Calcium in PDB 2bl0: Physarum Polycephalum Myosin II Regulatory Domain

All present enzymatic activity of Physarum Polycephalum Myosin II Regulatory Domain:
3.6.1.32;

The structure of Physarum Polycephalum Myosin II Regulatory Domain, PDB code: 2bl0 was solved by J.E.Debreczeni, L.Farkas, V.Harmat, L.Nyitray, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	57.74 / 1.75
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	55.574, 70.858, 97.877, 90.00, 90.00, 90.00
R / Rfree (%)	21.9 / 25.2

The binding sites of Calcium atom in the Physarum Polycephalum Myosin II Regulatory Domain (pdb code 2bl0). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Physarum Polycephalum Myosin II Regulatory Domain, PDB code: 2bl0:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Physarum Polycephalum Myosin II Regulatory Domain


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Physarum Polycephalum Myosin II Regulatory Domain within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1146 b:29.0 occ:1.00 O B:HOH2014 2.2 42.8 1.0 OD1 B:ASP15 2.2 5.9 1.0 O B:LYS21 2.3 6.7 1.0 O B:HOH2016 2.3 38.9 1.0 OD1 B:ASP17 2.4 5.5 1.0 OD1 B:ASP19 2.4 6.6 1.0 CG B:ASP15 3.4 5.5 1.0 CG B:ASP19 3.4 7.2 1.0 C B:LYS21 3.4 7.8 1.0 CG B:ASP17 3.5 10.3 1.0 OD2 B:ASP17 3.8 13.2 1.0 O B:HOH2010 3.9 57.3 1.0 OD2 B:ASP19 3.9 11.2 1.0 N B:LYS21 4.0 7.3 1.0 CA B:ASP15 4.1 6.6 1.0 CA B:LYS21 4.2 8.1 1.0 OD2 B:ASP15 4.2 6.8 1.0 N B:ASP19 4.2 7.2 1.0 CB B:ASP15 4.2 5.7 1.0 N B:VAL22 4.5 7.6 1.0 C B:ASP15 4.5 8.1 1.0 CB B:LYS21 4.5 8.7 1.0 N B:ASP17 4.5 9.6 1.0 CB B:ASP19 4.5 7.2 1.0 N B:ASN18 4.6 7.8 1.0 O B:HOH2021 4.7 35.7 1.0 CA B:VAL22 4.7 7.1 1.0 CB B:ASP17 4.7 9.6 1.0 N B:LYS16 4.7 9.0 1.0 CA B:ASP19 4.7 7.2 1.0 N B:GLY20 4.8 7.8 1.0 C B:ASP17 4.9 8.8 1.0 CA B:ASP17 4.9 9.2 1.0 C B:ASP19 5.0 7.3 1.0

Calcium binding site 2 out of 2 in the Physarum Polycephalum Myosin II Regulatory Domain


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Physarum Polycephalum Myosin II Regulatory Domain within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca1155 b:23.9 occ:1.00 OD1 C:ASP64 2.3 7.3 1.0 O C:LYS70 2.3 4.1 1.0 OD1 C:ASN68 2.4 5.8 1.0 OG1 C:THR66 2.4 5.5 1.0 OE2 C:GLU75 2.4 3.7 1.0 OE1 C:GLU75 2.5 4.1 1.0 CD C:GLU75 2.8 2.5 1.0 CG C:ASN68 3.4 7.5 1.0 C C:LYS70 3.5 4.9 1.0 CG C:ASP64 3.5 8.0 1.0 CB C:THR66 3.7 6.5 1.0 CG2 C:THR66 4.1 5.9 1.0 N C:THR66 4.2 5.9 1.0 ND2 C:ASN68 4.2 7.7 1.0 N C:LYS70 4.3 4.8 1.0 OD2 C:ASP64 4.3 7.2 1.0 N C:ASN68 4.3 7.9 1.0 CG C:GLU75 4.3 4.3 1.0 N C:ILE71 4.4 4.9 1.0 CA C:LYS70 4.4 4.6 1.0 CA C:ASP64 4.4 7.1 1.0 CA C:THR66 4.4 6.5 1.0 CB C:ASN68 4.4 7.2 1.0 CA C:ILE71 4.4 5.2 1.0 CB C:ASP64 4.5 7.2 1.0 N C:GLY67 4.5 8.1 1.0 N C:GLN72 4.6 5.4 1.0 CG C:GLN72 4.6 7.3 1.0 C C:ASP64 4.6 7.0 1.0 OE1 C:GLN72 4.6 12.1 1.0 C C:THR66 4.7 7.3 1.0 CB C:LYS70 4.7 4.8 1.0 CA C:ASN68 4.8 7.5 1.0 N C:ALA65 4.8 7.3 1.0

J.E.Debreczeni, L.Farkas, V.Harmat, C.Hetenyi, I.Hajdu, P.Zavodszky, K.Kohama, L.Nyitray. Structural Evidence For Non-Canonical Binding of CA2+ to A Canonical Ef-Hand of A Conventional Myosin. J.Biol.Chem. V. 280 41458 2005.
ISSN: ISSN 0021-9258
PubMed: 16227209
DOI: 10.1074/JBC.M506315200