Calcium in PDB 2j1e: High Resolution Crystal Structure of CBM32 From A N-Acetyl- Beta-Hexosaminidase in Complex with Lacnac

The structure of High Resolution Crystal Structure of CBM32 From A N-Acetyl- Beta-Hexosaminidase in Complex with Lacnac, PDB code: 2j1e was solved by E.Ficko-Blean, A.B.Boraston, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.4
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	77.377, 77.377, 73.421, 90.00, 90.00, 90.00
R / Rfree (%)	17.5 / 25.2

The binding sites of Calcium atom in the High Resolution Crystal Structure of CBM32 From A N-Acetyl- Beta-Hexosaminidase in Complex with Lacnac (pdb code 2j1e). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the High Resolution Crystal Structure of CBM32 From A N-Acetyl- Beta-Hexosaminidase in Complex with Lacnac, PDB code: 2j1e:

Calcium binding site 1 out of 1 in the High Resolution Crystal Structure of CBM32 From A N-Acetyl- Beta-Hexosaminidase in Complex with Lacnac


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of High Resolution Crystal Structure of CBM32 From A N-Acetyl- Beta-Hexosaminidase in Complex with Lacnac within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1770 b:23.6 occ:1.00 O A:ALA761 2.6 22.8 1.0 O A:PHE647 2.6 22.2 1.0 O A:ASP652 2.6 24.4 1.0 O A:THR655 2.8 22.8 1.0 OD2 A:ASP650 2.9 23.7 1.0 OG1 A:THR655 2.9 24.5 1.0 O A:ASP650 3.2 25.7 1.0 C A:THR655 3.6 23.5 1.0 C A:ASP652 3.7 25.3 1.0 C A:ALA761 3.7 23.1 1.0 C A:PHE647 3.8 20.0 1.0 N A:ASP652 3.9 24.0 1.0 CG A:ASP650 4.0 24.9 1.0 CB A:THR655 4.1 24.6 1.0 C A:ASP650 4.1 25.2 1.0 CA A:THR655 4.1 24.6 1.0 CA A:ASP652 4.2 25.5 1.0 N A:THR655 4.2 25.4 1.0 CB A:ASP652 4.2 24.8 1.0 CA A:ALA761 4.3 22.7 1.0 N A:PHE656 4.4 20.9 1.0 OD1 A:ASP650 4.5 27.2 1.0 O A:HOH2065 4.6 28.7 1.0 CA A:ALA648 4.6 19.5 1.0 N A:ALA648 4.6 19.6 1.0 C A:GLY651 4.7 25.2 1.0 CA A:PHE647 4.7 20.0 1.0 N A:ASP650 4.7 24.0 1.0 CB A:ALA761 4.7 21.6 1.0 CA A:PHE656 4.8 20.5 1.0 N A:MET653 4.8 26.2 1.0 N A:GLY651 4.8 25.0 1.0 N A:GLU762 4.8 23.0 1.0 CA A:ASP650 4.9 24.8 1.0 CB A:PHE647 4.9 18.8 1.0 CA A:GLY651 5.0 24.7 1.0

E.Ficko-Blean, A.B.Boraston. The Interaction of A Carbohydrate-Binding Module From A Clostridium Perfringens N-Acetyl-Beta- Hexosaminidase with Its Carbohydrate Receptor J.Biol.Chem. V. 281 37748 2006.
ISSN: ISSN 0021-9258
PubMed: 16990278
DOI: 10.1074/JBC.M606126200