Calcium in PDB 2aii: Wild-Type Formylglycine Generating Enzyme Reacted with Iodoacetamide

The structure of Wild-Type Formylglycine Generating Enzyme Reacted with Iodoacetamide, PDB code: 2aii was solved by D.Roeser, M.G.Rudolph, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.48 / 1.54
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	62.058, 109.788, 43.461, 90.00, 90.00, 90.00
R / Rfree (%)	15 / 17.6

The binding sites of Calcium atom in the Wild-Type Formylglycine Generating Enzyme Reacted with Iodoacetamide (pdb code 2aii). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Wild-Type Formylglycine Generating Enzyme Reacted with Iodoacetamide, PDB code: 2aii:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Wild-Type Formylglycine Generating Enzyme Reacted with Iodoacetamide


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Wild-Type Formylglycine Generating Enzyme Reacted with Iodoacetamide within 5.0Å range: probe atom residue distance (Å) B Occ X:Ca1001 b:6.0 occ:1.00 OE2 X:GLU130 2.3 6.2 1.0 O X:ASN293 2.3 7.2 1.0 OE2 X:GLU300 2.3 6.7 1.0 O X:ALA298 2.3 6.1 1.0 O X:GLY296 2.4 7.3 1.0 O X:HOH2002 2.4 6.0 1.0 CD X:GLU300 3.3 7.2 1.0 CD X:GLU130 3.4 7.2 1.0 C X:ALA298 3.5 6.7 1.0 C X:ASN293 3.6 6.1 1.0 C X:GLY296 3.6 6.8 1.0 N X:ALA298 3.7 6.2 1.0 CG X:GLU300 3.7 7.1 1.0 CG X:GLU130 3.9 6.1 1.0 O X:ILE294 3.9 7.5 1.0 C X:ILE294 4.1 6.8 1.0 CA X:ALA298 4.1 6.3 1.0 O X:GLY332 4.3 7.3 1.0 CA X:ILE294 4.3 6.8 1.0 CB X:ASN297 4.3 6.4 1.0 OE1 X:GLU130 4.4 6.7 1.0 N X:GLY296 4.4 6.5 1.0 N X:ILE294 4.4 6.2 1.0 C X:VAL295 4.4 6.7 1.0 CB X:ASN293 4.4 5.3 1.0 OE1 X:GLU300 4.5 5.9 1.0 C X:ASN297 4.5 6.4 1.0 NH2 X:ARG364 4.5 6.1 1.0 N X:ASN297 4.5 5.8 1.0 CA X:ASN293 4.5 6.4 1.0 CA X:GLY296 4.6 6.5 1.0 N X:TRP299 4.6 6.7 1.0 CA X:ASN297 4.6 6.3 1.0 O X:VAL295 4.6 7.0 1.0 N X:VAL295 4.7 7.0 1.0 CB X:ALA298 4.7 6.5 1.0 CA X:VAL295 4.9 7.4 1.0 C X:TRP299 4.9 6.5 1.0 CA X:TRP299 4.9 6.9 1.0

Calcium binding site 2 out of 2 in the Wild-Type Formylglycine Generating Enzyme Reacted with Iodoacetamide


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Wild-Type Formylglycine Generating Enzyme Reacted with Iodoacetamide within 5.0Å range: probe atom residue distance (Å) B Occ X:Ca1002 b:6.7 occ:1.00 OD1 X:ASN259 2.3 6.6 1.0 O X:PHE275 2.3 7.2 1.0 O X:ILE260 2.3 6.6 1.0 O X:HOH2014 2.4 8.4 1.0 O X:HOH2015 2.4 8.6 1.0 OD2 X:ASP273 2.5 6.7 1.0 OD1 X:ASP273 2.5 7.6 1.0 CG X:ASP273 2.8 6.9 1.0 C X:ILE260 3.5 7.0 1.0 CG X:ASN259 3.5 6.4 1.0 C X:PHE275 3.5 7.2 1.0 N X:ILE260 3.8 7.1 1.0 OE1 X:GLN262 4.1 7.5 1.0 C X:ASN259 4.2 6.3 1.0 CA X:ILE260 4.3 6.7 1.0 CA X:ASN259 4.3 6.1 1.0 CB X:ASP273 4.3 6.9 1.0 CA X:PHE275 4.3 6.7 1.0 N X:PHE275 4.4 6.9 1.0 ND2 X:ASN259 4.4 7.0 1.0 NE2 X:GLN262 4.4 8.5 1.0 CB X:PHE275 4.4 7.3 1.0 CB X:ASN259 4.5 6.2 1.0 O X:TYR334 4.5 8.5 1.0 N X:GLN276 4.5 8.0 1.0 N X:TRP261 4.5 7.4 1.0 O X:GLN276 4.6 8.8 1.0 ND2 X:ASN269 4.6 10.1 1.0 OD1 X:ASN269 4.6 12.8 1.0 CA X:GLN276 4.6 8.4 1.0 CD X:GLN262 4.6 7.6 1.0 CA X:TRP261 4.7 8.0 1.0 C X:GLN276 4.8 7.4 1.0 O X:GLY277 4.9 8.4 1.0 CB X:TYR334 4.9 8.4 1.0 O X:ASN259 4.9 6.8 1.0

D.Roeser, A.Preusser-Kunze, B.Schmidt, K.Gasow, J.G.Wittmann, T.Dierks, K.Von Figura, M.G.Rudolph. A General Binding Mechanism For All Human Sulfatases By the Formylglycine-Generating Enzyme Proc.Natl.Acad.Sci.Usa V. 103 81 2006.
ISSN: ISSN 0027-8424
PubMed: 16368756
DOI: 10.1073/PNAS.0507592102