Calcium in PDB 2e9b: Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 Complexed with Maltose

All present enzymatic activity of Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 Complexed with Maltose:
3.2.1.41;

The structure of Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 Complexed with Maltose, PDB code: 2e9b was solved by B.Mikami, D.Malle, S.Utsumi, H.Iwamoto, Y.Katsuya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.97 / 2.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	71.031, 129.178, 192.060, 90.00, 90.00, 90.00
R / Rfree (%)	19.4 / 24.2

The binding sites of Calcium atom in the Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 Complexed with Maltose (pdb code 2e9b). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 Complexed with Maltose, PDB code: 2e9b:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 Complexed with Maltose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 Complexed with Maltose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca741 b:38.6 occ:1.00 O A:HOH1121 2.3 27.8 1.0 O A:PHE276 2.4 25.4 1.0 O A:HOH1090 2.4 27.8 1.0 OD2 A:ASP275 2.4 23.1 1.0 OE2 A:GLU281 2.4 36.8 1.0 OE2 A:GLU301 2.4 26.1 1.0 CD A:GLU301 3.1 28.1 1.0 OE1 A:GLU301 3.1 28.2 1.0 CD A:GLU281 3.4 37.0 1.0 CG A:ASP275 3.4 22.5 1.0 C A:PHE276 3.5 25.1 1.0 N A:PHE276 3.8 21.0 1.0 CG A:GLU281 3.9 34.5 1.0 OD1 A:ASP275 3.9 20.1 1.0 O A:HOH848 4.2 23.9 1.0 CA A:PHE276 4.3 24.6 1.0 OE1 A:GLU281 4.3 39.8 1.0 N A:GLY302 4.4 24.2 1.0 CG A:GLU301 4.5 27.8 1.0 O A:VAL279 4.5 31.4 1.0 N A:ALA277 4.6 27.3 1.0 C A:ASP275 4.6 24.0 1.0 CB A:ASP275 4.7 22.5 1.0 CA A:ASP275 4.7 24.2 1.0 CD2 A:PHE276 4.8 24.9 1.0 CA A:ALA277 4.8 27.8 1.0 CA A:GLU301 4.9 25.3 1.0 CD1 A:LEU295 4.9 25.6 1.0 CG A:PHE276 5.0 25.2 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 Complexed with Maltose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 Complexed with Maltose within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca742 b:38.7 occ:1.00 O B:HOH1208 2.3 29.3 1.0 OD2 B:ASP275 2.3 20.9 1.0 OE2 B:GLU281 2.4 37.6 1.0 O B:HOH1207 2.4 29.9 1.0 O B:PHE276 2.4 22.3 1.0 OE2 B:GLU301 2.5 25.7 1.0 CD B:GLU301 3.3 25.1 1.0 O B:HOH1273 3.3 32.4 1.0 CG B:ASP275 3.4 24.5 1.0 OE1 B:GLU301 3.4 26.9 1.0 CD B:GLU281 3.5 38.4 1.0 C B:PHE276 3.6 24.4 1.0 N B:PHE276 3.7 24.4 1.0 OD1 B:ASP275 3.9 26.1 1.0 CG B:GLU281 4.1 37.2 1.0 O B:HOH980 4.2 25.1 1.0 CA B:PHE276 4.3 23.8 1.0 N B:GLY302 4.5 27.6 1.0 OE1 B:GLU281 4.5 38.3 1.0 O B:VAL279 4.5 30.8 1.0 C B:ASP275 4.6 26.1 1.0 CB B:ASP275 4.7 24.9 1.0 N B:ALA277 4.7 25.7 1.0 CD2 B:PHE276 4.7 21.0 1.0 CA B:ASP275 4.7 25.2 1.0 CG B:GLU301 4.7 25.3 1.0 CD1 B:LEU295 4.9 31.0 1.0 CA B:ALA277 4.9 27.4 1.0 CG B:PHE276 5.0 22.6 1.0 CA B:GLU301 5.0 23.8 1.0 OD1 B:ASN188 5.0 27.4 1.0

D.Malle, H.Iwamoto, Y.Katsuya, S.Utsumi, B.Mikami. Crystal Structure of Pullulanase Type I From Bacillus Subtilis Str. 168 in Complex with Maltose and Alpha-Cyclodextrin To Be Published.