Calcium in PDB 2asm: Structure of Rabbit Actin in Complex with Reidispongiolide A

The structure of Structure of Rabbit Actin in Complex with Reidispongiolide A, PDB code: 2asm was solved by J.S.Allingham, A.Zampella, M.V.D'auria, I.Rayment, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.00 / 1.60
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	171.181, 54.694, 40.674, 90.00, 95.97, 90.00
R / Rfree (%)	16.4 / 18.9

The binding sites of Calcium atom in the Structure of Rabbit Actin in Complex with Reidispongiolide A (pdb code 2asm). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Structure of Rabbit Actin in Complex with Reidispongiolide A, PDB code: 2asm:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Structure of Rabbit Actin in Complex with Reidispongiolide A


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of Rabbit Actin in Complex with Reidispongiolide A within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca400 b:10.3 occ:1.00 O2B A:ATP425 2.3 9.7 1.0 O3G A:ATP425 2.3 9.4 1.0 O A:HOH823 2.4 9.1 1.0 O A:HOH954 2.4 10.5 1.0 O A:HOH827 2.4 9.9 1.0 O A:HOH828 2.5 11.3 1.0 O A:HOH808 2.6 11.6 1.0 PG A:ATP425 3.5 11.2 1.0 PB A:ATP425 3.6 9.8 1.0 O1G A:ATP425 3.9 10.5 1.0 O3B A:ATP425 3.9 10.8 1.0 O A:HOH840 4.2 14.7 1.0 O3A A:ATP425 4.2 9.9 1.0 OE1 A:GLN137 4.2 10.1 1.0 O1A A:ATP425 4.3 10.8 1.0 CA A:GLY13 4.3 12.0 1.0 NZ A:LYS18 4.3 10.6 1.0 CD A:GLN137 4.5 10.1 1.0 O A:HOH800 4.6 14.3 1.0 OD2 A:ASP154 4.6 14.7 1.0 OD1 A:ASP11 4.7 10.1 1.0 PA A:ATP425 4.8 11.4 1.0 OD2 A:ASP11 4.8 10.4 1.0 O1B A:ATP425 4.8 9.9 1.0 OD1 A:ASP154 4.8 19.5 1.0 O A:HOH809 4.8 21.1 1.0 O2G A:ATP425 4.8 11.8 1.0 CG A:GLN137 4.8 7.9 1.0 NE2 A:GLN137 5.0 11.3 1.0

Calcium binding site 2 out of 2 in the Structure of Rabbit Actin in Complex with Reidispongiolide A


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of Rabbit Actin in Complex with Reidispongiolide A within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca600 b:2.0 occ:1.00 O A:HOH779 2.4 14.1 1.0 OE1 A:GLN263 2.4 13.7 1.0 O A:HOH866 2.4 18.8 1.0 O1 A:EDO704 2.5 30.5 1.0 O A:HOH834 2.6 25.6 1.0 O A:HOH846 2.6 29.2 1.0 OG A:SER265 2.6 13.2 1.0 O2 A:EDO704 2.7 32.0 1.0 CD A:GLN263 3.4 11.5 1.0 C2 A:EDO704 3.4 31.5 1.0 C1 A:EDO704 3.4 31.7 1.0 CB A:SER265 3.6 11.9 1.0 NE2 A:GLN263 3.7 11.0 1.0 N A:SER265 4.2 10.9 1.0 O A:SER271 4.4 14.7 1.0 CA A:SER265 4.5 11.0 1.0 O A:HOH824 4.5 16.0 1.0 O A:HOH864 4.6 21.8 1.0 O A:GLU270 4.6 17.5 1.0 O A:HOH850 4.7 30.7 1.0 O A:MET269 4.7 14.2 1.0 CG A:GLN263 4.8 9.6 1.0 CD A:PRO264 5.0 10.0 1.0 C A:GLU270 5.0 16.0 1.0

J.S.Allingham, A.Zampella, M.V.D'auria, I.Rayment. Structures of Microfilament Destabilizing Toxins Bound to Actin Provide Insight Into Toxin Design and Activity Proc.Natl.Acad.Sci.Usa V. 102 14527 2005.
ISSN: ISSN 0027-8424
PubMed: 16192358
DOI: 10.1073/PNAS.0502089102