Calcium in PDB 1xgz: Structure of the N298S Variant of Human Pancreatic Alpha-Amylase

All present enzymatic activity of Structure of the N298S Variant of Human Pancreatic Alpha-Amylase:
3.2.1.1;

The structure of Structure of the N298S Variant of Human Pancreatic Alpha-Amylase, PDB code: 1xgz was solved by R.Maurus, A.Begum, H.H.Kuo, A.Racaza, S.Numao, C.M.Overall, S.G.Withers, G.D.Brayer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	52.910, 68.900, 131.770, 90.00, 90.00, 90.00
R / Rfree (%)	15.5 / 20

The binding sites of Calcium atom in the Structure of the N298S Variant of Human Pancreatic Alpha-Amylase (pdb code 1xgz). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Structure of the N298S Variant of Human Pancreatic Alpha-Amylase, PDB code: 1xgz:

Calcium binding site 1 out of 1 in the Structure of the N298S Variant of Human Pancreatic Alpha-Amylase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of the N298S Variant of Human Pancreatic Alpha-Amylase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca498 b:18.3 occ:1.00 O A:ARG158 2.5 14.3 1.0 O A:HIS201 2.6 12.7 1.0 OD1 A:ASN100 2.6 12.6 1.0 O A:HOH506 2.6 14.3 1.0 OD2 A:ASP167 2.7 14.5 1.0 OD1 A:ASP167 2.7 11.9 1.0 O A:HOH501 2.8 20.7 1.0 O A:HOH531 2.8 16.8 1.0 CG A:ASP167 3.0 12.9 1.0 C A:ARG158 3.6 15.0 1.0 CG A:ASN100 3.6 13.8 1.0 C A:HIS201 3.7 14.1 1.0 ND2 A:ASN100 4.1 13.3 1.0 CA A:ARG158 4.2 14.6 1.0 CB A:HIS201 4.3 12.9 1.0 O A:ASN100 4.4 16.1 1.0 CB A:ASP167 4.5 13.4 1.0 ND2 A:ASN137 4.5 18.5 1.0 O A:HOH546 4.5 16.9 1.0 O A:CYS160 4.5 16.9 1.0 CA A:HIS201 4.6 13.4 1.0 N A:ASP159 4.6 14.1 1.0 N A:MET202 4.6 13.8 1.0 CA A:MET202 4.7 14.1 1.0 O A:VAL157 4.7 16.4 1.0 CG A:MET202 4.7 15.3 1.0 CA A:ASP159 4.8 15.6 1.0 O A:LEU168 4.9 15.6 1.0 O A:HOH515 4.9 14.4 1.0 CB A:ASN100 4.9 12.9 1.0

R.Maurus, A.Begum, H.H.Kuo, A.Racaza, S.Numao, C.Andersen, J.W.Tams, J.Vind, C.M.Overall, S.G.Withers, G.D.Brayer. Structural and Mechanistic Studies of Chloride Induced Activation of Human Pancreatic Alpha-Amylase Protein Sci. V. 14 743 2005.
ISSN: ISSN 0961-8368
PubMed: 15722449
DOI: 10.1110/PS.041079305