Calcium in PDB 2g8s: Crystal Structure of the Soluble Aldose Sugar Dehydrogenase (Asd) From Escherichia Coli in the Apo-Form

The structure of Crystal Structure of the Soluble Aldose Sugar Dehydrogenase (Asd) From Escherichia Coli in the Apo-Form, PDB code: 2g8s was solved by S.M.Southall, J.J.Doel, D.J.Richardson, A.Oubrie, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	7.96 / 1.50
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	40.829, 113.475, 75.372, 90.00, 90.03, 90.00
R / Rfree (%)	14.9 / 18.3

The binding sites of Calcium atom in the Crystal Structure of the Soluble Aldose Sugar Dehydrogenase (Asd) From Escherichia Coli in the Apo-Form (pdb code 2g8s). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of the Soluble Aldose Sugar Dehydrogenase (Asd) From Escherichia Coli in the Apo-Form, PDB code: 2g8s:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of the Soluble Aldose Sugar Dehydrogenase (Asd) From Escherichia Coli in the Apo-Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the Soluble Aldose Sugar Dehydrogenase (Asd) From Escherichia Coli in the Apo-Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca2002 b:5.4 occ:1.00 O A:HOH3060 2.4 6.8 1.0 O A:HOH3037 2.4 7.6 1.0 O A:HOH3027 2.4 6.0 1.0 O A:TYR230 2.4 6.5 1.0 O A:HOH3029 2.5 5.8 1.0 OE1 A:GLU220 2.5 5.6 1.0 OE2 A:GLU220 2.5 6.0 1.0 CD A:GLU220 2.9 5.2 1.0 C A:TYR230 3.6 6.4 1.0 O1 A:EDO3002 4.0 9.9 1.0 O A:HOH3036 4.3 10.0 1.0 CB A:TYR230 4.3 5.8 1.0 CG A:GLU220 4.4 5.8 1.0 CA A:TYR230 4.4 6.0 1.0 ND2 A:ASN222 4.5 6.7 1.0 O A:GLU258 4.5 7.2 1.0 O A:GLY192 4.5 6.0 1.0 OE2 A:GLU212 4.6 9.2 1.0 N A:GLY231 4.6 6.4 1.0 OD1 A:ASN222 4.6 6.6 1.0 O A:ALA235 4.6 6.2 1.0 CD A:PRO260 4.7 6.0 1.0 N A:TRP232 4.7 5.5 1.0 O A:HOH3028 4.7 6.3 1.0 O2 A:EDO3002 4.7 7.8 1.0 C1 A:EDO3002 4.7 10.1 1.0 O A:HOH3035 4.7 7.5 1.0 CA A:GLY231 4.8 6.6 1.0 CD A:GLU212 5.0 8.5 1.0 CG A:ASN222 5.0 5.7 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of the Soluble Aldose Sugar Dehydrogenase (Asd) From Escherichia Coli in the Apo-Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the Soluble Aldose Sugar Dehydrogenase (Asd) From Escherichia Coli in the Apo-Form within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca2001 b:5.3 occ:1.00 O B:HOH3038 2.3 7.0 1.0 O B:TYR230 2.4 6.2 1.0 O B:HOH3023 2.4 5.8 1.0 O B:HOH3043 2.4 7.5 1.0 O B:HOH3024 2.4 6.0 1.0 OE1 B:GLU220 2.5 6.1 1.0 OE2 B:GLU220 2.5 6.4 1.0 CD B:GLU220 2.8 5.0 1.0 C B:TYR230 3.6 6.2 1.0 O2 B:EDO3003 4.1 10.0 1.0 O B:HOH3039 4.3 9.7 1.0 CB B:TYR230 4.3 6.0 1.0 CG B:GLU220 4.4 5.5 1.0 CA B:TYR230 4.4 6.2 1.0 O B:GLU258 4.5 6.5 1.0 O B:GLY192 4.5 6.1 1.0 OD1 B:ASN222 4.5 8.8 1.0 OE2 B:GLU212 4.6 10.1 1.0 N B:GLY231 4.6 6.1 1.0 ND2 B:ASN222 4.6 4.6 1.0 O B:ALA235 4.6 7.1 1.0 CD B:PRO260 4.7 5.9 1.0 N B:TRP232 4.7 5.8 1.0 O1 B:EDO3003 4.7 7.3 1.0 O B:HOH3026 4.7 6.6 1.0 O B:HOH3028 4.7 7.0 1.0 CA B:GLY231 4.7 6.0 1.0 C2 B:EDO3003 4.8 9.6 1.0 CD B:GLU212 5.0 9.8 1.0

S.M.Southall, J.J.Doel, D.J.Richardson, A.Oubrie. Soluble Aldose Sugar Dehydrogenase From Escherichia Coli: A Highly Exposed Active Site Conferring Broad Substrate Specificity. J.Biol.Chem. V. 281 30650 2006.
ISSN: ISSN 0021-9258
PubMed: 16864586
DOI: 10.1074/JBC.M601783200