Calcium in PDB 1xv8: Crystal Structure of Human Salivary Alpha-Amylase Dimer

All present enzymatic activity of Crystal Structure of Human Salivary Alpha-Amylase Dimer:
3.2.1.1;

The structure of Crystal Structure of Human Salivary Alpha-Amylase Dimer, PDB code: 1xv8 was solved by S.Z.Fisher, L.Govindasamy, C.K.Tu, D.N.Silverman, H.Rajaniemi, R.Mckenna, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 3.00
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	150.812, 72.270, 91.106, 90.00, 102.80, 90.00
R / Rfree (%)	22.8 / 27.1

The structure of Crystal Structure of Human Salivary Alpha-Amylase Dimer also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Calcium atom in the Crystal Structure of Human Salivary Alpha-Amylase Dimer (pdb code 1xv8). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Human Salivary Alpha-Amylase Dimer, PDB code: 1xv8:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Human Salivary Alpha-Amylase Dimer


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Human Salivary Alpha-Amylase Dimer within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca497 b:12.1 occ:1.00 OD1 A:ASP167 2.1 13.2 1.0 O A:HIS201 2.2 18.5 1.0 OD1 A:ASN100 2.3 17.1 1.0 O A:ARG158 2.4 23.4 1.0 O A:HOH510 2.5 20.5 1.0 OD2 A:ASP167 2.7 12.4 1.0 CG A:ASP167 2.7 10.3 1.0 C A:ARG158 3.2 21.8 1.0 CG A:ASN100 3.2 17.8 1.0 C A:HIS201 3.3 17.1 1.0 ND2 A:ASN100 3.5 15.9 1.0 CA A:ARG158 3.5 20.6 1.0 CB A:HIS201 3.7 16.4 1.0 CA A:HIS201 4.0 17.4 1.0 O A:VAL157 4.0 21.3 1.0 CB A:ASP167 4.2 9.8 1.0 N A:ASP159 4.3 21.2 1.0 O A:CYS160 4.3 22.0 1.0 CB A:ARG158 4.4 21.2 1.0 N A:MET202 4.4 16.4 1.0 CB A:ASN100 4.6 17.4 1.0 ND2 A:ASN137 4.6 23.7 1.0 N A:ARG158 4.6 18.6 1.0 O A:ASN100 4.7 16.1 1.0 CA A:MET202 4.7 15.8 1.0 C A:VAL157 4.8 18.9 1.0 CA A:ASP159 4.8 21.2 1.0 CG A:MET202 4.8 14.6 1.0 N A:CYS160 4.9 19.4 1.0 CG A:HIS201 5.0 16.2 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Human Salivary Alpha-Amylase Dimer


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Human Salivary Alpha-Amylase Dimer within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca497 b:12.1 occ:1.00 OD1 B:ASP167 2.1 13.2 1.0 O B:HIS201 2.2 18.5 1.0 OD1 B:ASN100 2.3 17.1 1.0 O B:ARG158 2.4 23.4 1.0 O B:HOH513 2.5 20.5 1.0 OD2 B:ASP167 2.7 12.4 1.0 CG B:ASP167 2.7 10.3 1.0 C B:ARG158 3.2 21.8 1.0 CG B:ASN100 3.2 17.8 1.0 C B:HIS201 3.3 17.1 1.0 ND2 B:ASN100 3.5 15.9 1.0 CA B:ARG158 3.5 20.6 1.0 CB B:HIS201 3.7 16.4 1.0 CA B:HIS201 4.0 17.4 1.0 O B:VAL157 4.0 21.3 1.0 CB B:ASP167 4.2 9.8 1.0 N B:ASP159 4.3 21.2 1.0 O B:CYS160 4.3 22.0 1.0 CB B:ARG158 4.4 21.2 1.0 N B:MET202 4.4 16.4 1.0 CB B:ASN100 4.6 17.4 1.0 ND2 B:ASN137 4.6 23.7 1.0 N B:ARG158 4.6 18.6 1.0 O B:ASN100 4.7 16.1 1.0 CA B:MET202 4.7 15.8 1.0 C B:VAL157 4.8 18.9 1.0 CA B:ASP159 4.8 21.2 1.0 CG B:MET202 4.8 14.6 1.0 N B:CYS160 4.9 19.4 1.0 CG B:HIS201 5.0 16.2 1.0

S.Z.Fisher, L.Govindasamy, C.K.Tu, D.N.Silverman, H.Rajaniemi, R.Mckenna. Crystal Structure of Human Salivary Alpha-Amylase Dimer To Be Published.