Calcium in PDB 2bir: Additivity of Substrate Binding in Ribonuclease T1 (Y42A Mutant)

The structure of Additivity of Substrate Binding in Ribonuclease T1 (Y42A Mutant), PDB code: 2bir was solved by J.Doumen, J.Steyaert, S.Loverix, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	49.610, 48.490, 40.610, 90.00, 90.00, 90.00
R / Rfree (%)	21.6 / 25.7

The binding sites of Calcium atom in the Additivity of Substrate Binding in Ribonuclease T1 (Y42A Mutant) (pdb code 2bir). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Additivity of Substrate Binding in Ribonuclease T1 (Y42A Mutant), PDB code: 2bir:

Calcium binding site 1 out of 1 in the Additivity of Substrate Binding in Ribonuclease T1 (Y42A Mutant)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Additivity of Substrate Binding in Ribonuclease T1 (Y42A Mutant) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca200 b:31.1 occ:1.00 OD2 A:ASP15 2.5 17.1 1.0 O A:HOH172 2.5 50.7 1.0 O A:HOH178 2.7 65.3 1.0 O A:HOH131 2.8 23.5 1.0 OD1 A:ASP15 2.9 16.9 1.0 CG A:ASP15 3.1 18.2 1.0 O A:HOH127 4.4 22.9 1.0 OG A:SER12 4.5 4.2 1.0 CB A:ASP15 4.6 10.9 1.0 O A:HOH109 4.6 12.3 1.0 O A:CYS10 4.7 16.8 1.0 O A:HOH146 4.7 39.4 1.0 CA A:GLY65 5.0 22.0 1.0

S.Loverix, J.Doumen, J.Steyaert. Additivity of Protein-Guanine Interactions in Ribonuclease T1. J.Biol.Chem. V. 272 9635 1997.
ISSN: ISSN 0021-9258
PubMed: 9092491
DOI: 10.1074/JBC.272.15.9635