Calcium in PDB 2gsp: Ribonuclease T1/2',3'-Cgps and 3'-Gmp, 2 Days

All present enzymatic activity of Ribonuclease T1/2',3'-Cgps and 3'-Gmp, 2 Days:
3.1.27.3;

The structure of Ribonuclease T1/2',3'-Cgps and 3'-Gmp, 2 Days, PDB code: 2gsp was solved by I.Zegers, L.Wyns, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	48.120, 51.090, 40.560, 90.00, 90.00, 90.00
R / Rfree (%)	15.9 / 19.7

The binding sites of Calcium atom in the Ribonuclease T1/2',3'-Cgps and 3'-Gmp, 2 Days (pdb code 2gsp). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Ribonuclease T1/2',3'-Cgps and 3'-Gmp, 2 Days, PDB code: 2gsp:

Calcium binding site 1 out of 1 in the Ribonuclease T1/2',3'-Cgps and 3'-Gmp, 2 Days


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ribonuclease T1/2',3'-Cgps and 3'-Gmp, 2 Days within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca107 b:37.7 occ:1.00 O A:HOH141 2.2 33.9 1.0 OD2 A:ASP15 2.5 12.5 1.0 O A:HOH149 2.5 32.7 1.0 O A:HOH122 2.5 24.7 1.0 OD1 A:ASP15 2.6 13.3 1.0 O A:HOH127 2.7 23.4 1.0 CG A:ASP15 2.8 13.8 1.0 O A:HOH168 3.0 43.7 1.0 O A:HOH187 3.4 47.9 1.0 CB A:ASP15 4.3 11.2 1.0 OG A:SER12 4.5 13.2 1.0 O A:CYS10 4.6 12.2 1.0 O A:HOH157 4.7 36.3 1.0 O A:HOH117 4.8 17.1 1.0 O A:HOH144 4.9 34.1 1.0 N A:SER12 4.9 12.0 1.0

I.Zegers, R.Loris, G.Dehollander, A.Fattah Haikal, F.Poortmans, J.Steyaert, L.Wyns. Hydrolysis of A Slow Cyclic Thiophosphate Substrate of Rnase T1 Analyzed By Time-Resolved Crystallography. Nat.Struct.Biol. V. 5 280 1998.
ISSN: ISSN 1072-8368
PubMed: 9546218
DOI: 10.1038/NSB0498-280