Calcium in PDB 2ezv: Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna.

All present enzymatic activity of Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna.:
3.1.21.4;

The structure of Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna., PDB code: 2ezv was solved by A.K.Aggarwal, E.S.Vanamee, H.Viadiu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.40
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	85.600, 85.600, 202.400, 90.00, 90.00, 120.00
R / Rfree (%)	22.4 / 26.5

The binding sites of Calcium atom in the Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna. (pdb code 2ezv). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna., PDB code: 2ezv:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna. within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca270 b:36.6 occ:1.00 O A:ALA101 2.4 17.9 1.0 OD1 A:ASP100 2.4 19.2 1.0 OD2 A:ASP79 2.4 23.7 1.0 CG A:ASP100 3.2 20.0 1.0 CG A:ASP79 3.2 23.0 1.0 OD1 A:ASP79 3.4 23.6 1.0 C A:ALA101 3.5 17.3 1.0 OD2 A:ASP100 3.6 18.4 1.0 N A:ALA101 3.7 15.2 1.0 CA A:ALA101 4.1 16.6 1.0 O A:HOH324 4.2 19.2 1.0 OE2 A:GLU55 4.3 26.9 1.0 CD A:LYS102 4.4 18.7 1.0 CB A:ASP100 4.4 17.2 1.0 NE2 A:GLN116 4.4 24.6 1.0 C A:ASP100 4.5 15.2 1.0 NZ A:LYS102 4.5 16.7 1.0 CE A:LYS102 4.5 18.4 1.0 CB A:ALA101 4.6 15.6 1.0 N A:LYS102 4.6 18.0 1.0 CB A:ASP79 4.7 22.6 1.0 O A:HOH291 4.7 17.3 1.0 CA A:ASP100 4.7 15.6 1.0 OP1 G:DT33 4.9 42.7 1.0 OP1 G:DG34 4.9 37.5 1.0 CA A:LYS102 5.0 17.9 1.0 CG A:GLU55 5.0 23.5 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna. within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca270 b:61.3 occ:1.00 OD1 B:ASP100 2.6 32.1 1.0 OD2 B:ASP79 2.6 28.5 1.0 O B:ALA101 2.8 22.0 1.0 CG B:ASP100 3.2 31.4 1.0 OD2 B:ASP100 3.2 33.0 1.0 CG B:ASP79 3.5 27.4 1.0 OD1 B:ASP79 3.6 30.2 1.0 C B:ALA101 3.9 22.4 1.0 OP1 F:DA13 4.0 66.3 1.0 N B:ALA101 4.0 19.6 1.0 OE2 B:GLU55 4.2 28.0 1.0 NZ B:LYS102 4.2 32.0 1.0 NE2 B:GLN116 4.3 31.1 1.0 CD B:LYS102 4.3 29.9 1.0 O B:HOH292 4.4 28.4 1.0 CB B:ASP100 4.5 25.5 1.0 CE B:LYS102 4.5 31.0 1.0 OP2 F:DA13 4.5 66.0 1.0 CA B:ALA101 4.5 21.0 1.0 P F:DA13 4.6 66.7 1.0 C B:ASP100 4.7 21.4 1.0 O5' F:DA13 4.8 64.6 1.0 CA B:ASP100 4.8 22.5 1.0 CB B:ASP79 4.9 24.5 1.0 N B:LYS102 5.0 23.8 1.0

E.S.Vanamee, H.Viadiu, R.Kucera, L.Dorner, S.Picone, I.Schildkraut, A.K.Aggarwal. A View of Consecutive Binding Events From Structures of Tetrameric Endonuclease Sfii Bound to Dna Embo J. V. 24 4198 2005.
ISSN: ISSN 0261-4189
PubMed: 16308566
DOI: 10.1038/SJ.EMBOJ.7600880