Calcium in PDB 2het: Non-Myristoylated Bovine Recoverin (Truncated at C-Terminus) with Calcium Bound to Ef-Hand 3

The structure of Non-Myristoylated Bovine Recoverin (Truncated at C-Terminus) with Calcium Bound to Ef-Hand 3, PDB code: 2het was solved by O.H.Weiergraber, J.Granzin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	70.00 / 3.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	70.889, 70.759, 85.925, 90.00, 99.01, 90.00
R / Rfree (%)	23.7 / 25.9

The binding sites of Calcium atom in the Non-Myristoylated Bovine Recoverin (Truncated at C-Terminus) with Calcium Bound to Ef-Hand 3 (pdb code 2het). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Non-Myristoylated Bovine Recoverin (Truncated at C-Terminus) with Calcium Bound to Ef-Hand 3, PDB code: 2het:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Non-Myristoylated Bovine Recoverin (Truncated at C-Terminus) with Calcium Bound to Ef-Hand 3


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Non-Myristoylated Bovine Recoverin (Truncated at C-Terminus) with Calcium Bound to Ef-Hand 3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca501 b:49.5 occ:1.00 OD1 A:ASP110 2.3 52.0 1.0 OD1 A:ASN114 2.4 66.4 1.0 OE1 A:GLU121 2.4 63.6 1.0 OD1 A:ASP112 2.4 65.9 1.0 O A:THR116 2.5 67.8 1.0 CD A:GLU121 3.1 63.6 1.0 OE2 A:GLU121 3.2 63.6 1.0 CG A:ASN114 3.2 66.4 1.0 ND2 A:ASN114 3.4 66.4 1.0 CG A:ASP110 3.6 52.0 1.0 CG A:ASP112 3.6 65.9 1.0 C A:THR116 3.7 67.8 1.0 CA A:ASP110 4.1 53.3 1.0 N A:ASP112 4.1 54.6 1.0 CB A:ASP112 4.2 65.9 1.0 C A:ASP110 4.2 53.3 1.0 CB A:ASP110 4.3 52.0 1.0 N A:ASN114 4.4 81.0 1.0 N A:GLY113 4.4 66.3 1.0 OG A:SER118 4.4 67.5 1.0 CA A:ILE117 4.4 46.5 1.0 N A:VAL111 4.4 46.5 1.0 CA A:ASP112 4.5 54.6 1.0 OD2 A:ASP110 4.5 52.0 1.0 N A:THR116 4.5 67.8 1.0 N A:ILE117 4.5 46.5 1.0 C A:ASP112 4.6 54.6 1.0 CG A:GLU121 4.6 63.6 1.0 OG1 A:THR116 4.6 79.3 1.0 OD2 A:ASP112 4.6 65.9 1.0 CB A:ASN114 4.6 66.4 1.0 CA A:THR116 4.7 67.8 1.0 O A:ASP110 4.7 53.3 1.0 N A:SER118 4.9 60.6 1.0 N A:GLY115 5.0 69.5 1.0

Calcium binding site 2 out of 4 in the Non-Myristoylated Bovine Recoverin (Truncated at C-Terminus) with Calcium Bound to Ef-Hand 3


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Non-Myristoylated Bovine Recoverin (Truncated at C-Terminus) with Calcium Bound to Ef-Hand 3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca502 b:49.5 occ:1.00 OD1 B:ASP110 2.3 52.0 1.0 OD1 B:ASN114 2.4 66.4 1.0 OE1 B:GLU121 2.4 63.6 1.0 OD1 B:ASP112 2.4 65.9 1.0 O B:THR116 2.5 67.8 1.0 CD B:GLU121 3.1 63.6 1.0 OE2 B:GLU121 3.2 63.6 1.0 CG B:ASN114 3.2 66.4 1.0 ND2 B:ASN114 3.4 66.4 1.0 CG B:ASP110 3.6 52.0 1.0 CG B:ASP112 3.6 65.9 1.0 C B:THR116 3.7 67.8 1.0 CA B:ASP110 4.1 53.3 1.0 N B:ASP112 4.1 54.6 1.0 CB B:ASP112 4.2 65.9 1.0 C B:ASP110 4.2 53.3 1.0 CB B:ASP110 4.3 52.0 1.0 N B:ASN114 4.4 81.0 1.0 N B:GLY113 4.4 66.3 1.0 OG B:SER118 4.4 67.5 1.0 CA B:ILE117 4.4 46.5 1.0 N B:VAL111 4.5 46.5 1.0 CA B:ASP112 4.5 54.6 1.0 N B:THR116 4.5 67.8 1.0 OD2 B:ASP110 4.5 52.0 1.0 N B:ILE117 4.5 46.5 1.0 C B:ASP112 4.6 54.6 1.0 CG B:GLU121 4.6 63.6 1.0 OG1 B:THR116 4.6 79.3 1.0 OD2 B:ASP112 4.6 65.9 1.0 CB B:ASN114 4.6 66.4 1.0 CA B:THR116 4.7 67.8 1.0 O B:ASP110 4.8 53.3 1.0 N B:SER118 4.9 60.6 1.0 N B:GLY115 5.0 69.5 1.0

Calcium binding site 3 out of 4 in the Non-Myristoylated Bovine Recoverin (Truncated at C-Terminus) with Calcium Bound to Ef-Hand 3


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Non-Myristoylated Bovine Recoverin (Truncated at C-Terminus) with Calcium Bound to Ef-Hand 3 within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca503 b:49.5 occ:1.00 OD1 C:ASP110 2.3 52.0 1.0 OD1 C:ASN114 2.4 66.4 1.0 OE1 C:GLU121 2.4 63.6 1.0 OD1 C:ASP112 2.4 65.9 1.0 O C:THR116 2.5 67.8 1.0 CD C:GLU121 3.1 63.6 1.0 OE2 C:GLU121 3.2 63.6 1.0 CG C:ASN114 3.2 66.4 1.0 ND2 C:ASN114 3.4 66.4 1.0 CG C:ASP110 3.6 52.0 1.0 CG C:ASP112 3.6 65.9 1.0 C C:THR116 3.7 67.8 1.0 CA C:ASP110 4.1 53.3 1.0 N C:ASP112 4.1 54.6 1.0 CB C:ASP112 4.2 65.9 1.0 C C:ASP110 4.2 53.3 1.0 CB C:ASP110 4.3 52.0 1.0 N C:ASN114 4.4 81.0 1.0 N C:GLY113 4.4 66.3 1.0 OG C:SER118 4.4 67.5 1.0 CA C:ILE117 4.4 46.5 1.0 N C:VAL111 4.5 46.5 1.0 CA C:ASP112 4.5 54.6 1.0 OD2 C:ASP110 4.5 52.0 1.0 N C:THR116 4.5 67.8 1.0 N C:ILE117 4.5 46.5 1.0 C C:ASP112 4.6 54.6 1.0 CG C:GLU121 4.6 63.6 1.0 OG1 C:THR116 4.6 79.3 1.0 OD2 C:ASP112 4.6 65.9 1.0 CB C:ASN114 4.6 66.4 1.0 CA C:THR116 4.7 67.8 1.0 O C:ASP110 4.8 53.3 1.0 N C:SER118 4.9 60.6 1.0 N C:GLY115 5.0 69.5 1.0

Calcium binding site 4 out of 4 in the Non-Myristoylated Bovine Recoverin (Truncated at C-Terminus) with Calcium Bound to Ef-Hand 3


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Non-Myristoylated Bovine Recoverin (Truncated at C-Terminus) with Calcium Bound to Ef-Hand 3 within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca504 b:49.5 occ:1.00 OD1 D:ASP110 2.3 52.0 1.0 OD1 D:ASN114 2.4 66.4 1.0 OE1 D:GLU121 2.4 63.6 1.0 OD1 D:ASP112 2.4 65.9 1.0 O D:THR116 2.5 67.8 1.0 CD D:GLU121 3.1 63.6 1.0 OE2 D:GLU121 3.2 63.6 1.0 CG D:ASN114 3.2 66.4 1.0 ND2 D:ASN114 3.4 66.4 1.0 CG D:ASP110 3.6 52.0 1.0 CG D:ASP112 3.6 65.9 1.0 C D:THR116 3.7 67.8 1.0 CA D:ASP110 4.1 53.3 1.0 N D:ASP112 4.1 54.6 1.0 CB D:ASP112 4.2 65.9 1.0 C D:ASP110 4.2 53.3 1.0 CB D:ASP110 4.3 52.0 1.0 N D:ASN114 4.4 81.0 1.0 N D:GLY113 4.4 66.3 1.0 OG D:SER118 4.4 67.5 1.0 CA D:ILE117 4.4 46.5 1.0 N D:VAL111 4.4 46.5 1.0 CA D:ASP112 4.5 54.6 1.0 OD2 D:ASP110 4.5 52.0 1.0 N D:THR116 4.5 67.8 1.0 N D:ILE117 4.5 46.5 1.0 C D:ASP112 4.6 54.6 1.0 CG D:GLU121 4.6 63.6 1.0 OG1 D:THR116 4.6 79.3 1.0 OD2 D:ASP112 4.6 65.9 1.0 CB D:ASN114 4.6 66.4 1.0 CA D:THR116 4.7 67.8 1.0 O D:ASP110 4.7 53.3 1.0 N D:SER118 4.9 60.6 1.0 N D:GLY115 5.0 69.5 1.0

O.H.Weiergraber, I.I.Senin, E.Y.Zernii, V.A.Churumova, N.A.Kovaleva, A.A.Nazipova, S.E.Permyakov, E.A.Permyakov, P.P.Philippov, J.Granzin, K.W.Koch. Tuning of A Neuronal Calcium Sensor. J.Biol.Chem. V. 281 37594 2006.
ISSN: ISSN 0021-9258
PubMed: 17015448
DOI: 10.1074/JBC.M603700200