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Calcium in PDB 2c4l: Structure of Neuraminidase Subtype N9 Complexed with 30 Mm Sialic Acid (Nana, NEU5AC), Crystal Soaked For 24 Hours at 291 K and Finally Backsoaked For 30 Min in A Cryoprotectant Solution Which Did Not Contain NEU5AC

Enzymatic activity of Structure of Neuraminidase Subtype N9 Complexed with 30 Mm Sialic Acid (Nana, NEU5AC), Crystal Soaked For 24 Hours at 291 K and Finally Backsoaked For 30 Min in A Cryoprotectant Solution Which Did Not Contain NEU5AC

All present enzymatic activity of Structure of Neuraminidase Subtype N9 Complexed with 30 Mm Sialic Acid (Nana, NEU5AC), Crystal Soaked For 24 Hours at 291 K and Finally Backsoaked For 30 Min in A Cryoprotectant Solution Which Did Not Contain NEU5AC:
3.2.1.18;

Protein crystallography data

The structure of Structure of Neuraminidase Subtype N9 Complexed with 30 Mm Sialic Acid (Nana, NEU5AC), Crystal Soaked For 24 Hours at 291 K and Finally Backsoaked For 30 Min in A Cryoprotectant Solution Which Did Not Contain NEU5AC, PDB code: 2c4l was solved by E.Rudino-Pinera, P.Tunnah, S.J.Crennell, R.G.Webster, W.G.Laver, E.F.Garman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 42.70 / 2.15
Space group I 4 3 2
Cell size a, b, c (Å), α, β, γ (°) 181.178, 181.178, 181.178, 90.00, 90.00, 90.00
R / Rfree (%) 15.5 / 19.6

Calcium Binding Sites:

The binding sites of Calcium atom in the Structure of Neuraminidase Subtype N9 Complexed with 30 Mm Sialic Acid (Nana, NEU5AC), Crystal Soaked For 24 Hours at 291 K and Finally Backsoaked For 30 Min in A Cryoprotectant Solution Which Did Not Contain NEU5AC (pdb code 2c4l). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Structure of Neuraminidase Subtype N9 Complexed with 30 Mm Sialic Acid (Nana, NEU5AC), Crystal Soaked For 24 Hours at 291 K and Finally Backsoaked For 30 Min in A Cryoprotectant Solution Which Did Not Contain NEU5AC, PDB code: 2c4l:

Calcium binding site 1 out of 1 in 2c4l

Go back to Calcium Binding Sites List in 2c4l
Calcium binding site 1 out of 1 in the Structure of Neuraminidase Subtype N9 Complexed with 30 Mm Sialic Acid (Nana, NEU5AC), Crystal Soaked For 24 Hours at 291 K and Finally Backsoaked For 30 Min in A Cryoprotectant Solution Which Did Not Contain NEU5AC


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of Neuraminidase Subtype N9 Complexed with 30 Mm Sialic Acid (Nana, NEU5AC), Crystal Soaked For 24 Hours at 291 K and Finally Backsoaked For 30 Min in A Cryoprotectant Solution Which Did Not Contain NEU5AC within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca1483

b:15.4
occ:1.00
O A:ASP293 2.5 13.4 1.0
O A:GLY297 2.5 16.6 1.0
O A:ASN347 2.7 15.6 1.0
OD2 A:ASP324 2.7 14.2 1.0
O A:HOH2145 2.9 12.0 1.0
O A:HOH2138 2.9 14.9 1.0
CG A:ASP324 3.5 14.4 1.0
C A:ASP293 3.6 13.1 1.0
C A:GLY297 3.6 16.2 1.0
C A:ASN347 3.8 15.2 1.0
CA A:GLY348 3.9 12.7 1.0
CA A:ASN294 4.0 14.4 1.0
O A:HOH2136 4.0 10.5 1.0
O A:ASN294 4.1 15.9 1.0
CB A:ASP324 4.2 13.2 1.0
C A:ASN294 4.2 15.4 1.0
N A:ASN294 4.3 13.9 1.0
OD1 A:ASP324 4.3 14.7 1.0
N A:GLY297 4.3 17.2 1.0
N A:GLY348 4.4 14.7 1.0
N A:SER298 4.5 15.5 1.0
CA A:GLY297 4.5 16.3 1.0
CA A:SER298 4.6 15.3 1.0
CA A:ASP293 4.7 13.0 1.0
N A:ASP293 4.8 12.4 1.0
OG A:SER298 4.9 14.7 1.0
C A:GLN296 5.0 17.2 1.0
N A:ASN347 5.0 15.6 1.0

Reference:

E.Rudino-Pinera, S.J.Crennell, R.G.Webster, W.G.Laver, E.F.Garman. The Crystal Structure of Influenza Type A Virus Neuraminidase of the N6 Subtype at 1.85 A Resolution To Be Published.
Page generated: Sat Dec 12 03:33:39 2020

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