Calcium in PDB 2bsp: Bacillus Subtilis Pectate Lyase R279K Mutant

All present enzymatic activity of Bacillus Subtilis Pectate Lyase R279K Mutant:
4.2.2.2;

The structure of Bacillus Subtilis Pectate Lyase R279K Mutant, PDB code: 2bsp was solved by R.Pickersgill, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	51.430, 69.710, 60.230, 90.00, 112.65, 90.00
R / Rfree (%)	19.6 / 22.5

The binding sites of Calcium atom in the Bacillus Subtilis Pectate Lyase R279K Mutant (pdb code 2bsp). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Bacillus Subtilis Pectate Lyase R279K Mutant, PDB code: 2bsp:

Calcium binding site 1 out of 1 in the Bacillus Subtilis Pectate Lyase R279K Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Bacillus Subtilis Pectate Lyase R279K Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca400 b:6.3 occ:1.00 OD2 A:ASP223 2.8 10.0 1.0 OD2 A:ASP227 2.8 11.3 1.0 OD1 A:ASP184 2.9 6.0 1.0 OD2 A:ASP184 2.9 7.5 1.0 CG A:ASP184 3.2 6.9 1.0 O A:HOH543 3.3 47.1 1.0 O A:HOH484 3.3 59.3 1.0 CG A:ASP227 3.8 9.1 1.0 CG A:ASP223 4.0 8.6 1.0 CB A:ASP227 4.1 7.9 1.0 O A:HOH513 4.3 19.5 1.0 N A:GLY224 4.4 5.3 1.0 CB A:ASP223 4.6 7.0 1.0 O A:HOH609 4.6 27.5 1.0 CB A:ASP184 4.7 5.6 1.0 CA A:GLY224 4.8 3.8 1.0 O A:HOH529 4.8 24.5 1.0 OD1 A:ASP223 4.9 9.9 1.0 OD1 A:ASP227 4.9 8.3 1.0

R.Pickersgill, K.Worboys, M.Scott, N.Cummings, A.Cooper, J.Jenkins, D.Smith. The Conserved Arginine Proximal to the Essential Calcium of Bacillus Subtilis Pectate Lyase Stabilizes the Transition State To Be Published.