Calcium in PDB 2aad: The Role of Histidine-40 in Ribonuclease T1 Catalysis: Three- Dimensional Structures of the Partially Active HIS40LYS Mutant

All present enzymatic activity of The Role of Histidine-40 in Ribonuclease T1 Catalysis: Three- Dimensional Structures of the Partially Active HIS40LYS Mutant:
3.1.27.3;

The structure of The Role of Histidine-40 in Ribonuclease T1 Catalysis: Three- Dimensional Structures of the Partially Active HIS40LYS Mutant, PDB code: 2aad was solved by I.Zegers, P.Verhelst, C.W.Choe, J.Steyaert, U.Heinemann, L.Wyns, W.Saenger, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	49.200, 48.190, 40.160, 90.00, 90.26, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Calcium atom in the The Role of Histidine-40 in Ribonuclease T1 Catalysis: Three- Dimensional Structures of the Partially Active HIS40LYS Mutant (pdb code 2aad). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the The Role of Histidine-40 in Ribonuclease T1 Catalysis: Three- Dimensional Structures of the Partially Active HIS40LYS Mutant, PDB code: 2aad:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the The Role of Histidine-40 in Ribonuclease T1 Catalysis: Three- Dimensional Structures of the Partially Active HIS40LYS Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of The Role of Histidine-40 in Ribonuclease T1 Catalysis: Three- Dimensional Structures of the Partially Active HIS40LYS Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca106 b:29.7 occ:1.00 OD2 A:ASP15 2.3 16.1 1.0 OD1 A:ASP15 2.4 15.1 1.0 CG A:ASP15 2.6 17.3 1.0 O A:HOH136 2.6 17.9 1.0 O A:HOH142 2.6 17.3 1.0 O A:HOH177 2.7 41.1 1.0 O A:HOH143 2.7 25.1 1.0 O A:HOH181 3.0 36.2 1.0 CB A:ASP15 4.1 17.6 1.0 O A:HOH124 4.3 17.6 1.0 OG A:SER12 4.5 15.2 1.0 O A:HOH110 4.6 15.8 1.0 O A:HOH173 4.6 36.6 1.0 O A:HOH111 4.8 26.5 1.0 O A:CYS10 4.8 13.0 1.0 N A:SER12 4.9 11.8 1.0 CA A:GLY65 4.9 16.9 1.0

Calcium binding site 2 out of 2 in the The Role of Histidine-40 in Ribonuclease T1 Catalysis: Three- Dimensional Structures of the Partially Active HIS40LYS Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of The Role of Histidine-40 in Ribonuclease T1 Catalysis: Three- Dimensional Structures of the Partially Active HIS40LYS Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca107 b:27.2 occ:1.00 O B:HOH137 2.2 30.5 1.0 O B:HOH218 2.3 36.6 1.0 O B:HOH122 2.4 19.8 1.0 OD2 B:ASP15 2.5 12.8 1.0 OD1 B:ASP15 2.5 15.6 1.0 O B:HOH216 2.7 30.4 1.0 CG B:ASP15 2.8 14.0 1.0 O B:HOH217 2.9 27.7 1.0 O B:HOH220 3.9 29.9 1.0 CB B:ASP15 4.2 11.5 1.0 O B:HOH226 4.4 38.1 1.0 OG B:SER12 4.5 11.0 1.0 O B:HOH219 4.5 55.8 1.0 O B:HOH117 4.7 12.9 1.0 O B:CYS10 4.9 13.3 1.0

I.Zegers, P.Verhelst, H.W.Choe, J.Steyaert, U.Heinemann, W.Saenger, L.Wyns. Role of Histidine-40 in Ribonuclease T1 Catalysis: Three-Dimensionalstructures of the Partially Active HIS40LYS Mutant. Biochemistry V. 31 11317 1992.
ISSN: ISSN 0006-2960
PubMed: 1445870
DOI: 10.1021/BI00161A009