Calcium in PDB 2ie7: Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide

The structure of Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide, PDB code: 2ie7 was solved by N.Colloc'h, J.Sopkova-De Oliveira Santos, T.Prange, A.Brisson, B.Langloisd'estainto, B.Gallois, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.84 / 1.75
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	157.028, 157.028, 37.327, 90.00, 90.00, 120.00
R / Rfree (%)	17.4 / 20.3

The binding sites of Calcium atom in the Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide (pdb code 2ie7). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 7 binding sites of Calcium where determined in the Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide, PDB code: 2ie7:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6; 7;

Calcium binding site 1 out of 7 in the Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:16.4 occ:1.00 O A:GLY181 2.3 14.7 1.0 O A:LYS184 2.3 17.5 1.0 O A:HOH559 2.4 18.1 1.0 O4 A:SO4420 2.4 35.0 0.7 O A:GLY186 2.4 19.0 1.0 OE1 A:GLU226 2.5 17.8 1.0 OE2 A:GLU226 2.5 21.6 1.0 O1 A:SO4420 2.6 37.7 0.7 CD A:GLU226 2.8 18.6 1.0 S A:SO4420 3.0 35.4 0.7 C A:GLY181 3.4 16.5 1.0 C A:LYS184 3.5 16.2 1.0 C A:GLY186 3.6 18.7 1.0 N A:GLY186 3.8 16.9 1.0 O3 A:SO4420 4.1 37.6 0.7 O2 A:SO4420 4.1 37.0 0.7 N A:LYS184 4.2 16.7 1.0 CA A:GLY181 4.2 15.9 1.0 CA A:GLY186 4.2 18.0 1.0 CA A:LYS184 4.3 16.7 1.0 OG1 A:THR187 4.3 20.0 1.0 N A:GLU182 4.3 14.8 1.0 CG A:GLU226 4.4 18.7 1.0 CB A:LYS184 4.4 17.5 1.0 CA A:GLU182 4.4 14.5 1.0 C A:GLU182 4.5 16.3 1.0 O A:HOH593 4.5 38.8 1.0 O A:HOH525 4.5 26.6 1.0 O A:GLU182 4.5 17.5 1.0 N A:TRP185 4.5 16.3 1.0 C A:TRP185 4.6 16.4 1.0 N A:THR187 4.6 18.2 1.0 CA A:TRP185 4.6 16.3 1.0 CA A:THR187 4.7 18.6 1.0

Calcium binding site 2 out of 7 in the Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:32.5 occ:0.80 O A:HOH600 2.3 44.8 1.0 O A:MET26 2.3 33.0 1.0 O A:GLY28 2.4 40.1 1.0 OE2 A:GLU70 2.5 37.8 1.0 O A:GLY30 2.5 37.5 1.0 OE1 A:GLU70 2.5 35.2 1.0 O A:HOH594 2.7 57.7 1.0 CD A:GLU70 2.9 35.5 1.0 C A:MET26 3.4 33.3 1.0 C A:GLY28 3.6 40.2 1.0 C A:GLY30 3.6 37.9 1.0 N A:GLY30 4.0 40.1 1.0 CA A:MET26 4.1 31.8 1.0 C A:LEU29 4.2 40.5 1.0 N A:GLY28 4.2 38.7 1.0 OG1 A:THR31 4.3 37.0 1.0 C A:LYS27 4.3 37.0 1.0 CG A:GLU70 4.4 34.7 1.0 CA A:GLY30 4.4 39.0 1.0 O A:LYS27 4.5 37.7 1.0 N A:LYS27 4.5 34.2 1.0 O A:HOH568 4.5 37.7 1.0 CA A:GLY28 4.5 39.4 1.0 N A:LEU29 4.5 40.1 1.0 CA A:LEU29 4.5 40.7 1.0 CB A:MET26 4.6 31.4 1.0 N A:THR31 4.6 37.1 1.0 O A:LEU29 4.8 41.6 1.0 CA A:LYS27 4.8 36.4 1.0 CA A:THR31 4.8 35.7 1.0

Calcium binding site 3 out of 7 in the Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca403 b:42.1 occ:0.60 O A:THR227 2.7 24.5 1.0 O A:ASP224 2.7 24.9 1.0 OE1 A:GLU232 3.0 32.8 1.0 O A:HOH640 3.1 47.6 1.0 OD1 A:ASP224 3.1 33.1 1.0 CD A:GLU232 3.6 33.0 1.0 C A:ASP224 3.7 23.9 1.0 OE2 A:GLU232 3.7 35.3 1.0 C A:THR227 3.8 24.6 1.0 CG A:ASP224 3.9 28.9 1.0 CA A:SER228 4.0 27.2 1.0 CA A:ASP224 4.1 22.9 1.0 CB A:SER228 4.2 28.3 1.0 N A:SER228 4.4 25.8 1.0 OD2 A:ASP224 4.6 33.6 1.0 CB A:ASP224 4.6 23.5 1.0 CG A:GLU232 4.7 25.0 1.0 O A:ILE223 4.9 20.0 1.0 N A:ARG225 4.9 23.1 1.0 N A:THR227 5.0 23.4 1.0

Calcium binding site 4 out of 7 in the Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca404 b:43.6 occ:0.50 O A:GLY261 2.0 48.2 1.0 O A:MET257 2.4 47.4 1.0 OD2 A:ASP301 2.5 45.4 1.0 O A:GLY259 2.5 55.6 1.0 OD1 A:ASP301 2.9 48.0 1.0 CG A:ASP301 3.0 45.1 1.0 C A:GLY261 3.1 48.2 1.0 N A:GLY261 3.6 51.8 1.0 C A:MET257 3.6 47.2 1.0 C A:GLY259 3.7 55.0 1.0 CA A:GLY261 3.8 49.9 1.0 OG1 A:THR262 4.1 45.9 1.0 N A:THR262 4.2 46.3 1.0 N A:GLY259 4.2 54.0 1.0 CA A:MET257 4.3 44.6 1.0 C A:ALA260 4.3 53.1 1.0 CB A:ASP301 4.5 44.1 1.0 CA A:THR262 4.6 44.0 1.0 CA A:GLY259 4.6 54.6 1.0 C A:LYS258 4.6 52.9 1.0 N A:LYS258 4.7 49.4 1.0 N A:ALA260 4.7 54.6 1.0 CA A:ALA260 4.8 53.7 1.0 CB A:MET257 4.8 44.5 1.0 CA A:LYS258 4.9 52.1 1.0 CB A:THR262 4.9 44.7 1.0 O A:ALA260 5.0 53.4 1.0

Calcium binding site 5 out of 7 in the Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca405 b:41.1 occ:0.40 O A:LYS68 2.3 33.8 1.0 O A:LEU71 2.4 32.2 1.0 C A:LYS68 3.5 32.9 1.0 C A:LEU71 3.6 31.7 1.0 OE1 A:GLU76 3.6 43.2 1.0 CG A:GLU76 4.2 33.6 1.0 CD A:GLU76 4.2 39.9 1.0 CA A:THR72 4.3 34.4 1.0 CA A:LYS68 4.3 31.9 1.0 N A:THR72 4.4 33.2 1.0 N A:SER69 4.5 33.5 1.0 N A:LEU71 4.6 31.8 1.0 CA A:SER69 4.7 34.5 1.0 CA A:LEU71 4.7 32.0 1.0 C A:SER69 4.7 34.4 1.0 O A:SER69 4.8 35.4 1.0 CB A:GLU76 4.9 30.5 1.0 CB A:THR72 4.9 34.7 1.0 CB A:LYS68 4.9 31.8 1.0

Calcium binding site 6 out of 7 in the Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca407 b:49.0 occ:0.40 O A:THR31 2.4 35.1 1.0 OE1 A:GLU33 2.7 37.4 1.0 OE2 A:GLU33 3.2 38.1 1.0 CD A:GLU33 3.3 36.5 1.0 C A:THR31 3.6 35.2 1.0 N A:THR31 4.1 37.1 1.0 CA A:THR31 4.4 35.7 1.0 CB A:THR31 4.6 36.3 1.0 O A:HOH635 4.6 47.5 1.0 N A:ASP32 4.7 33.2 1.0 N A:GLU33 4.7 32.8 1.0 CG A:GLU33 4.8 35.3 1.0 CA A:ASP32 4.8 33.0 1.0

Calcium binding site 7 out of 7 in the Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 7 of Annexin V Under 2.0 Mpa Pressure of Nitrous Oxide within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca408 b:50.2 occ:0.50 N A:THR187 2.9 18.2 1.0 O A:THR187 3.2 19.9 1.0 CA A:GLY186 3.4 18.0 1.0 C A:GLY186 3.6 18.7 1.0 C A:THR187 4.0 19.7 1.0 CA A:THR187 4.0 18.6 1.0 CB A:THR187 4.5 19.3 1.0 OE2 A:GLU189 4.6 35.7 1.0 OE1 A:GLU189 4.7 28.4 1.0 O A:TRP185 4.7 19.3 1.0 N A:GLY186 4.8 16.9 1.0 CE A:LYS184 4.8 28.9 1.0 O A:GLY186 4.8 19.0 1.0 OG1 A:THR187 5.0 20.0 1.0

N.Colloc'h, J.Sopkova-De Oliveira Santos, P.Retailleau, B.Langlois D'estainto, B.Gallois, A.Brisson, J.J.Risso, M.Lemaire, J.H.Abraini. Protein Crystallography Under Xenon and Nitrous Oxide Pressure: Comparison with in Vivo Pharmacology Studies and Implications For the Mechanism of Inhaled Anesthetic Action Biophys.J. V. 92 217 2007.
ISSN: ISSN 0006-3495
PubMed: 17028130
DOI: 10.1529/BIOPHYSJ.106.093807