Atomistry » Calcium » PDB 2bir-2bw1 » 2br0
Atomistry »
  Calcium »
    PDB 2bir-2bw1 »
      2br0 »

Calcium in PDB 2br0: Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus DPO4. Analysis and Crystal Structures of Multiple Base- Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine

Enzymatic activity of Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus DPO4. Analysis and Crystal Structures of Multiple Base- Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine

All present enzymatic activity of Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus DPO4. Analysis and Crystal Structures of Multiple Base- Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine:
2.7.7.7;

Protein crystallography data

The structure of Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus DPO4. Analysis and Crystal Structures of Multiple Base- Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine, PDB code: 2br0 was solved by A.Irimia, L.V.Loukachevitch, M.Egli, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 29.81 / 2.17
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 93.506, 102.072, 52.532, 90.00, 90.00, 90.00
R / Rfree (%) 23.9 / 26.6

Calcium Binding Sites:

The binding sites of Calcium atom in the Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus DPO4. Analysis and Crystal Structures of Multiple Base- Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine (pdb code 2br0). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus DPO4. Analysis and Crystal Structures of Multiple Base- Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine, PDB code: 2br0:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in 2br0

Go back to Calcium Binding Sites List in 2br0
Calcium binding site 1 out of 3 in the Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus DPO4. Analysis and Crystal Structures of Multiple Base- Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus DPO4. Analysis and Crystal Structures of Multiple Base- Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca2001

b:69.3
occ:1.00
 OE2 A:GLU106 2.2 49.5 1.0
OD1 A:ASP7 2.7 34.9 1.0
CD A:GLU106 3.0 45.8 1.0
OD2 A:ASP105 3.2 32.9 1.0
CG A:GLU106 3.3 42.3 1.0
CA A:CA2002 3.4 33.0 1.0
CG A:ASP7 3.5 33.2 1.0
OD1 A:ASP105 3.7 37.0 1.0
OD2 A:ASP7 3.7 30.0 1.0
O A:HOH2041 3.8 65.2 1.0
CG A:ASP105 3.8 35.0 1.0
OE1 A:GLU106 4.0 49.0 1.0
NZ A:LYS159 4.3 47.6 1.0
C5' A:DG1000 4.4 54.4 1.0
CB A:GLU106 4.7 36.3 1.0
CB A:ASP7 4.7 32.0 1.0
O3' P:DC13 4.9 53.0 1.0
O A:ASP105 4.9 27.7 1.0

Calcium binding site 2 out of 3 in 2br0

Go back to Calcium Binding Sites List in 2br0
Calcium binding site 2 out of 3 in the Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus DPO4. Analysis and Crystal Structures of Multiple Base- Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus DPO4. Analysis and Crystal Structures of Multiple Base- Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca2002

b:33.0
occ:1.00
 OD2 A:ASP105 2.3 32.9 1.0
O A:PHE8 2.4 29.3 1.0
OD2 A:ASP7 2.5 30.0 1.0
CA A:CA2001 3.4 69.3 1.0
CG A:ASP7 3.4 33.2 1.0
CG A:ASP105 3.5 35.0 1.0
C A:PHE8 3.5 30.9 1.0
O A:HOH2006 3.6 32.9 1.0
OD1 A:ASP7 3.7 34.9 1.0
O A:HOH2011 3.7 59.2 1.0
C5' A:DG1000 3.8 54.4 1.0
N A:PHE8 4.1 31.3 1.0
O5' A:DG1000 4.2 56.7 1.0
C A:ASP7 4.3 31.5 1.0
OD1 A:ASP105 4.3 37.0 1.0
N A:TYR10 4.4 29.7 1.0
CA A:PHE8 4.4 30.5 1.0
CB A:ASP105 4.4 34.1 1.0
NZ A:LYS159 4.4 47.6 1.0
N A:ASP9 4.5 30.6 1.0
CA A:ASP9 4.5 30.7 1.0
C A:ASP9 4.6 29.7 1.0
O A:ASP105 4.6 27.7 1.0
CB A:ASP7 4.7 32.0 1.0
O A:ASP7 4.7 32.2 1.0
N A:PHE11 4.7 26.8 1.0
CA A:ASP7 4.8 30.5 1.0
CB A:PHE8 4.9 28.8 1.0

Calcium binding site 3 out of 3 in 2br0

Go back to Calcium Binding Sites List in 2br0
Calcium binding site 3 out of 3 in the Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus DPO4. Analysis and Crystal Structures of Multiple Base- Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus DPO4. Analysis and Crystal Structures of Multiple Base- Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca2003

b:50.8
occ:1.00
 O A:ALA181 2.5 41.9 1.0
O P:HOH2004 2.6 58.6 1.0
O A:HOH2053 2.6 49.6 1.0
O P:HOH2013 2.7 48.9 1.0
O P:HOH2014 2.8 46.8 1.0
O A:ILE186 2.9 38.2 1.0
C A:ALA181 3.4 41.8 1.0
C A:ILE186 4.1 38.4 1.0
CA A:ALA181 4.2 42.0 1.0
N A:ASP182 4.3 42.7 1.0
CB A:ALA181 4.4 40.9 1.0
CA A:ASP182 4.5 42.6 1.0
OP1 P:DC13 4.5 41.5 1.0
CA A:GLY187 4.5 40.7 1.0
OP2 P:DC13 4.6 42.4 1.0
O A:VAL183 4.8 38.8 1.0
N A:GLY187 4.8 39.1 1.0
C A:GLY187 4.9 42.1 1.0
OD1 A:ASP182 4.9 55.6 1.0
C A:ASP182 5.0 40.8 1.0

Reference:

H.Zang, A.K.Goodenough, J.Y.Choi, A.Irimia, L.V.Loukachevitch, I.D.Kozekov, K.C.Angel, C.J.Rizzo, M.Egli, F.P.Guengerich. Dna Adduct Bypass Polymerization By Sulfolobus Solfataricus Dna Polymerase DPO4: Analysis and Crystal Structures of Multiple Base Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine. J.Biol.Chem. V. 280 29750 2005.
ISSN: ISSN 0021-9258
PubMed: 15965231
DOI: 10.1074/JBC.M504756200
Page generated: Sat Dec 12 03:32:48 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy