Calcium in PDB 2h0l: Crystal Structure of A Mutant of Rat Annexin A5

The structure of Crystal Structure of A Mutant of Rat Annexin A5, PDB code: 2h0l was solved by B.Langlois D'estaintot, B.Gallois, T.Granier, B.Tessier, A.Brisson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.76 / 2.59
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	77.918, 78.959, 60.458, 90.00, 90.00, 90.00
R / Rfree (%)	18.7 / 27.8

The binding sites of Calcium atom in the Crystal Structure of A Mutant of Rat Annexin A5 (pdb code 2h0l). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Crystal Structure of A Mutant of Rat Annexin A5, PDB code: 2h0l:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Crystal Structure of A Mutant of Rat Annexin A5


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of A Mutant of Rat Annexin A5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1200 b:35.4 occ:0.50 O A:MET26 2.3 39.0 1.0 O A:GLY30 2.3 32.7 1.0 O A:GLY28 2.3 43.9 1.0 OE1 A:GLU70 2.7 47.0 1.0 OE2 A:GLU70 2.8 45.3 1.0 CD A:GLU70 3.1 46.5 1.0 C A:MET26 3.4 44.3 1.0 C A:GLY28 3.4 47.4 1.0 C A:GLY30 3.5 41.8 1.0 OG1 A:THR31 3.8 27.9 1.0 N A:GLY30 3.9 47.8 1.0 C A:LEU29 3.9 51.2 1.0 CA A:MET26 3.9 40.0 1.0 CA A:LEU29 4.2 50.7 1.0 N A:LEU29 4.2 50.9 1.0 N A:GLY28 4.3 52.3 1.0 O A:LEU29 4.3 59.3 1.0 CA A:GLY30 4.3 45.3 1.0 C A:GLU27 4.3 54.3 1.0 N A:THR31 4.4 35.7 1.0 CA A:GLY28 4.4 48.7 1.0 N A:GLU27 4.5 52.6 1.0 CA A:THR31 4.5 27.6 1.0 CG A:GLU70 4.6 45.9 1.0 O A:GLU27 4.6 50.2 1.0 CB A:MET26 4.6 37.2 1.0 CB A:THR31 4.7 23.9 1.0 CA A:GLU27 4.8 56.0 1.0

Calcium binding site 2 out of 3 in the Crystal Structure of A Mutant of Rat Annexin A5


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of A Mutant of Rat Annexin A5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1202 b:40.0 occ:0.50 O A:GLY261 2.3 43.6 1.0 O A:MET257 2.3 36.4 1.0 O A:GLY259 2.4 59.5 1.0 OD2 A:ASP301 2.8 51.1 1.0 C A:GLY259 3.3 63.3 1.0 C A:MET257 3.4 39.1 1.0 C A:GLY261 3.4 47.3 1.0 OD1 A:ASP301 3.5 53.1 1.0 CG A:ASP301 3.5 51.1 1.0 O A:ALA260 3.6 65.6 1.0 C A:ALA260 3.7 60.9 1.0 N A:GLY259 3.8 54.5 1.0 N A:GLY261 3.8 52.1 1.0 C A:LYS258 3.9 54.1 1.0 CA A:GLY261 4.0 50.1 1.0 CA A:GLY259 4.1 61.2 1.0 O A:LYS258 4.2 62.8 1.0 N A:ALA260 4.2 64.2 1.0 CA A:MET257 4.2 37.8 1.0 N A:LYS258 4.4 39.6 1.0 OG1 A:THR262 4.4 30.1 1.0 CA A:ALA260 4.5 62.5 1.0 N A:THR262 4.5 37.2 1.0 CA A:LYS258 4.5 47.7 1.0 CA A:THR262 4.8 33.6 1.0 CB A:MET257 4.9 35.9 1.0 CB A:ASP301 5.0 48.3 1.0

Calcium binding site 3 out of 3 in the Crystal Structure of A Mutant of Rat Annexin A5


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of A Mutant of Rat Annexin A5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1015 b:26.3 occ:1.00 ND1 A:HIS96 2.2 31.8 1.0 NE2 A:HIS265 2.2 42.5 1.0 O A:HOH322 2.3 31.8 1.0 OE2 A:GLU93 2.3 32.3 1.0 O A:HOH321 2.4 27.4 1.0 CD A:GLU93 2.8 30.3 1.0 OE1 A:GLU93 2.8 31.4 1.0 CE1 A:HIS265 2.9 44.9 1.0 CE1 A:HIS96 3.1 33.1 1.0 CG A:HIS96 3.3 34.1 1.0 CD2 A:HIS265 3.4 44.9 1.0 CB A:HIS96 3.6 34.6 1.0 ND1 A:HIS265 4.1 45.0 1.0 CG A:GLU93 4.2 31.4 1.0 NE2 A:HIS96 4.3 35.9 1.0 CG A:HIS265 4.3 40.2 1.0 CA A:GLU93 4.3 24.5 1.0 CD2 A:HIS96 4.3 34.9 1.0 O A:GLU93 4.4 30.3 1.0 CB A:GLU93 4.5 30.4 1.0 CE2 A:TYR92 4.5 33.6 1.0 NH1 A:ARG269 4.6 33.9 1.0 O A:HOH384 4.8 39.2 1.0 C A:GLU93 4.9 27.8 1.0 CA A:HIS96 5.0 33.5 1.0

A.Brisson, T.Granier, B.Langlois D'estaintot, B.Gallois, B.Tessier. Identification of the Residues Involved in the Formation of Annexin V Trimers Within 2D and 3D Crystals To Be Published.