Calcium in PDB 2cn7: Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant

All present enzymatic activity of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant:
1.16.3.1;

The structure of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, PDB code: 2cn7 was solved by L.Toussaint, R.R.Crichton, J.P.Declercq, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	104.83 / 1.75
Space group	F 4 3 2
Cell size a, b, c (Å), α, β, γ (°)	181.207, 181.207, 181.207, 90.00, 90.00, 90.00
R / Rfree (%)	18.4 / 20.4

The binding sites of Calcium atom in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant (pdb code 2cn7). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant, PDB code: 2cn7:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca210 b:43.4 occ:0.50 OE1 A:GLN86 2.6 26.5 1.0 O A:HOH2110 3.8 32.5 1.0 CD A:GLN86 3.8 24.2 1.0 NE2 A:GLN86 4.4 26.4 1.0 CG A:GLN86 5.0 21.3 1.0

Calcium binding site 2 out of 3 in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca211 b:35.8 occ:0.33 O A:HOH2152 2.3 33.5 1.0 OE1 A:GLU134 2.4 27.4 1.0 CD A:GLU134 3.6 25.6 1.0 O A:HOH2153 3.8 42.0 1.0 OD1 A:ASP131 4.2 23.9 1.0 OE2 A:GLU134 4.2 26.6 1.0 CB A:GLU134 4.7 24.0 1.0 CG A:GLU134 4.8 25.5 1.0 CA A:ASP131 4.9 20.5 1.0 CB A:ASP131 5.0 21.4 1.0

Calcium binding site 3 out of 3 in the Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Recombinant Human H Ferritin, K86Q, E27D and E107D Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca218 b:20.9 occ:1.00 OE1 A:GLN58 2.3 20.8 1.0 OE1 A:GLU61 2.4 25.2 1.0 O A:HOH2087 2.4 23.0 1.0 O A:HOH2126 2.4 23.1 1.0 O A:HOH2081 2.4 24.4 1.0 O A:HOH2083 2.5 22.1 1.0 O A:HOH2052 2.5 23.8 1.0 CD A:GLN58 3.5 18.9 1.0 CD A:GLU61 3.5 26.9 1.0 O A:HOH2085 4.2 37.8 1.0 CB A:ALA144 4.2 16.4 1.0 OE2 A:GLU62 4.2 24.6 1.0 CG A:GLU61 4.2 25.7 1.0 CG A:GLN58 4.3 17.3 1.0 O A:HOH2160 4.3 19.6 1.0 O A:HOH2086 4.3 24.2 1.0 CB A:GLN58 4.3 16.6 1.0 OE2 A:GLU61 4.4 32.1 1.0 CA A:GLN58 4.5 16.6 1.0 OD2 A:ASP107 4.5 18.7 1.0 NE2 A:GLN58 4.5 20.5 1.0 CB A:GLU61 4.6 21.1 1.0 O A:GLN58 4.9 16.2 1.0 OH A:TYR34 4.9 19.9 1.0

L.Toussaint, L.Bertrand, L.Hue, R.R.Crichton, J.P.Declercq. High-Resolution X-Ray Structures of Human Apoferritin H-Chain Mutants Correlated with Their Activity and Metal-Binding Sites. J.Mol.Biol. V. 365 440 2007.
ISSN: ISSN 0022-2836
PubMed: 17070541
DOI: 10.1016/J.JMB.2006.10.010