Calcium in PDB 2e8y: Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168

All present enzymatic activity of Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168:
3.2.1.41;

The structure of Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168, PDB code: 2e8y was solved by B.Mikami, D.Malle, S.Utsumi, H.Iwamoto, Y.Katsuya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.11
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	70.562, 127.682, 189.251, 90.00, 90.00, 90.00
R / Rfree (%)	20.1 / 23.8

The binding sites of Calcium atom in the Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 (pdb code 2e8y). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168, PDB code: 2e8y:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca741 b:19.1 occ:1.00 O A:PHE276 2.3 16.6 1.0 O A:HOH769 2.4 11.3 1.0 OD2 A:ASP275 2.4 14.9 1.0 OE2 A:GLU281 2.5 18.8 1.0 O A:HOH779 2.5 15.7 1.0 OE2 A:GLU301 2.5 14.9 1.0 O A:HOH858 2.6 22.9 1.0 CD A:GLU301 3.3 19.3 1.0 OE1 A:GLU301 3.5 18.5 1.0 C A:PHE276 3.5 16.4 1.0 CG A:ASP275 3.5 12.3 1.0 CD A:GLU281 3.6 18.0 1.0 N A:PHE276 3.7 14.9 1.0 O A:HOH778 4.0 14.9 1.0 OD1 A:ASP275 4.0 17.1 1.0 CG A:GLU281 4.1 18.7 1.0 CA A:PHE276 4.2 16.6 1.0 N A:GLY302 4.4 17.8 1.0 O A:VAL279 4.5 21.2 1.0 N A:ALA277 4.5 19.5 1.0 C A:ASP275 4.6 15.9 1.0 OE1 A:GLU281 4.6 18.4 1.0 CD2 A:PHE276 4.6 15.3 1.0 CA A:ASP275 4.7 16.5 1.0 CB A:ASP275 4.7 14.9 1.0 CA A:ALA277 4.7 19.0 1.0 CG A:GLU301 4.8 18.0 1.0 CD1 A:LEU295 4.8 20.1 1.0 OD1 A:ASN188 4.9 24.5 1.0 CA A:ASN188 4.9 19.6 1.0 CA A:GLU301 4.9 18.1 1.0 CG A:PHE276 5.0 16.4 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca742 b:18.1 occ:1.00 OD2 B:ASP275 2.3 12.7 1.0 O B:PHE276 2.4 11.2 1.0 O B:HOH828 2.4 16.9 1.0 OE2 B:GLU281 2.4 17.7 1.0 O B:HOH874 2.5 22.8 1.0 OE2 B:GLU301 2.5 12.9 1.0 O B:HOH813 2.6 16.3 1.0 CD B:GLU301 3.3 15.1 1.0 OE1 B:GLU301 3.5 14.2 1.0 CG B:ASP275 3.5 14.9 1.0 CD B:GLU281 3.5 18.9 1.0 C B:PHE276 3.5 14.3 1.0 N B:PHE276 3.8 15.8 1.0 OD1 B:ASP275 4.0 15.4 1.0 O B:HOH772 4.0 14.4 1.0 CG B:GLU281 4.1 19.2 1.0 CA B:PHE276 4.3 13.5 1.0 N B:GLY302 4.4 16.4 1.0 O B:VAL279 4.4 19.0 1.0 OE1 B:GLU281 4.6 15.6 1.0 N B:ALA277 4.6 15.5 1.0 C B:ASP275 4.6 16.9 1.0 O B:HOH967 4.6 26.9 1.0 CB B:ASP275 4.7 15.2 1.0 OD1 B:ASN188 4.7 19.2 1.0 CA B:ASP275 4.7 16.8 1.0 CD2 B:PHE276 4.7 12.9 1.0 CG B:GLU301 4.8 15.1 1.0 CD1 B:LEU295 4.8 21.2 1.0 CA B:ALA277 4.8 15.8 1.0 CA B:ASN188 4.8 18.1 1.0 CA B:GLU301 4.9 16.3 1.0 CG B:PHE276 5.0 12.4 1.0

D.Malle, H.Iwamoto, Y.Katsuya, S.Utsumi, B.Mikami. Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 in Complex with Maltose and Alpha-Cyclodextrin To Be Published.