD2 A:ASP73 3.3 11.9 1.0 C A:THR70 3.3 12.7 1.0 CG A:ASP65 3.4 11.4 1.0 C A:ASP67 3.5 15.1 1.0 OD2 A:ASP65 3.6 10.1 1.0 CB A:THR70 3.7 12.2 1.0 N A:THR70 3.8 13.1 1.0 CA A:THR70 3.8 12.4 1.0 NE2 A:HIS426 3.8 14.3 1.0 N A:ASP67 4.2 14.2 1.0 N A:ILE71 4.3 12.7 1.0 CA A:ASP67 4.3 15.0 1.0 O A:HOH739 4.3 21.4 1.0 N A:PRO68 4.3 16.2 1.0 CA A:PRO68 4.4 16.4 1.0 CB A:ASP73 4.5 10.2 1.0 CB A:ASP67 4.5 16.5 1.0 CG2 A:THR70 4.5 12.8 1.0 C A:PRO68 4.6 16.8 1.0 CD2 A:HIS426 4.6 13.7 1.0 CA A:ILE71 4.6 14.0 1.0 N A:ALA72 4.7 14.2 1.0 N A:ASP65 4.7 10.2 1.0 N A:ASP73 4.8 10.8 1.0 CB A:ASP65 4.8 9.1 1.0 N A:GLU69 4.8 17.5 1.0 CE1 A:HIS426 4.8 12.6 1.0 C A:GLU69 5.0 17.3 1.0

N.Sivakumar, N.Li, J.W.Tang, B.K.Patel, K.Swaminathan. Crystal Structure of Amya Lacks Acidic Surface and Provide Insights Into Protein Stability at Poly-Extreme Condition. Febs Lett. V. 580 2646 2006.
ISSN: ISSN 0014-5793
PubMed: 16647060
DOI: 10.1016/J.FEBSLET.2006.04.017