Atomistry » Calcium » PDB 2eex-2f3z » 2ezv
Atomistry »
  Calcium »
    PDB 2eex-2f3z »
      2ezv »

Calcium in PDB 2ezv: Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna.

Enzymatic activity of Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna.

All present enzymatic activity of Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna.:
3.1.21.4;

Protein crystallography data

The structure of Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna., PDB code: 2ezv was solved by A.K.Aggarwal, E.S.Vanamee, H.Viadiu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 2.40
Space group P 32 2 1
Cell size a, b, c (Å), α, β, γ (°) 85.600, 85.600, 202.400, 90.00, 90.00, 120.00
R / Rfree (%) 22.4 / 26.5

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna. (pdb code 2ezv). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna., PDB code: 2ezv:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 2ezv

Go back to Calcium Binding Sites List in 2ezv
Calcium binding site 1 out of 2 in the Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna. within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca270

b:36.6
occ:1.00
O A:ALA101 2.4 17.9 1.0
OD1 A:ASP100 2.4 19.2 1.0
OD2 A:ASP79 2.4 23.7 1.0
CG A:ASP100 3.2 20.0 1.0
CG A:ASP79 3.2 23.0 1.0
OD1 A:ASP79 3.4 23.6 1.0
C A:ALA101 3.5 17.3 1.0
OD2 A:ASP100 3.6 18.4 1.0
N A:ALA101 3.7 15.2 1.0
CA A:ALA101 4.1 16.6 1.0
O A:HOH324 4.2 19.2 1.0
OE2 A:GLU55 4.3 26.9 1.0
CD A:LYS102 4.4 18.7 1.0
CB A:ASP100 4.4 17.2 1.0
NE2 A:GLN116 4.4 24.6 1.0
C A:ASP100 4.5 15.2 1.0
NZ A:LYS102 4.5 16.7 1.0
CE A:LYS102 4.5 18.4 1.0
CB A:ALA101 4.6 15.6 1.0
N A:LYS102 4.6 18.0 1.0
CB A:ASP79 4.7 22.6 1.0
O A:HOH291 4.7 17.3 1.0
CA A:ASP100 4.7 15.6 1.0
OP1 G:DT33 4.9 42.7 1.0
OP1 G:DG34 4.9 37.5 1.0
CA A:LYS102 5.0 17.9 1.0
CG A:GLU55 5.0 23.5 1.0

Calcium binding site 2 out of 2 in 2ezv

Go back to Calcium Binding Sites List in 2ezv
Calcium binding site 2 out of 2 in the Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Tetrameric Restriction Endonuclease Sfii Bound to Cognate Dna. within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca270

b:61.3
occ:1.00
OD1 B:ASP100 2.6 32.1 1.0
OD2 B:ASP79 2.6 28.5 1.0
O B:ALA101 2.8 22.0 1.0
CG B:ASP100 3.2 31.4 1.0
OD2 B:ASP100 3.2 33.0 1.0
CG B:ASP79 3.5 27.4 1.0
OD1 B:ASP79 3.6 30.2 1.0
C B:ALA101 3.9 22.4 1.0
OP1 F:DA13 4.0 66.3 1.0
N B:ALA101 4.0 19.6 1.0
OE2 B:GLU55 4.2 28.0 1.0
NZ B:LYS102 4.2 32.0 1.0
NE2 B:GLN116 4.3 31.1 1.0
CD B:LYS102 4.3 29.9 1.0
O B:HOH292 4.4 28.4 1.0
CB B:ASP100 4.5 25.5 1.0
CE B:LYS102 4.5 31.0 1.0
OP2 F:DA13 4.5 66.0 1.0
CA B:ALA101 4.5 21.0 1.0
P F:DA13 4.6 66.7 1.0
C B:ASP100 4.7 21.4 1.0
O5' F:DA13 4.8 64.6 1.0
CA B:ASP100 4.8 22.5 1.0
CB B:ASP79 4.9 24.5 1.0
N B:LYS102 5.0 23.8 1.0

Reference:

E.S.Vanamee, H.Viadiu, R.Kucera, L.Dorner, S.Picone, I.Schildkraut, A.K.Aggarwal. A View of Consecutive Binding Events From Structures of Tetrameric Endonuclease Sfii Bound to Dna Embo J. V. 24 4198 2005.
ISSN: ISSN 0261-4189
PubMed: 16308566
DOI: 10.1038/SJ.EMBOJ.7600880
Page generated: Fri Jul 12 10:20:07 2024

Last articles

Mg in 4Y52
Mg in 4Y30
Mg in 4Y2V
Mg in 4Y2X
Mg in 4Y2Y
Mg in 4Y2U
Mg in 4Y2T
Mg in 4Y2Q
Mg in 4Y2R
Mg in 4Y2S
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy