Calcium in PDB 2jep: Native Family 5 Xyloglucanase From Paenibacillus Pabuli

All present enzymatic activity of Native Family 5 Xyloglucanase From Paenibacillus Pabuli:
3.2.1.151;

The structure of Native Family 5 Xyloglucanase From Paenibacillus Pabuli, PDB code: 2jep was solved by T.M.Gloster, F.M.Ibatullin, K.Macauley, J.M.Eklof, S.Roberts, J.P.Turkenburg, M.E.Bjornvad, P.L.Jorgensen, S.Danielsen, K.Johansen, T.V.Borchert, K.S.Wilson, H.Brumer, G.J.Davies, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	63.25 / 1.4
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	84.416, 89.574, 90.397, 90.00, 90.00, 90.00
R / Rfree (%)	11.5 / 14.4

The binding sites of Calcium atom in the Native Family 5 Xyloglucanase From Paenibacillus Pabuli (pdb code 2jep). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Native Family 5 Xyloglucanase From Paenibacillus Pabuli, PDB code: 2jep:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Native Family 5 Xyloglucanase From Paenibacillus Pabuli


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Native Family 5 Xyloglucanase From Paenibacillus Pabuli within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1398 b:8.4 occ:0.75 O A:HOH2630 2.4 15.4 1.0 O A:HOH2245 2.4 12.7 1.0 O A:HOH2541 2.4 9.3 1.0 O A:HOH2345 2.4 12.9 1.0 O A:HOH2631 2.5 13.9 1.0 O2 A:EDO1396 2.5 12.0 1.0 O1 A:EDO1396 2.5 11.9 1.0 O A:HOH2628 2.5 15.2 1.0 C1 A:EDO1396 3.4 11.7 1.0 C2 A:EDO1396 3.5 11.8 1.0 O A:HOH2540 4.0 10.1 1.0 OE1 A:GLU182 4.5 8.2 1.0 O A:HOH2262 4.5 35.2 1.0 O A:HOH2261 4.6 26.3 1.0 OH A:TYR135 4.6 8.1 1.0 OE1 A:GLU323 4.6 7.2 1.0 O A:HOH2629 4.6 17.1 1.0 O A:HOH2138 4.7 44.0 1.0 O A:HOH2232 4.7 18.6 1.0 O A:HOH2025 4.9 13.7 1.0 OE2 A:GLU182 4.9 7.5 1.0 O A:HOH2137 4.9 23.2 0.5 O A:HOH2263 5.0 22.0 0.5 OH A:TYR266 5.0 8.0 1.0

Calcium binding site 2 out of 2 in the Native Family 5 Xyloglucanase From Paenibacillus Pabuli


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Native Family 5 Xyloglucanase From Paenibacillus Pabuli within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1397 b:8.9 occ:0.75 O B:HOH2235 2.4 13.7 1.0 O B:HOH2642 2.4 14.9 1.0 O B:HOH2541 2.4 9.7 1.0 O2 B:EDO1396 2.5 12.5 1.0 O B:HOH2641 2.5 15.0 1.0 O B:HOH2332 2.5 13.7 1.0 O1 B:EDO1396 2.5 11.7 1.0 O B:HOH2640 2.6 14.2 1.0 C1 B:EDO1396 3.4 12.0 1.0 C2 B:EDO1396 3.4 11.5 1.0 O B:HOH2542 4.0 10.1 1.0 O B:HOH2250 4.4 34.6 1.0 OE1 B:GLU182 4.5 9.4 1.0 OH B:TYR135 4.5 8.5 1.0 O B:HOH2643 4.6 17.2 1.0 O B:HOH2207 4.6 22.5 1.0 OE1 B:GLU323 4.6 7.7 1.0 O B:HOH2216 4.7 20.1 1.0 O B:HOH2018 4.9 13.8 1.0 OE2 B:GLU182 4.9 8.1 1.0 O B:HOH2128 5.0 25.2 0.5 OH B:TYR266 5.0 8.4 1.0 O B:HOH2028 5.0 14.9 1.0

T.M.Gloster, F.M.Ibatullin, K.Macauley, J.M.Eklof, S.Roberts, J.P.Turkenburg, M.E.Bjornvad, P.L.Jorgensen, S.Danielsen, K.Johansen, T.V.Borchert, K.S.Wilson, H.Brumer, G.J.Davies. Characterization and Three-Dimensional Structures of Two Distinct Bacterial Xyloglucanases From Families GH5 and GH12. J.Biol.Chem. V. 282 19177 2007.
ISSN: ISSN 0021-9258
PubMed: 17376777
DOI: 10.1074/JBC.M700224200