Calcium in PDB 1yfq: High Resolution S. Cerevisiae BUB3 Mitotic Checkpoint Protein

The structure of High Resolution S. Cerevisiae BUB3 Mitotic Checkpoint Protein, PDB code: 1yfq was solved by D.K.Wilson, D.Cerna, E.Chew, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	52.274, 74.089, 94.090, 90.00, 90.00, 90.00
R / Rfree (%)	15.2 / 18.6

The binding sites of Calcium atom in the High Resolution S. Cerevisiae BUB3 Mitotic Checkpoint Protein (pdb code 1yfq). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the High Resolution S. Cerevisiae BUB3 Mitotic Checkpoint Protein, PDB code: 1yfq:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the High Resolution S. Cerevisiae BUB3 Mitotic Checkpoint Protein


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of High Resolution S. Cerevisiae BUB3 Mitotic Checkpoint Protein within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca800 b:11.1 occ:1.00 O A:HOH2108 2.4 12.4 1.0 O A:HOH2090 2.4 10.8 1.0 OE1 A:GLU258 2.5 8.5 1.0 O A:HOH2155 2.5 15.1 1.0 O A:HOH2331 2.5 16.5 1.0 O A:HOH2191 2.5 13.2 1.0 O A:HOH2330 2.5 15.6 1.0 OE2 A:GLU258 2.6 11.2 1.0 CD A:GLU258 2.9 8.2 1.0 OD2 A:ASP157 4.2 9.0 1.0 O A:HOH2192 4.3 18.5 1.0 NH1 A:ARG106 4.4 23.8 1.0 CG A:GLU258 4.4 8.3 1.0 O A:HOH2089 4.5 8.3 1.0 O A:ILE257 4.5 6.9 1.0 O A:HOH2107 4.5 12.7 1.0 CG A:ASP157 4.5 7.1 1.0 O A:HOH2235 4.6 12.3 1.0 O A:VAL199 4.6 6.9 1.0 O A:HOH2132 4.6 17.4 1.0 OD1 A:ASP157 4.7 8.0 1.0 O A:HOH2236 4.9 18.9 1.0

Calcium binding site 2 out of 4 in the High Resolution S. Cerevisiae BUB3 Mitotic Checkpoint Protein


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of High Resolution S. Cerevisiae BUB3 Mitotic Checkpoint Protein within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca801 b:14.9 occ:1.00 OG1 A:THR315 2.3 12.3 1.0 OD2 A:ASP313 2.3 9.7 1.0 O A:HOH2128 2.3 19.0 1.0 O A:HOH2436 2.3 20.3 1.0 O A:HOH2437 2.4 22.5 1.0 O A:HOH2382 2.4 17.9 1.0 CG A:ASP313 3.1 7.9 1.0 OD1 A:ASP313 3.1 10.2 1.0 CB A:THR315 3.3 9.6 1.0 N A:THR315 4.1 8.2 1.0 CG2 A:THR315 4.3 11.6 1.0 CA A:THR315 4.3 8.4 1.0 CB A:ASP313 4.5 8.3 1.0 O A:HOH2433 4.6 27.9 1.0 O A:HOH2401 4.7 27.7 1.0 O A:HOH2383 4.8 19.8 1.0 OD2 A:ASP295 4.8 15.3 1.0 O A:HOH2131 4.9 14.2 1.0 ND2 A:ASN329 4.9 15.2 1.0 O A:HOH2515 5.0 39.3 1.0 CA A:ASP313 5.0 7.3 1.0

Calcium binding site 3 out of 4 in the High Resolution S. Cerevisiae BUB3 Mitotic Checkpoint Protein


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of High Resolution S. Cerevisiae BUB3 Mitotic Checkpoint Protein within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca802 b:36.1 occ:1.00 O A:HOH2378 2.2 21.8 1.0 O A:HOH2282 2.3 21.4 1.0 OD1 A:ASP247 2.3 13.2 1.0 O A:HOH2379 2.4 27.6 1.0 O A:HOH2384 2.4 25.7 1.0 O A:HOH2215 2.4 28.6 1.0 O A:HOH2465 3.4 32.9 1.0 CG A:ASP247 3.5 11.8 1.0 O A:HOH2281 3.8 21.8 1.0 NZ A:LYS291 3.9 25.9 1.0 OD2 A:ASP247 4.0 11.3 1.0 O A:HOH2213 4.0 15.3 1.0 O A:HOH2113 4.3 23.7 1.0 OE1 A:GLU294 4.4 45.8 1.0 O A:HOH2480 4.4 32.1 1.0 O A:ASP272 4.6 8.3 1.0 CB A:ASP247 4.7 11.2 1.0 O A:HOH2293 4.7 28.2 1.0 O A:HOH2559 4.7 45.6 1.0 O A:LYS246 4.8 19.7 1.0 O A:HOH2365 4.8 16.1 1.0 O A:HOH2130 4.9 12.1 1.0 CA A:ASP247 4.9 11.5 1.0 O A:GLU294 5.0 12.3 1.0

Calcium binding site 4 out of 4 in the High Resolution S. Cerevisiae BUB3 Mitotic Checkpoint Protein


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of High Resolution S. Cerevisiae BUB3 Mitotic Checkpoint Protein within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca803 b:18.6 occ:1.00 O A:HOH2354 2.2 27.4 1.0 OD1 A:ASN184 2.2 20.9 1.0 O A:HOH2355 2.2 27.6 1.0 O A:HOH2372 2.4 25.4 1.0 O A:GLY185 2.5 19.3 1.0 O A:HOH2334 2.6 40.9 1.0 CG A:ASN184 3.4 22.3 1.0 C A:GLY185 3.7 16.2 1.0 ND2 A:ASN184 3.8 34.8 1.0 O A:HOH2335 4.1 21.5 1.0 OG1 A:THR186 4.2 16.1 1.0 N A:GLY185 4.4 14.5 1.0 O A:HOH2302 4.5 17.4 1.0 N A:THR186 4.5 13.1 1.0 CA A:THR186 4.6 12.9 1.0 C A:ASN184 4.6 17.7 1.0 O A:HOH2402 4.6 27.5 1.0 O A:HOH2201 4.6 23.2 1.0 CA A:GLY185 4.6 15.9 1.0 CB A:ASN184 4.7 19.3 1.0 N A:ASN184 4.9 16.0 1.0 O A:ASN184 4.9 21.2 1.0 CB A:THR186 5.0 13.9 1.0 CA A:ASN184 5.0 15.7 1.0

D.K.Wilson, D.Cerna, E.Chew. The 1.1-A Structure of the Spindle Checkpoint Protein BUB3P Reveals Functional Regions. J.Biol.Chem. V. 280 13944 2005.
ISSN: ISSN 0021-9258
PubMed: 15644329
DOI: 10.1074/JBC.M412919200