Calcium in PDB 2fps: Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model.

All present enzymatic activity of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model.:
3.1.3.15;

The structure of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model., PDB code: 2fps was solved by E.S.Rangarajan, M.Cygler, A.Matte, Montreal-Kingston Bacterialstructural Genomics Initiative (Bsgi), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.20
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	52.748, 131.325, 104.898, 90.00, 90.00, 90.00
R / Rfree (%)	17 / 24.4

The structure of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model. also contains other interesting chemical elements:

Chlorine	(Cl)	2 atoms
Zinc	(Zn)	2 atoms

The binding sites of Calcium atom in the Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model. (pdb code 2fps). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model., PDB code: 2fps:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model. within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca503 b:17.7 occ:1.00 O A:ASP12 2.3 12.1 1.0 OD1 A:ASP131 2.3 15.2 1.0 O A:HOH529 2.3 16.1 1.0 O A:HOH509 2.3 13.3 1.0 OD2 A:ASP10 2.4 12.4 1.0 O A:HOH528 2.4 10.6 1.0 CG A:ASP10 3.2 14.2 1.0 CG A:ASP131 3.3 15.4 1.0 OD1 A:ASP10 3.3 14.7 1.0 C A:ASP12 3.5 12.4 1.0 OD2 A:ASP131 3.6 15.1 1.0 O A:HOH508 4.1 13.9 1.0 CD A:ARG132 4.2 18.8 1.0 NE A:ARG132 4.3 22.2 1.0 OG1 A:THR14 4.3 11.7 1.0 CG A:ARG132 4.3 16.6 1.0 OD2 A:ASP135 4.4 14.7 1.0 N A:GLY13 4.4 11.9 1.0 CA A:GLY13 4.4 12.1 1.0 CA A:ASP12 4.4 13.3 1.0 OE2 A:GLU18 4.5 21.0 1.0 CL A:CL505 4.5 27.1 1.0 O A:HOH559 4.5 26.3 1.0 CB A:ASP10 4.5 13.6 1.0 N A:ASP12 4.5 13.4 1.0 CB A:ASP12 4.6 13.7 1.0 CB A:ASP131 4.6 15.3 1.0 N A:ASP131 4.7 14.9 1.0 C A:GLY13 4.8 12.0 1.0 N A:ARG132 5.0 15.8 1.0 CB A:ARG132 5.0 16.9 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model. within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca504 b:18.7 occ:1.00 OD1 B:ASP131 2.3 15.7 1.0 O B:ASP12 2.3 13.7 1.0 OD2 B:ASP10 2.3 17.6 1.0 O B:HOH515 2.3 13.3 1.0 O B:HOH527 2.3 19.8 1.0 O B:HOH539 2.5 14.1 1.0 CG B:ASP10 3.2 14.4 1.0 CG B:ASP131 3.3 15.3 1.0 OD1 B:ASP10 3.4 15.4 1.0 C B:ASP12 3.5 14.0 1.0 OD2 B:ASP131 3.6 16.9 1.0 O B:HOH528 4.2 15.1 1.0 CG B:ARG132 4.2 19.5 1.0 OD2 B:ASP135 4.3 16.8 1.0 OG1 B:THR14 4.3 12.4 1.0 NE B:ARG132 4.4 29.2 1.0 N B:GLY13 4.4 13.0 1.0 CA B:ASP12 4.4 14.7 1.0 O B:HOH678 4.4 17.6 1.0 CA B:GLY13 4.4 12.8 1.0 N B:ASP12 4.5 14.3 1.0 CL B:CL506 4.5 28.5 1.0 CB B:ASP10 4.5 14.0 1.0 CB B:ASP12 4.6 14.6 1.0 CD B:ARG132 4.6 26.3 1.0 CB B:ASP131 4.7 15.0 1.0 N B:ASP131 4.7 14.9 1.0 OE2 B:GLU18 4.7 15.1 1.0 C B:GLY13 4.8 12.4 1.0 O B:HOH541 4.9 17.1 1.0 N B:THR14 5.0 12.0 1.0

E.S.Rangarajan, A.Proteau, J.Wagner, M.N.Hung, A.Matte, M.Cygler. Structural Snapshots of Escherichia Coli Histidinol Phosphate Phosphatase Along the Reaction Pathway. J.Biol.Chem. V. 281 37930 2006.
ISSN: ISSN 0021-9258
PubMed: 16966333
DOI: 10.1074/JBC.M604916200