Calcium in PDB 2a3z: Ternary Complex of the WH2 Domain of Wasp with Actin-Dnase I

All present enzymatic activity of Ternary Complex of the WH2 Domain of Wasp with Actin-Dnase I:
3.1.21.1;

The structure of Ternary Complex of the WH2 Domain of Wasp with Actin-Dnase I, PDB code: 2a3z was solved by D.Chereau, F.Kerff, R.Dominguez, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.10 / 2.08
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	153.270, 41.798, 119.418, 90.00, 108.68, 90.00
R / Rfree (%)	15.3 / 21

The structure of Ternary Complex of the WH2 Domain of Wasp with Actin-Dnase I also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Calcium atom in the Ternary Complex of the WH2 Domain of Wasp with Actin-Dnase I (pdb code 2a3z). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Ternary Complex of the WH2 Domain of Wasp with Actin-Dnase I, PDB code: 2a3z:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Ternary Complex of the WH2 Domain of Wasp with Actin-Dnase I


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ternary Complex of the WH2 Domain of Wasp with Actin-Dnase I within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1381 b:24.7 occ:1.00 O A:HOH1386 2.3 24.0 1.0 O A:HOH1420 2.4 25.5 1.0 O1B A:ATP1380 2.4 25.5 1.0 O A:HOH1391 2.4 23.3 1.0 O2G A:ATP1380 2.5 24.4 1.0 O A:HOH1394 2.5 30.6 1.0 O A:HOH1393 2.6 29.7 1.0 PG A:ATP1380 3.6 23.4 1.0 PB A:ATP1380 3.7 23.3 1.0 O3G A:ATP1380 3.9 25.7 1.0 O3B A:ATP1380 4.0 22.3 1.0 O A:HOH1390 4.2 23.5 1.0 NE2 A:GLN137 4.2 25.6 1.0 O3A A:ATP1380 4.3 23.1 1.0 CA A:GLY13 4.4 27.8 1.0 O1A A:ATP1380 4.4 26.7 1.0 CD A:GLN137 4.5 31.9 1.0 OD2 A:ASP154 4.5 34.5 1.0 NZ A:LYS18 4.5 27.4 1.0 O A:HOH1469 4.6 33.5 1.0 O A:HOH1439 4.6 34.2 1.0 OD1 A:ASP11 4.6 33.7 1.0 OD1 A:ASP154 4.8 38.3 1.0 OD2 A:ASP11 4.8 31.7 1.0 OE1 A:GLN137 4.8 35.1 1.0 PA A:ATP1380 4.9 23.8 1.0 CG A:GLN137 4.9 26.3 1.0 O1G A:ATP1380 4.9 24.4 1.0 O2B A:ATP1380 4.9 23.2 1.0

Calcium binding site 2 out of 2 in the Ternary Complex of the WH2 Domain of Wasp with Actin-Dnase I


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Ternary Complex of the WH2 Domain of Wasp with Actin-Dnase I within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1273 b:26.2 occ:1.00 O B:THR205 2.4 28.6 1.0 O B:HOH1397 2.4 31.7 1.0 O B:THR207 2.4 27.8 1.0 O B:HOH1391 2.5 24.3 1.0 OD2 B:ASP201 2.5 27.8 1.0 O B:THR203 2.5 26.3 1.0 OG1 B:THR203 2.6 25.0 1.0 OD1 B:ASP201 2.6 31.0 1.0 CG B:ASP201 3.0 26.9 1.0 C B:THR203 3.4 26.8 1.0 C B:THR205 3.6 28.2 1.0 C B:THR207 3.7 27.6 1.0 CB B:THR203 3.8 25.5 1.0 CA B:THR203 4.0 25.4 1.0 N B:THR203 4.1 25.4 1.0 N B:THR207 4.2 29.1 1.0 N B:THR205 4.3 27.4 1.0 C B:SER206 4.4 29.5 1.0 N B:ALA204 4.4 27.2 1.0 C B:ALA204 4.4 28.4 1.0 CB B:ASP201 4.5 27.1 1.0 N B:SER206 4.5 28.6 1.0 CA B:SER206 4.5 29.2 1.0 CA B:THR205 4.5 27.8 1.0 CA B:THR207 4.5 28.3 1.0 N B:ASN208 4.6 27.5 1.0 CA B:ASN208 4.6 27.4 1.0 OG1 B:THR207 4.7 30.8 1.0 OG1 B:THR205 4.7 31.0 1.0 CA B:ALA204 4.7 28.8 1.0 OH B:TYR253 4.8 28.5 1.0 O B:ALA204 4.8 28.4 1.0 CE2 B:TYR253 4.9 28.4 1.0 O B:SER206 5.0 31.1 1.0

D.Chereau, F.Kerff, P.Graceffa, Z.Grabarek, K.Langsetmo, R.Dominguez. Actin-Bound Structures of Wiskott-Aldrich Syndrome Protein (Wasp)-Homology Domain 2 and the Implications For Filament Assembly Proc.Natl.Acad.Sci.Usa V. 102 16644 2005.
ISSN: ISSN 0027-8424
PubMed: 16275905
DOI: 10.1073/PNAS.0507021102