Calcium in PDB 2aef: Crystal Structures of the Mthk Rck Domain in CA2+ Bound Form

The structure of Crystal Structures of the Mthk Rck Domain in CA2+ Bound Form, PDB code: 2aef was solved by J.Dong, N.Shi, I.Berke, L.Chen, Y.Jiang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.20 / 1.70
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	57.979, 37.908, 96.652, 90.00, 95.49, 90.00
R / Rfree (%)	22.8 / 26.8

The binding sites of Calcium atom in the Crystal Structures of the Mthk Rck Domain in CA2+ Bound Form (pdb code 2aef). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structures of the Mthk Rck Domain in CA2+ Bound Form, PDB code: 2aef:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structures of the Mthk Rck Domain in CA2+ Bound Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structures of the Mthk Rck Domain in CA2+ Bound Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca602 b:28.2 occ:1.00 OE2 A:GLU210 2.3 24.9 1.0 OE2 A:GLU212 2.3 42.7 1.0 OD1 A:ASP184 2.3 29.0 1.0 O A:HOH757 2.3 30.0 1.0 O A:HOH716 2.5 36.6 1.0 O A:HOH698 2.6 39.2 1.0 OD2 A:ASP184 2.7 27.1 1.0 CG A:ASP184 2.8 21.1 1.0 CD A:GLU210 3.3 25.1 1.0 O A:HOH637 3.3 16.7 1.0 CD A:GLU212 3.4 38.0 1.0 OE1 A:GLU212 3.8 44.7 1.0 OE1 A:GLU210 4.0 25.9 1.0 OG A:SER124 4.1 29.9 1.0 CG A:GLU210 4.1 18.8 1.0 CB A:SER124 4.2 24.3 1.0 CB A:ASP184 4.4 17.6 1.0 CB A:GLU210 4.6 16.5 1.0 CG A:GLU212 4.7 36.5 1.0 OG A:SER126 4.7 23.4 1.0 CE2 B:PHE232 4.7 31.4 1.0 O A:HOH618 4.8 28.1 1.0

Calcium binding site 2 out of 2 in the Crystal Structures of the Mthk Rck Domain in CA2+ Bound Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structures of the Mthk Rck Domain in CA2+ Bound Form within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca601 b:29.6 occ:1.00 OE2 B:GLU210 2.3 22.3 1.0 O B:HOH651 2.3 32.7 1.0 O B:HOH750 2.4 33.8 1.0 OE2 B:GLU212 2.4 35.1 1.0 O B:HOH742 2.5 31.7 1.0 OD2 B:ASP184 2.6 24.8 1.0 OD1 B:ASP184 2.7 22.8 1.0 CG B:ASP184 3.0 22.6 1.0 CD B:GLU210 3.4 24.6 1.0 CD B:GLU212 3.4 45.1 1.0 O B:HOH620 3.6 21.4 1.0 OE1 B:GLU212 3.8 34.6 1.0 OE1 B:GLU210 4.1 24.9 1.0 OG B:SER126 4.2 24.8 1.0 OG B:SER124 4.3 20.1 1.0 CG B:GLU210 4.4 21.5 1.0 CB B:SER124 4.4 34.7 1.0 CE2 A:PHE232 4.5 35.5 1.0 CB B:ASP184 4.5 20.8 1.0 O B:HOH639 4.7 46.3 1.0 CG B:GLU212 4.8 35.6 1.0 CB B:SER126 4.8 22.8 1.0 CD2 A:PHE232 4.8 28.9 1.0 CB B:GLU210 4.9 21.0 1.0 O B:HOH614 4.9 32.5 1.0 O B:LEU185 5.0 25.0 1.0

J.Dong, N.Shi, I.Berke, L.Chen, Y.Jiang. Structures of the Mthk Rck Domain and the Effect of CA2+ on Gating Ring Stability J.Biol.Chem. V. 280 41716 2005.
ISSN: ISSN 0021-9258
PubMed: 16227203
DOI: 10.1074/JBC.M508144200