Calcium in PDB 2gij: Q138F Hincii Bound to Cognate Dna Gttaac and CA2+

All present enzymatic activity of Q138F Hincii Bound to Cognate Dna Gttaac and CA2+:
3.1.21.4;

The structure of Q138F Hincii Bound to Cognate Dna Gttaac and CA2+, PDB code: 2gij was solved by N.C.Horton, H.K.Joshi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.93
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	50.462, 91.773, 66.543, 90.00, 104.46, 90.00
R / Rfree (%)	23.3 / 26.3

The structure of Q138F Hincii Bound to Cognate Dna Gttaac and CA2+ also contains other interesting chemical elements:

Sodium

(Na)

1 atom

The binding sites of Calcium atom in the Q138F Hincii Bound to Cognate Dna Gttaac and CA2+ (pdb code 2gij). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Q138F Hincii Bound to Cognate Dna Gttaac and CA2+, PDB code: 2gij:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Q138F Hincii Bound to Cognate Dna Gttaac and CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Q138F Hincii Bound to Cognate Dna Gttaac and CA2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:36.5 occ:1.00 OP1 F:DA8 2.3 36.8 1.0 OD1 A:ASP114 2.3 28.6 1.0 O A:VAL128 2.3 33.7 1.0 O A:HOH412 2.3 41.0 1.0 OD2 A:ASP127 2.4 33.7 1.0 OP2 F:DA9 2.5 38.1 1.0 CG A:ASP114 3.3 29.9 1.0 C A:VAL128 3.4 36.7 1.0 P F:DA8 3.5 34.3 1.0 OD2 A:ASP114 3.6 30.9 1.0 N A:VAL128 3.6 36.5 1.0 CG A:ASP127 3.6 38.5 1.0 P F:DA9 3.9 43.0 1.0 O5' F:DA8 4.0 34.1 1.0 CA A:VAL128 4.0 33.9 1.0 OP2 F:DA8 4.1 36.8 1.0 NZ A:LYS129 4.2 40.0 1.0 C A:ASP127 4.2 35.5 1.0 O A:HOH484 4.3 42.0 1.0 C5' F:DA8 4.3 34.0 1.0 OD1 A:ASP127 4.3 39.4 1.0 CE A:LYS129 4.3 40.6 1.0 OP1 F:DA9 4.4 41.8 1.0 CB A:VAL128 4.4 36.9 1.0 O3' F:DA8 4.5 39.5 1.0 N A:LYS129 4.5 37.2 1.0 CA A:ASP127 4.5 35.5 1.0 CB A:ASP114 4.6 28.9 1.0 CD A:LYS129 4.7 40.9 1.0 CB A:ASP127 4.7 37.8 1.0 C3' F:DA8 4.8 36.5 1.0 O3' F:DT7 4.8 33.5 1.0 CA A:LYS129 4.8 39.2 1.0

Calcium binding site 2 out of 2 in the Q138F Hincii Bound to Cognate Dna Gttaac and CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Q138F Hincii Bound to Cognate Dna Gttaac and CA2+ within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca402 b:52.0 occ:1.00 O B:HOH465 2.2 29.9 1.0 OP1 E:DA8 2.4 56.0 1.0 OD2 B:ASP114 2.5 45.5 1.0 O B:HOH454 3.0 36.9 1.0 OD1 B:ASP127 3.3 54.9 1.0 CG B:ASP114 3.3 45.6 1.0 O B:VAL128 3.5 50.9 1.0 OD1 B:ASP114 3.5 48.2 1.0 P E:DA8 3.5 53.6 1.0 O E:HOH29 3.5 37.9 1.0 O B:HOH408 3.6 50.6 1.0 O B:HOH456 3.7 46.3 1.0 O E:HOH44 3.7 36.6 1.0 CG B:ASP127 3.8 53.9 1.0 O5' E:DA8 3.9 55.5 1.0 OP2 E:DA8 4.1 54.1 1.0 OP2 E:DA9 4.2 65.7 1.0 OD2 B:ASP127 4.3 53.7 1.0 CB B:ASP127 4.4 51.9 1.0 N B:VAL128 4.4 51.0 1.0 C B:VAL128 4.5 51.6 1.0 O E:HOH66 4.5 47.2 1.0 O E:HOH54 4.6 43.5 1.0 CB B:ASP114 4.7 41.6 1.0 CA B:ASP127 4.8 49.9 1.0 O3' E:DT7 4.8 51.8 1.0 C3' E:DA8 4.9 62.9 1.0 O B:HOH473 4.9 59.6 1.0 C B:ASP127 4.9 50.3 1.0

H.K.Joshi, C.Etzkorn, L.Chatwell, J.Bitinaite, N.C.Horton. Alteration of Sequence Specificity of the Type II Restriction Endonuclease Hincii Through An Indirect Readout Mechanism. J.Biol.Chem. V. 281 23852 2006.
ISSN: ISSN 0021-9258
PubMed: 16675462
DOI: 10.1074/JBC.M512339200