Calcium in PDB 2hf3: Crystal Structure of Monomeric Actin in the Adp Bound State

The structure of Crystal Structure of Monomeric Actin in the Adp Bound State, PDB code: 2hf3 was solved by M.A.Rould, Q.Wan, P.B.Joel, S.Lowey, K.M.Trybus, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.19 / 1.80
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	199.700, 54.070, 39.590, 90.00, 93.16, 90.00
R / Rfree (%)	17.5 / 21.3

The binding sites of Calcium atom in the Crystal Structure of Monomeric Actin in the Adp Bound State (pdb code 2hf3). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Monomeric Actin in the Adp Bound State, PDB code: 2hf3:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Monomeric Actin in the Adp Bound State


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Monomeric Actin in the Adp Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca901 b:12.4 occ:1.00 O1B A:ADP800 2.3 8.8 1.0 O A:HOH1004 2.3 9.0 1.0 O A:HOH1003 2.3 7.7 1.0 O A:HOH1011 2.4 10.2 1.0 O A:HOH1040 2.4 14.6 1.0 O A:HOH1002 2.5 7.2 1.0 O A:HOH1043 2.9 15.2 1.0 PB A:ADP800 3.5 9.9 1.0 O3B A:ADP800 3.7 12.0 1.0 OE1 A:GLN137 4.1 8.5 1.0 CA A:GLY13 4.2 10.9 1.0 O3A A:ADP800 4.3 9.1 1.0 O1A A:ADP800 4.3 10.8 1.0 O A:HOH1033 4.4 14.7 1.0 NZ A:LYS18 4.4 9.9 1.0 CD A:GLN137 4.4 9.1 1.0 O A:HOH1009 4.5 10.7 1.0 OD1 A:ASP154 4.6 10.4 1.0 OD2 A:ASP11 4.6 9.7 1.0 OD1 A:ASP11 4.6 9.1 1.0 OD2 A:ASP154 4.6 11.5 1.0 O A:HOH1082 4.6 20.2 1.0 O2B A:ADP800 4.7 11.6 1.0 PA A:ADP800 4.8 10.0 1.0 O A:ASN12 4.9 11.6 1.0 O A:HOH1062 4.9 18.1 1.0 CG A:GLN137 4.9 8.5 1.0 N A:SER14 4.9 13.3 1.0 NE2 A:GLN137 5.0 7.6 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Monomeric Actin in the Adp Bound State


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Monomeric Actin in the Adp Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca902 b:21.5 occ:1.00 O A:HOH1050 2.2 16.3 1.0 O A:HOH1171 2.3 27.0 1.0 OG A:SER265 2.4 12.3 1.0 OE1 A:GLN263 2.4 11.7 1.0 O A:HOH1220 2.5 28.6 1.0 CD A:GLN263 3.4 10.3 1.0 CB A:SER265 3.4 11.3 1.0 NE2 A:GLN263 3.7 13.1 1.0 N A:SER265 4.0 11.2 1.0 CA A:SER265 4.2 12.0 1.0 O A:HOH1199 4.4 28.3 1.0 O A:MET269 4.4 18.3 1.0 O A:ALA271 4.6 14.7 1.0 O A:HOH1166 4.6 27.2 1.0 CG A:GLN263 4.8 10.9 1.0 O A:GLU270 4.8 17.2 1.0 CD A:PRO264 4.9 9.1 1.0 N A:PRO264 4.9 9.4 1.0 O A:HOH1490 5.0 44.5 1.0

M.A.Rould, Q.Wan, P.B.Joel, S.Lowey, K.M.Trybus. Crystal Structures of Expressed Non-Polymerizable Monomeric Actin in the Adp and Atp States. J.Biol.Chem. V. 281 31909 2006.
ISSN: ISSN 0021-9258
PubMed: 16920713
DOI: 10.1074/JBC.M601973200