Calcium in PDB 2j5z: H-Ficolin Complexed to Galactose

The structure of H-Ficolin Complexed to Galactose, PDB code: 2j5z was solved by V.Garlatti, C.Gaboriaud, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.00 / 1.73
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	62.200, 62.200, 345.490, 90.00, 90.00, 90.00
R / Rfree (%)	20.2 / 23.3

The binding sites of Calcium atom in the H-Ficolin Complexed to Galactose (pdb code 2j5z). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the H-Ficolin Complexed to Galactose, PDB code: 2j5z:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the H-Ficolin Complexed to Galactose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of H-Ficolin Complexed to Galactose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1277 b:26.6 occ:1.00 O A:SER220 2.3 30.1 1.0 OD1 A:ASP216 2.3 26.2 1.0 O A:HOH2127 2.4 29.7 1.0 O A:HOH2123 2.4 32.2 1.0 O A:SER218 2.5 28.5 1.0 OD2 A:ASP214 2.5 24.4 1.0 OD1 A:ASP214 2.5 24.4 1.0 CG A:ASP214 2.8 25.9 1.0 CG A:ASP216 3.3 25.0 1.0 C A:SER220 3.4 30.4 1.0 C A:SER218 3.6 29.2 1.0 OD2 A:ASP216 3.7 24.3 1.0 N A:SER218 4.1 27.7 1.0 N A:SER220 4.1 31.0 1.0 O A:ASP216 4.2 23.5 1.0 N A:ASN221 4.2 29.3 1.0 CA A:SER218 4.2 28.8 1.0 CA A:SER220 4.3 31.2 1.0 CB A:ASP214 4.3 24.3 1.0 C A:ASN219 4.4 31.4 1.0 C A:ASP216 4.4 23.1 1.0 CA A:ASN221 4.4 28.4 1.0 N A:ASP216 4.4 22.2 1.0 CB A:SER218 4.4 29.1 1.0 O A:HOH2124 4.5 34.9 1.0 CB A:ASP216 4.6 23.6 1.0 N A:ASN219 4.6 29.6 1.0 CA A:ASP216 4.6 22.6 1.0 O A:ASN219 4.8 32.0 1.0 CA A:ASN219 4.8 30.8 1.0 N A:SER217 5.0 24.2 1.0

Calcium binding site 2 out of 3 in the H-Ficolin Complexed to Galactose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of H-Ficolin Complexed to Galactose within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1277 b:14.4 occ:1.00 OD1 B:ASP216 2.3 14.5 1.0 O B:SER218 2.4 14.1 1.0 O B:HOH2135 2.4 18.4 1.0 O B:SER220 2.4 14.4 1.0 O B:HOH2127 2.4 17.1 1.0 OD2 B:ASP214 2.4 15.5 1.0 OD1 B:ASP214 2.4 13.8 1.0 CG B:ASP214 2.8 15.1 1.0 CG B:ASP216 3.3 15.9 1.0 C B:SER220 3.4 13.1 1.0 C B:SER218 3.5 14.7 1.0 OD2 B:ASP216 3.7 14.2 1.0 N B:SER218 4.1 14.9 1.0 C B:ASN219 4.1 14.1 1.0 CA B:SER218 4.2 14.5 1.0 N B:SER220 4.2 13.5 1.0 O B:HOH2128 4.2 23.6 1.0 O B:ASP216 4.2 16.3 1.0 N B:ASN221 4.2 12.8 1.0 CA B:ASN221 4.2 13.2 1.0 O B:ASN219 4.3 16.3 1.0 CB B:ASP214 4.3 15.0 1.0 N B:ASP216 4.3 13.8 1.0 CB B:SER218 4.3 13.9 1.0 O B:HOH2140 4.3 31.5 1.0 CA B:SER220 4.4 14.1 1.0 C B:ASP216 4.4 14.3 1.0 N B:ASN219 4.4 12.7 1.0 CB B:ASP216 4.5 13.7 1.0 CA B:ASN219 4.6 13.6 1.0 CA B:ASP216 4.6 14.0 1.0 OG B:SER218 4.8 14.3 1.0 N B:SER217 5.0 13.8 1.0

Calcium binding site 3 out of 3 in the H-Ficolin Complexed to Galactose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of H-Ficolin Complexed to Galactose within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca1277 b:42.0 occ:1.00 OD1 C:ASP216 2.2 39.4 1.0 O C:SER220 2.3 42.0 1.0 OD2 C:ASP214 2.3 38.6 1.0 O C:SER218 2.4 45.0 1.0 O C:HOH2084 2.4 41.8 1.0 O C:HOH2087 2.4 39.6 1.0 OD1 C:ASP214 2.6 36.3 1.0 CG C:ASP214 2.8 37.4 1.0 CG C:ASP216 3.2 39.7 1.0 C C:SER218 3.4 44.6 1.0 C C:SER220 3.5 43.0 1.0 OD2 C:ASP216 3.6 38.8 1.0 O C:ASP216 3.9 41.1 1.0 N C:SER220 4.0 44.3 1.0 N C:SER218 4.1 43.5 1.0 CA C:SER218 4.1 44.2 1.0 C C:ASN219 4.2 44.5 0.5 O C:HOH2085 4.3 41.1 1.0 CB C:ASP214 4.3 38.1 1.0 C C:ASP216 4.3 41.1 1.0 CA C:SER220 4.3 44.0 1.0 N C:ASP216 4.3 39.1 1.0 CB C:SER218 4.4 43.9 1.0 N C:ASN219 4.4 44.6 0.5 N C:ASN219 4.4 44.7 0.5 C C:ASN219 4.4 44.6 0.5 N C:ASN221 4.4 43.0 1.0 CB C:ASP216 4.5 39.5 1.0 CA C:ASN221 4.5 42.9 1.0 CA C:ASN219 4.6 44.5 0.5 CA C:ASP216 4.6 40.0 1.0 CA C:ASN219 4.6 44.7 0.5 O C:ASN219 4.7 44.6 0.5 OG C:SER218 4.8 43.3 1.0

V.Garlatti, N.Belloy, L.Martin, M.Lacroix, M.Matsushita, Y.Endo, T.Fujita, J.C.Fontecilla-Camps, G.J.Arlaud, N.M.Thielens, C.Gaboriaud. Structural Insights Into the Innate Immune Recognition Specificities of L- and H-Ficolins. Embo J. V. 26 623 2007.
ISSN: ISSN 0261-4189
PubMed: 17215869
DOI: 10.1038/SJ.EMBOJ.7601500