Calcium in PDB 2aaa: Calcium Binding in Alpha-Amylases: An X-Ray Diffraction Study at 2.1 Angstroms Resolution of Two Enzymes From Aspergillus

All present enzymatic activity of Calcium Binding in Alpha-Amylases: An X-Ray Diffraction Study at 2.1 Angstroms Resolution of Two Enzymes From Aspergillus:
3.2.1.1;

The structure of Calcium Binding in Alpha-Amylases: An X-Ray Diffraction Study at 2.1 Angstroms Resolution of Two Enzymes From Aspergillus, PDB code: 2aaa was solved by L.Brady, A.M.Brzozowski, Z.Derewenda, E.J.Dodson, G.G.Dodson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	7.00 / 2.12
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	81.100, 98.300, 138.000, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Calcium atom in the Calcium Binding in Alpha-Amylases: An X-Ray Diffraction Study at 2.1 Angstroms Resolution of Two Enzymes From Aspergillus (pdb code 2aaa). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Calcium Binding in Alpha-Amylases: An X-Ray Diffraction Study at 2.1 Angstroms Resolution of Two Enzymes From Aspergillus, PDB code: 2aaa:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Calcium Binding in Alpha-Amylases: An X-Ray Diffraction Study at 2.1 Angstroms Resolution of Two Enzymes From Aspergillus


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Calcium Binding in Alpha-Amylases: An X-Ray Diffraction Study at 2.1 Angstroms Resolution of Two Enzymes From Aspergillus within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca485 b:17.2 occ:1.00 O A:GLU210 2.4 10.2 1.0 O A:HOH495 2.5 17.3 1.0 OD1 A:ASP121 2.6 11.9 1.0 O A:HOH590 2.6 12.9 1.0 O A:HOH499 2.6 11.2 1.0 OD2 A:ASP175 2.6 17.4 1.0 O A:GLU162 2.6 10.6 1.0 OD1 A:ASP175 2.8 16.3 1.0 CG A:ASP175 3.0 18.4 1.0 C A:GLU210 3.6 12.1 1.0 C A:GLU162 3.6 10.3 1.0 CG A:ASP121 3.7 10.9 1.0 CB A:GLU210 4.0 10.6 1.0 OD2 A:ASP121 4.1 13.7 1.0 CA A:GLU162 4.2 10.4 1.0 CA A:GLU210 4.3 11.5 1.0 O A:LEU176 4.4 10.9 1.0 CE1 A:HIS147 4.5 13.3 1.0 ND1 A:HIS147 4.5 13.3 1.0 N A:VAL211 4.5 14.0 1.0 CG2 A:VAL211 4.6 11.7 1.0 CB A:ASP175 4.6 9.9 1.0 O A:ASP121 4.7 11.8 1.0 N A:ASP163 4.7 10.2 1.0 CA A:VAL211 4.7 13.5 1.0 O A:VAL161 4.7 11.5 1.0 O A:CYS164 4.8 12.9 1.0 CB A:ASP121 4.8 5.0 1.0 CA A:ASP163 4.9 13.1 1.0

Calcium binding site 2 out of 2 in the Calcium Binding in Alpha-Amylases: An X-Ray Diffraction Study at 2.1 Angstroms Resolution of Two Enzymes From Aspergillus


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Calcium Binding in Alpha-Amylases: An X-Ray Diffraction Study at 2.1 Angstroms Resolution of Two Enzymes From Aspergillus within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca486 b:60.3 occ:1.00 O A:HOH507 2.5 27.9 1.0 O A:HOH599 2.6 18.9 1.0 OD1 A:ASP206 2.6 12.4 1.0 OE1 A:GLU230 2.7 13.8 1.0 O A:HOH801 2.7 43.0 1.0 O A:HOH789 2.8 56.5 1.0 O A:HOH800 2.8 61.3 1.0 OE2 A:GLU230 2.9 15.0 1.0 CD A:GLU230 3.1 16.2 1.0 CG A:ASP206 3.5 12.2 1.0 OD2 A:ASP206 3.7 12.4 1.0 OD2 A:ASP297 4.1 16.6 1.0 O A:HOH713 4.2 30.9 1.0 OG A:SER207 4.3 11.0 1.0 CG A:GLU230 4.5 13.9 1.0 N A:SER207 4.5 11.8 1.0 NH2 A:ARG204 4.5 14.6 1.0 O A:HOH628 4.5 29.1 1.0 O A:HOH513 4.6 8.8 1.0 CB A:ASP206 4.8 11.6 1.0 NH1 A:ARG204 4.9 14.3 1.0

E.Boel, L.Brady, A.M.Brzozowski, Z.Derewenda, G.G.Dodson, V.J.Jensen, S.B.Petersen, H.Swift, L.Thim, H.F.Woldike. Calcium Binding in Alpha-Amylases: An X-Ray Diffraction Study at 2.1-A Resolution of Two Enzymes From Aspergillus. Biochemistry V. 29 6244 1990.
ISSN: ISSN 0006-2960
PubMed: 2207069
DOI: 10.1021/BI00478A019